cDNA Clone NLEL (E.coli)

• Synonyms: secreted effector protein
• Plasmid: pGEX TEV NLEL 59-end (E.coli)
• Parent Plasmid: pGEX TEV
• Expressed: GST TEV NLEL 59-end (E.coli)
• Genbank: NP_309587.1
• Tag: GST
• Species: Escherichia coli

Sequence of Insert

GGATCCAACGGCGAAACCCTGAGCATTAGCGAACCGATTACCACCCTGCCGGATCTGCTGCCGGGCAGCCTGAAAGAACTGGTGCTGAACGGCTGCACCGAACTGAAAAGCATTAACTGCCTGCCGCCGAACCTGAGCAGCCTGAGCATGGTGGGCTGCAGCAGCCTGGAAGTGATTAACTGCAGCATTCCGGAAAACGTGATTAACCTGAGCCTGTGCCATTGCAGCAGCCTGAAACATATTGAAGGCAGCTTTCCGGAAGCGCTGCGCAACAGCGTGTATCTGAACGGCTGCAACAGCCTGAACGAAAGCCAGTGCCAGTTTCTGGCGTATGATGTGAGCCAGGGCCGCGCGTGCCTGAGCAAAGCGGAACTGACCGCGGATCTGATTTGGCTGAGCGCGAACCGCACCGGCGAAGAAAGCGCGGAAGAACTGAACTATAGCGGCTGCGATCTGAGCGGCCTGAGCCTGGTGGGCCTGAACCTGAGCAGCGTGAACTTTAGCGGCGCGGTGCTGGATGATACCGATCTGCGCATGAGCGATCTGAGCCAGGCGGTGCTGGAAAACTGCAGCTTTAAAAACAGCATTCTGAACGAATGCAACTTTTGCTATGCGAACCTGAGCAACTGCATTATTCGCGCGCTGTTTGAAAACAGCAACTTTAGCAACAGCAACCTGAAAAACGCGAGCTTTAAAGGCAGCAGCTATATTCAGTATCCGCCGATTCTGAACGAAGCGGATCTGACCGGCGCGATTATTATTCCGGGCATGGTGCTGAGCGGCGCGATTCTGGGCGATGTGAAAGAACTGTTTAGCGAAAAAAGCAACACCATTAACCTGGGCGGCTGCTATATTGATCTGAGCGATATTCAGGAAAACATTCTGAGCGTGCTGGATAACTATACCAAAAGCAACAAAAGCATTCTGCTGACCATGAACACCAGCGATGATAAATATAACCATGATAAAGTGCGCGCGGCGGAAGAACTGATTAAAAAAATTAGCCTGGATGAACTGGCGGCGTTTCGCCCGTATGTGAAAATGAGCCTGGCGGATAGCTTTAGCATTCATCCGTATCTGAACAACGCGAACATTCAGCAGTGGCTGGAACCGATTTGCGATGATTTTTTTGATACCATTATGAGCTGGTTTAACAACAGCATTATGATGTATATGGAAAACGGCAGCCTGCTGCAGGCGGGCATGTATTTTGAACGCCATCCGGGCGCGATGGTGAGCTATAACAGCAGCTTTATTCAGATTGTGATGAACGGCAGCCGCCGCGATGGCATGCAGGAACGCTTTCGCGAACTGTATGAAGTGTATCTGAAAAACGAAAAAGTGTATCCGGTGACCCAGCAGAGCGATTTTGGCCTGTGCGATGGCAGCGGCAAACCGGATTGGGATGATGATAGCGATCTGGCGTATAACTGGGTGCTGCTGAGCAGCCAGGATGATGGCATGGCGATGATGTGCAGCCTGAGCCATATGGTGGATATGCTGAGCCCGAACACCAGCACCAACTGGATGAGCTTTTTTCTGTATAAAGATGGCGAAGTGCAGAACACCTTTGGCTATAGCCTGAGCAACCTGTTTAGCGAAAGCTTTCCGATTTTTAGCATTCCGTATCATAAAGCGTTTAGCCAGAACTTTGTGAGCGGCATTCTGGATATTCTGATTAGCGATAACGAACTGAAAGAACGCTTTATTGAAGCGCTGAACAGCAACAAAAGCGATTATAAAATGATTGCGGATGATCAGCAGCGCAAACTGGCGTGCGTGTGGAACCCGTTTCTGGATGGCTGGGAACTGAACGCGCAGCATGTGGATATGATTATGGGCAGCCATGTGCTGAAAGATATGCCGCTGCGCAAACAGGCGGAAATTCTGTTTTGCCTGGGCGGCGTGTTTTGCAAATATAGCAGCAGCGATATGTTTGGCACCGAATATGATAGCCCGGAAATTCTGCGCCGCTATGCGAACGGCCTGATTGAACAGGCGTATAAAACCGATCCGCAGGTGTTTGGCAGCGTGTATTATTATAACGATATTCTGGATCGCCTGCAGGGCCGCAACAACGTGTTTACCTGCACCGCGGTGCTGACCGATATGCTGACCGAACATGCGAAAGAAAGCTTTCCGGAAATTTTTAGCCTGTATTATCCGGTGGCGTGGCGCTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDENLYFQGGSNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLSSLSMVGCSSLEVINCSIPENVINLSLCHCSSLKHIEGSFPEALRNSVYLNGCNSLNESQCQFLAYDVSQGRACLSKAELTADLIWLSANRTGEESAEELNYSGCDLSGLSLVGLNLSSVNFSGAVLDDTDLRMSDLSQAVLENCSFKNSILNECNFCYANLSNCIIRALFENSNFSNSNLKNASFKGSSYIQYPPILNEADLTGAIIIPGMVLSGAILGDVKELFSEKSNTINLGGCYIDLSDIQENILSVLDNYTKSNKSILLTMNTSDDKYNHDKVRAAEELIKKISLDELAAFRPYVKMSLADSFSIHPYLNNANIQQWLEPICDDFFDTIMSWFNNSIMMYMENGSLLQAGMYFERHPGAMVSYNSSFIQIVMNGSRRDGMQERFRELYEVYLKNEKVYPVTQQSDFGLCDGSGKPDWDDDSDLAYNWVLLSSQDDGMAMMCSLSHMVDMLSPNTSTNWMSFFLYKDGEVQNTFGYSLSNLFSESFPIFSIPYHKAFSQNFVSGILDILISDNELKERFIEALNSNKSDYKMIADDQQRKLACVWNPFLDGWELNAQHVDMIMGSHVLKDMPLRKQAEILFCLGGVFCKYSSSDMFGTEYDSPEILRRYANGLIEQAYKTDPQVFGSVYYYNDILDRLQGRNNVFTCTAVLTDMLTEHAKESFPEIFSLYYPVAWR*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX TEV NLEL 59-end (E.coli)65.4 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU39419
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: as described in http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0019331. Codon optimised for E. coli
• Low Temp: No