cDNA Clone ACTN4

• Plasmid: pGEX6P-1-ACTN4 (actinin, alpha 4)
• Parent Plasmid: pGEX6P1
• Expressed: GST-ACTN4
• Genbank: NM_004924
• Tag: GST
• Species: Human

Sequence of Insert

gaattcatggtggactaccacgcggcgaaccagtcgtaccagtacggccccagcagcgcgggcaatggcgctggcggcgggggcagcatgggcgactacatggcccaggaggacgactgggaccgggacctgctgctggacccggcctgggagaagcagcagcgcaagaccttcacggcatggtgcaactcccacctgcggaaggcaggcacacagatcgagaacattgatgaggacttccgagacgggctcaagctcatgctgctcctggaggtcatatcaggggagcggttacctaagccggag
cgggggaagatgagagtgcacaaaatcaacaatgtgaacaaagcgctgga
ctttattgccagcaaaggcgtcaagctggtctccatcggggcagaagaga
ttgtggacggcaacgcaaagatgaccctgggaatgatctggaccatcatc
cttaggttcgccatccaggacatctccgtggaagagacctcggccaagga
agggctccttctctggtgccagagaaagacagccccgtataagaacgtca
atgtgcagaacttccacatcagctggaaggatggtcttgccttcaatgcc
ctgatccaccggcacagaccagagctgattgagtatgacaagctgaggaa
ggacgaccctgtcaccaacctgaacaatgccttcgaagtggctgagaaat
acctcgacatccccaagatgctggatgcagaggacatcgtgaacacggcc
cggcccgacgagaaggccataatgacctatgtgtccagcttctaccatgc
cttttcaggagcgcagaaggctgaaactgccgccaaccggatctgtaagg
tgctggctgtcaaccaagagaacgagcacctgatggaggactacgagaag
ctggccagcgacctcctggagtggatccggcgcaccatcccctggctgga
ggaccgtgtgccccaaaagactatccaggagatgcagcagaagctggagg
acttccgcgactaccggcgtgtgcacaagccgcccaaggtgcaggagaag
tgccagctggagatcaacttcaacacgctgcagaccaagctgcgcctcag
caaccggcccgccttcatgccctccgagggcaagatggtctcggacatca
acaatggctggcagcacttggagcaggctgagaagggctacgaggagtgg
ctgctgaatgagatccgcaggctggagcggctcgaccacctggcagagaa
gttccggcagaaggcctccatccacgaggcctggactgacgggaaggaag
ccatgctgaagcaccgggactacgagacggccacactatcggacatcaaa
gccctcattcgcaagcacgaggccttcgagagcgacctggctgcgcacca
ggaccgcgtggagcagatcgccgccattgcccaggagctcaacgagctgg
attactacgactcccacaatgtcaacacccggtgccagaagatctgtgac
cagtgggacgccctcggctctctgacacatagtcgcagggaagccctgga
gaaaacagagaagcagctggaggccatcgaccagctgcacctggaatacg
ccaagcgcgcggcccccttcaacaactggatggagagcgccatggaggac
ctccaggacatgttcatcgtccataccatcgaggagattgagggcctgat
ctcagcccatgaccagttcaagtccaccctgccggacgccgatagggagc
gcgaggccatcctggccatccacaaggaggcccagaggatcgctgagagc
aaccacatcaagctgtcgggcagcaacccctacaccaccgtcaccccgca
aatcatcaactccaagtgggagaaggtgcagcagctggtgccaaaacggg
accatgccctcctggaggagcagagcaagcagcagtccaacgagcacctg
cgccgccagttcgccagccaggccaatgttgtggggccctggatccagac
caagatggaggagatcgggcgcatctccattgagatgaacgggaccctgg
aggaccagctgagccacctgaagcagtatgaacgcagcatcgtggactac
aagcccaacctggacctgctggagcagcagcaccagctcatccaggaggc
cctcatcttcgacaacaagcacaccaactataccatggagcacatccgcg
tgggctgggagcagctgctcaccaccattgcccgcaccatcaacgaggtg
gagaaccagatcctcacccgcgacgccaagggcatcagccaggagcagat
gcaggagttccgggcgtccttcaaccacttcgacaaggatcatggcgggg
cgctggggcccgaggagttcaaggcctgcctcatcagcctgggctacgac
gtggagaacgaccggcagggtgaggccgagttcaaccgcatcatgagcct
ggtcgaccccaaccatagcggccttgtgaccttccaagccttcatcgact
tcatgtcgcgggagaccaccgacacggacacggctgaccaggtcatcgct
tccttcaaggtcttagcaggggacaagaacttcatcacagctgaggagct
gcggagagagctgccccccgaccaggccgagtactgcatcgcccgcatgg
cgccataccagggccctgacgccgtgcccggtgccctcgactacaagtcc
ttctccacggccttgtatggcgagagcgacctgtgagcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMVDYHAANQSYQYGPS
SAGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRK
AGTQIENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKAL
DFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAK
EGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLR
KDDPVTNLNNAFEVAEKYLDIPKMLDAEDIVNTARPDEKAIMTYVSSFYH
AFSGAQKAETAANRICKVLAVNQENEHLMEDYEKLASDLLEWIRRTIPWL
EDRVPQKTIQEMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRL
SNRPAFMPSEGKMVSDINNGWQHLEQAEKGYEEWLLNEIRRLERLDHLAE
KFRQKASIHEAWTDGKEAMLKHRDYETATLSDIKALIRKHEAFESDLAAH
QDRVEQIAAIAQELNELDYYDSHNVNTRCQKICDQWDALGSLTHSRREAL
EKTEKQLEAIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTIEEIEGL
ISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSNPYTTVTP
QIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQFASQANVVGPWIQ
TKMEEIGRISIEMNGTLEDQLSHLKQYERSIVDYKPNLDLLEQQHQLIQE
ALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQ
MQEFRASFNHFDKDHGGALGPEEFKACLISLGYDVENDRQGEAEFNRIMS
LVDPNHSGLVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNFITAEE
LRRELPPDQAEYCIARMAPYQGPDAVPGALDYKSFSTALYGESDL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5855
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 132046 Daltons