cDNA Clone Ddb1

• Plasmid: pFBDM-GST-Cullin4a; 6His-Ddb1
• Parent Plasmid: pFBDM
• Expressed: 6His-Ddb1
• Genbank: NM_001923
• Tag: 6His
• Species: Human

Sequence of Insert

GGTACCATGCATCATCACCATCACCATATGTCGTACAACTACGTGGTAACGGCCCAGAAGCCCACCGCCGTGAACGGCTGCGTGACCGGACACTTTACTTCGGCCGAAGACTTAAACCTGTTGATTGCCAAAAACACGAGATTAGAGATCTATGTGGTCACCGCCGAGGGGCTTCGGCCCGTCAAAGAGGTGGGCATGTATGGGAAGATTGCGGTCATGGAGCTTTTCAGGCCCAAGGGGGAGAGCAAGGACCTGCTGTTTATCTTGACAGCGAAGTACAATGCCTGCATCCTGGAGTATAAACAGAGTGGCGAGAGCATTGACATCATTACGCGAGCCCATGGCAATGTCCAGGACCGCATTGGCCGCCCCTCAGAGACCGGCATTATTGGCATCATTGACCCTGAGTGCCGGATGATTGGCCTGCGTCTCTATGATGGCCTTTTCAAGGTTATTCCACTAGATCGCGATAATAAAGAACTCAAGGCCTTCAACATCCGCCTGGAGGAGCTGCATGTCATTGATGTCAAGTTCCTATATGGTTGCCAAGCACCTACTATTTGCTTTGTCTACCAGGACCCTCAGGGGCGGCACGTAAAAACCTATGAGGTGTCTCTCCGAGAAAAGGAATTCAATAAGGGCCCTTGGAAACAGGAAAATGTCGAAGCTGAAGCTTCCATGGTGATCGCAGTCCCAGAGCCCTTTGGGGGGGCCATCATCATTGGACAGGAGTCAATCACCTATCACAATGGTGACAAATACCTGGCTATTGCCCCTCCTATCATCAAGCAAAGCACGATTGTGTGCCACAATCGAGTGGACCCTAATGGCTCAAGATACCTGCTGGGAGACATGGAAGGCCGGCTCTTCATGCTGCTTTTGGAGAAGGAGGAACAGATGGATGGCACCGTCACTCTCAAGGATCTCCGTGTAGAACTCCTTGGAGAGACCTCTATTGCTGAGTGCTTGACATACCTTGATAATGGTGTTGTGTTTGTCGGGTCTCGCCTGGGTGACTCCCAGCTTGTGAAGCTCAACGTTGACAGTAATGAACAAGGCTCCTATGTAGTGGCCATGGAAACCTTTACCAACTTAGGACCCATTGTCGATATGTGCGTGGTGGACCTGGAGAGGCAGGGGCAGGGGCAGCTGGTCACTTGCTCTGGGGCTTTCAAGGAAGGTTCTTTGCGGATCATCCGGAATGGAATTGGAATCCACGAGCATGCCAGCATTGACTTACCAGGCATCAAAGGATTATGGCCACTGCGGTCTGACCCTAATCGTGAGACTGATGACACTTTGGTGCTCTCTTTTGTGGGCCAGACAAGAGTTCTCATGTTAAATGGAGAGGAGGTAGAAGAAACCGAACTGATGGGTTTCGTGGATGATCAGCAGACTTTCTTCTGTGGCAACGTGGCTCATCAGCAGCTTATCCAGATCACTTCAGCATCGGTGAGGTTGGTCTCTCAAGAACCCAAAGCTCTGGTCAGTGAATGGAAGGAGCCTCAGGCCAAGAACATCAGTGTGGCCTCCTGCAATAGCAGCCAGGTGGTGGTGGCTGTAGGCAGGGCCCTCTACTATCTGCAGATCCATCCTCAGGAGCTCCGGCAGATCAGCCACACAGAGATGGAACATGAAGTGGCTTGCTTGGACATCACCCCATTAGGAGACAGCAATGGACTGTCCCCTCTTTGTGCCATTGGCCTCTGGACGGACATCTCGGCTCGTATCTTGAAGTTGCCCTCTTTTGAACTACTGCACAAGGAGATGCTGGGTGGAGAGATCATTCCTCGCTCCATCCTGATGACCACCTTTGAGAGTAGCCATTACCTCCTTTGTGCCTTGGGAGATGGAGCGCTTTTCTACTTTGGGCTCAACATTGAGACAGGTCTGTTGAGCGACCGTAAGAAGGTGACTTTGGGCACCCAGCCCACCGTATTGAGGACTTTTCGTTCTCTTTCTACCACCAACGTCTTTGCTTGTTCTGACCGCCCCACTGTCATCTATAGCAGCAACCACAAATTGGTCTTCTCAAATGTCAACCTCAAGGAAGTGAACTACATGTGTCCCCTCAATTCAGATGGCTATCCTGACAGCCTGGCGCTGGCCAACAATAGCACCCTCACCATTGGCACCATCGATGAGATCCAGAAGCTGCACATTCGCACAGTTCCCCTCTATGAGTCTCCAAGGAAGATCTGCTACCAGGAAGTGTCCCAGTGTTTCGGGGTCCTCTCCAGCCGCATTGAAGTCCAAGACACGAGTGGGGGCACGACAGCCTTGAGGCCCAGCGCTAGCACCCAGGCTCTGTCCAGCAGTGTAAGCTCCAGCAAGCTGTTCTCCAGCAGCACTGCTCCTCATGAGACCTCCTTTGGAGAAGAGGTGGAGGTGCACAACCTACTTATCATTGACCAACACACCTTTGAAGTGCTTCATGCCCACCAGTTTCTGCAGAATGAATATGCCCTCAGTCTGGTTTCCTGCAAGCTGGGCAAAGACCCCAACACTTACTTCATTGTGGGCACAGCAATGGTGTATCCTGAAGAGGCAGAGCCCAAGCAGGGTCGCATTGTGGTCTTTCAGTATTCGGATGGAAAACTACAGACTGTGGCTGAAAAGGAAGTGAAAGGGGCCGTGTACTCTATGGTGGAATTTAACGGGAAGCTGTTAGCCAGCATCAATAGCACGGTGCGGCTCTATGAGTGGACAACAGAGAAGGAGCTGCGCACTGAGTGCAACCACTACAACAACATCATGGCCCTCTACCTGAAGACCAAGGGCGACTTCATCCTGGTGGGCGACCTTATGCGCTCAGTGCTGCTGCTTGCCTACAAGCCCATGGAAGGAAACTTTGAAGAGATTGCTCGAGACTTTAATCCCAACTGGATGAGTGCTGTGGAAATCTTGGATGATGACAATTTTCTGGGGGCTGAAAATGCCTTTAACTTGTTTGTGTGTCAAAAGGATAGCGCTGCCACCACTGACGAGGAGCGGCAGCACCTCCAGGAGGTTGGTCTTTTCCACCTGGGCGAGTTTGTCAATGTCTTTTGCCACGGCTCTCTGGTAATGCAGAATCTGGGTGAGACTTCCACCCCCACACAAGGCTCGGTGCTCTTCGGCACGGTCAACGGCATGATAGGGCTGGTGACCTCACTGTCAGAGAGCTGGTACAACCTCCTGCTGGACATGCAGAATCGACTCAATAAAGTCATCAAAAGTGTGGGGAAGATCGAGCACTCCTTCTGGAGATCCTTTCACACCGAGCGGAAGACAGAACCAGCCACAGGTTTCATCGACGGTGACTTGATTGAGAGTTTCCTGGATATTAGCCGCCCCAAGATGCAGGAGGTGGTGGCAAACCTACAGTATGACGATGGCAGCGGTATGAAGCGAGAGGCCACTGCAGACGACCTCATCAAGGTTGTGGAGGAGCTAACTCGGATCCATTAGGGTACC

Amino Acid Sequence

MHHHHHHMSYNYVVTAQKPTAVNGCVTGHFTSAEDLNLLIAKNTRLEIYVVTAEGLRPVKEVGMYGKIAVMELFRPKGESKDLLFILTAKYNACILEYKQSGESIDIITRAHGNVQDRIGRPSETGIIGIIDPECRMIGLRLYDGLFKVIPLDRDNKELKAFNIRLEELHVIDVKFLYGCQAPTICFVYQDPQGRHVKTYEVSLREKEFNKGPWKQENVEAEASMVIAVPEPFGGAIIIGQESITYHNGDKYLAIAPPIIKQSTIVCHNRVDPNGSRYLLGDMEGRLFMLLLEKEEQMDGTVTLKDLRVELLGETSIAECLTYLDNGVVFVGSRLGDSQLVKLNVDSNEQGSYVVAMETFTNLGPIVDMCVVDLERQGQGQLVTCSGAFKEGSLRIIRNGIGIHEHASIDLPGIKGLWPLRSDPNRETDDTLVLSFVGQTRVLMLNGEEVEETELMGFVDDQQTFFCGNVAHQQLIQITSASVRLVSQEPKALVSEWKEPQAKNISVASCNSSQVVVAVGRALYYLQIHPQELRQISHTEMEHEVACLDITPLGDSNGLSPLCAIGLWTDISARILKLPSFELLHKEMLGGEIIPRSILMTTFESSHYLLCALGDGALFYFGLNIETGLLSDRKKVTLGTQPTVLRTFRSLSTTNVFACSDRPTVIYSSNHKLVFSNVNLKEVNYMCPLNSDGYPDSLALANNSTLTIGTIDEIQKLHIRTVPLYESPRKICYQEVSQCFGVLSSRIEVQDTSGGTTALRPSASTQALSSSVSSSKLFSSSTAPHETSFGEEVEVHNLLIIDQHTFEVLHAHQFLQNEYALSLVSCKLGKDPNTYFIVGTAMVYPEEAEPKQGRIVVFQYSDGKLQTVAEKEVKGAVYSMVEFNGKLLASINSTVRLYEWTTEKELRTECNHYNNIMALYLKTKGDFILVGDLMRSVLLLAYKPMEGNFEEIARDFNPNWMSAVEILDDDNFLGAENAFNLFVCQKDSAATTDEERQHLQEVGLFHLGEFVNVFCHGSLVMQNLGETSTPTQGSVLFGTVNGMIGLVTSLSESWYNLLLDMQNRLNKVIKSVGKIEHSFWRSFHTERKTEPATGFIDGDLIESFLDISRPKMQEVVANLQYDDGSGMKREATADDLIKVVEELTRIH*

• Vector Type: Baculovirus
• Antibiotic: Amp
• Full Snapgene Map: pFBDM-GST-Cullin4a; 6His-Ddb1100.85 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU21802
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No