cDNA Clone Lag2

• Plasmid: pRS423-Lag2 promoter (1.5kb)-GFP-Lag2
• Parent Plasmid: pRS423
• Expressed: GFP-Lag2
• Tag: GFP
• Species: Saccharomyces cerevisiae

Sequence of Insert

GGGCCCGTGCTTCACTTTGACCATAAGCGTCTCCTTGGGCTTGGCAGGAGCATCGGTGGATGTTGGAGCTACGGGCTTCGTAACACTTTTCGACTTATCAGTAGAATAAAATCTCAAAGATAAAGGATAGTTGGGCTGTACTCCTTGCGCTCTCAAAGGCCAATGACACGAGGGAATAGTGGAAGCTATTAATCTAGGGCCTTGATTTTTAACAAAATACAAGCTTGCTTGACGTCTTGTCACACATGACGCTCTTGATGCGAAATTCAACATCTTAGTATATTATCTGTAGTGATAGAGATAGCGTATATTATGCGCTGCCTGAAATGCACTGCGCTGTTCATATTCATCACCGTGGCAATGACTGATCCATTTATTTGGAGATGATTCAACGAATAATTTTTCACCGCGGGATTATCGCCCAAGACGGGGTCGTTTGAGGGTAATACCGTGGCTAGCTTTATGGCTTGTGAAACTATTGCCAGTTATGTGGTTACGTATAATCTACCGTATGTGTGTGTGTATTTATTTATGTAGGTTGCTAATGCTTTGGTGATCGTTTAAAGGAACTTGGACGCAACCCTCCCACGGGTGCCAGGGCTTGAAATCTCTTTCAATGCTGCTATTTTGCGCGACTCTGGGTACACCTTATACCCCTTGTTTCTATGGTTAAACCAATTGAATCTCCAGCAGAGCTCGTGAGCAAATAGCTCGCCGAAGCCGAGCATCATGCCGTTGAGGAAGGGCAAAAGTAGGTTGATGGAGCACGAGCCTACAAAGGATACAAGGCTCTGCACAAGTGGGCTCTCGTCGGCACTCACTGTGTTCTGCACCTCCATACAGTCTTTGTCTTCTGATTCGTCATCTGACACGCTCTCCCAGAATCCCACAACCTCTGTCATATTTCTGTCTTGTGGCAGTTTCGTAAGAAGGGGGGTGATGTTTCTAATATTACAGTGATGTTCTATTGTTCAAGTAAAGTGTATGGCTGTTTCTTATGGCGCGGAATATACCACTTCGTTATAAGTGATTACATCTCGTAACGCATTCCTCTTGTTCAGTGGTAGGGGAGGCCGATGGTAGTGGAATAATACGCTTTTATGGGCCATTGGTATTACTATTTATCTTCGTTATAATAAATAGTACACGTTGCAGACATATTTGGAGGAGAGTTTTTAAAGTACTGTTGGGGTGCTCTTGCTGTGCATTGTAAGTAATCGTGTCCCAGTGGCCGAGTGGTTAAGGCGATGCCCTGCTATTTCCTCAGAAAAGCAATTAGGCATTGGGTTTTACCTGCGCAGGTTCGAATCCTGTCTGTGACGCATTTCTTTTTTGTGAATTCTTGCTTCTTATCCTTCTACAATCCTTTCGTGACATTTATTGAATCATCTCTACAAAATTTTTACACATAGCTAAGGTTAGCTTGTTCTACATTACACAAGTGGTAGCAAAATCGTTTACATTGCACTCAGCTATATCAACATCACGTTCTACCGCAACTAGTAGTCGAC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSGGGGGGGGTMSLHISKLIEQYRSTKDNDLKYMLLRQNFKINDIEDELAPLVNELLLPVLVEEQDMEILNLVSFQVLPDLVLSMISDPAAAQLGWVISLICDPLLNQSMIHANRSFVLIETLRNVLQKIENSPHLDYHQPVNSSLEFISKFIVEMKRHMCDVDAAQLSHSLSESNMLIYIESLNLLLKFSFFSDAASPSVMVTLPFDILNDVFTIAQDYSATNTNESIDRITEKLLLTSTQLTHPVDLENLCPKMKYNTLAAVSRIWYKFGPIVDKLFTNRLLPVLFPPQMGEECNVEDVLEIVHNFHPYFSIRRLKDNRPLLSDSTISQLREGLFGMLSILNDSLTRTQNENDHGSDNLIDSDDGFGSDNDPEQQAYLDELVSEGYDENMYDGDTDDEDADDINVEKNDEATKDITETNKILLIFSELHYPQEERFSELLVELQTKIAINTSLIDKILSKETTELPTHNGEIADLNEILNEVKGNKPIRKNVIFCTLAHTLSLQSGSELSVLQLSIEVIDHLLVKNHSNNITRGEQFQLIKLILPHLKTNKSFIDTLKAGNFTQKIDEGVTLRTMILSLLLQLFPLDYSMLGEILPTIARYSVRDKDLGVRDLSFQLLDQILRTYYNYLIGIDWEWYKDDFYQVLQETCIKKDINTNLLLQFPPYLPHD*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU23011
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: to enable direct comparison of 1.5kb Lag2 Pr with the 1kb upstream from ORF in SC23010