cDNA Clone MAP4K5

• Synonyms: GCKR, KHS, KHS1, MAPKKKK5
• Plasmid: pFastbac-GST
• Parent Plasmid: pFastbacDual 6P1
• Expressed: GST-MAP4K5 (GCKR)
• Genbank: NP_006566.2
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGAGGCCCCGCTGCGGCCTGCCGCGGACATCCTGAGGCGGAACCCGCAGCAGGACTACGAACTCGTCCAGAGGGTCGGCAGCGGCACCTACGGGGACGTCTATAAGGCCAGAAATGTACACACAGGAGAGCTGGCTGCAGTAAAAATCATTAAATTGGAGCCTGGAGATGATTTTTCTTTGATTCAACAAGAAATATTTATGGTTAAAGAATGTAAACATTGTAACATCGTTGCCTACTTTGGGAGTTATCTTAGTCGGGAAAAACTATGGATTTGTATGGAATACTGTGGTGGCGGATCACTCCAAGATATTTACCATGTTACTGGACCATTATCAGAATTGCAAATAGCCTATGTATGCAGAGAAACCTTACAGGGTCTTGCCTATTTGCATACTAAAGGCAAAATGCATAGAGATATCAAAGGTGCTAATATTTTATTGACAGACCATGGCGATGTAAAATTAGCTGACTTTGGTGTGGCTGCAAAAATAACAGCTACCATTGCAAAACGAAAATCTTTCATTGGCACCCCTTACTGGATGGCCCCAGAAGTTGCAGCAGTAGAGAAGAATGGTGGCTACAACCAACTCTGTGATATCTGGGCAGTAGGAATAACAGCAATTGAACTTGGAGAACTTCAGCCACCTATGTTTGATCTCCACCCAATGAGGGCTCTCTTCTTAATGTCAAAAAGTAATTTTCAGCCTCCAAAACTAAAGGACAAAACAAAATGGTCATCAACATTCCATAATTTTGTCAAAATAGCACTAACCAAAAACCCAAAAAAAAGACCAACTGCTGAAAGACTTCTGACTCACACTTTTGTTGCACAGCCAGGTCTCTCTAGAGCCCTAGCAGTTGAACTGTTAGACAAAGTGAACAATCCAGATAACCACGCACATTACACTGAAGCAGATGACGATGACTTTGAGCCCCATGCAATCATTCGTCATACCATTAGATCTACAAACAGGAATGCCAGAGCTGAACGGACAGCTTCAGAAATAAATTTTGACAAATTACAATTTGAACCTCCTCTGAGAAAAGAAACAGAAGCACGAGATGAAATGGGATTGTCATCAGACCCAAATTTCATGTTACAGTGGAATCCTTTTGTTGATGGTGCAAATACTGGCAAATCAACCTCAAAACGTGCAATACCACCTCCCCTACCTCCTAAGCCAAGGATAAGCAGTTACCCTGAAGACAACTTTCCGGATGAAGAAAAAGCATCAACCATAAAACATTGTCCTGATTCAGAAAGCAGAGCTCCCCAAATTCTCAGAAGACAGAGTAGCCCAAGTTGTGGGCCTGTGGCAGAGACTTCTTCTATTGGAAATGGTGATGGTATTTCAAAACTGATGAGTGAAAATACAGAAGGATCAGCACAAGCACCACAGTTACCACGAAAAAAGGACAAACGAGACTTCCCTAAACCAGCCATCAATGGCCTTCCACCCACCCCAAAAGTTCTGATGGGAGCATGCTTTTCAAAAGTTTTTGATGGCTGTCCTTTGAAAATTAATTGTGCAACATCCTGGATACATCCTGATACAAAAGATCAGTACATTATTTTTGGAACTGAAGATGGTATTTACACACTGAATCTCAATGAGCTATATGAGGCAACGATGGAACAGTTATTTCCACGGAAGTGTACTTGGCTGTATGTTATCAATAATACTTTAATGTCATTATCAGAAGGAAAAACCTTTCAGCTCTACTCTCACAATCTTATAGCTTTGTTTGAACATGCCAAAAAACCAGGATTAGCTGCCCATATTCAAACTCACAGGTTTCCAGACCGAATACTACCAAGAAAATTCGCTTTAACAACAAAGATTCCTGATACAAAAGGCTGCCACAAATGTTGCATAGTCAGAAACCCTTACACGGGACATAAATACCTCTGTGGAGCTTTACAGTCTGGAATTGTTTTACTTCAGTGGTATGAGCCAATGCAGAAATTCATGTTGATAAAGCACTTTGATTTTCCTTTGCCAAGTCCTTTGAATGTTTTTGAAATGCTGGTGATACCTGAACAGGAATACCCTATGGTCTGTGTAGCTATTAGCAAAGGCACTGAATCGAATCAGGTAGTTCAGTTTGAGACAATCAATTTGAACTCTGCATCTTCATGGTTTACAGAAATTGGTGCAGGCAGTCAGCAGTTAGATTCCATTCATGTAACACAGTTGGAGAGAGATACCGTTTTAGTGTGTTTAGACAAATTTGTGAAAATTGTAAATCTACAAGGAAAATTAAAATCAAGTAAGAAACTGGCCTCTGAGTTAAGTTTTGATTTTCGCATTGAATCTGTAGTATGCCTTCAAGACAGTGTGTTGGCTTTCTGGAAACATGGGATGCAGGGTAAAAGCTTCAAGTCAGATGAGGTTACCCAGGAGATTTCAGATGAAACAAGAGTTTTCCGCTTATTAGGATCAGACAGGGTTGTCGTTTTGGAAAGTAGGCCAACAGAAAATCCTACTGCACACAGCAATCTCTACATCTTGGCTGGACATGAAAATAGTTACTAAGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSEAPLRPAADILRRNPQQDYELVQRVGSGTYGDVYKARNVHTGELAAVKIIKLEPGDDFSLIQQEIFMVKECKHCNIVAYFGSYLSREKLWICMEYCGGGSLQDIYHVTGPLSELQIAYVCRETLQGLAYLHTKGKMHRDIKGANILLTDHGDVKLADFGVAAKITATIAKRKSFIGTPYWMAPEVAAVEKNGGYNQLCDIWAVGITAIELGELQPPMFDLHPMRALFLMSKSNFQPPKLKDKTKWSSTFHNFVKIALTKNPKKRPTAERLLTHTFVAQPGLSRALAVELLDKVNNPDNHAHYTEADDDDFEPHAIIRHTIRSTNRNARAERTASEINFDKLQFEPPLRKETEARDEMGLSSDPNFMLQWNPFVDGANTGKSTSKRAIPPPLPPKPRISSYPEDNFPDEEKASTIKHCPDSESRAPQILRRQSSPSCGPVAETSSIGNGDGISKLMSENTEGSAQAPQLPRKKDKRDFPKPAINGLPPTPKVLMGACFSKVFDGCPLKINCATSWIHPDTKDQYIIFGTEDGIYTLNLNELYEATMEQLFPRKCTWLYVINNTLMSLSEGKTFQLYSHNLIALFEHAKKPGLAAHIQTHRFPDRILPRKFALTTKIPDTKGCHKCCIVRNPYTGHKYLCGALQSGIVLLQWYEPMQKFMLIKHFDFPLPSPLNVFEMLVIPEQEYPMVCVAISKGTESNQVVQFETINLNSASSWFTEIGAGSQQLDSIHVTQLERDTVLVCLDKFVKIVNLQGKLKSSKKLASELSFDFRIESVVCLQDSVLAFWKHGMQGKSFKSDEVTQEISDETRVFRLLGSDRVVVLESRPTENPTAHSNLYILAGHENSY

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU38642
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: H542Y compared to reference