cDNA Clone TRAF3

• Plasmid: pEFIRES-P-eYFP C1-TRAF3 g279a, c285t, a291g
• Parent Plasmid: pEFIRES puro eYFPC1
• Expressed: eYFP-TRAF3 g279a, c285t, a291g
• Genbank: AAH75086.1
• Tag: YFP
• Species: Human

Sequence of Insert

GGATCCATGGAGTCGAGTAAAAAGATGGACTCTCCTGGCGCGCTGCAGAC
TAACCCGCCGCTAAAGCTGCACACTGACCGCAGTGCTGGGACGCCAGTTT
TTGTCCCTGAACAAGGAGGTTACAAGGAAAAGTTTGTGAAGACCGTGGAG
GACAAGTACAAGTGTGAGAAGTGCCACCTGGTGCTGTGCAGCCCGAAGCA
GACCGAGTGTGGGCACCGCTTCTGCGAGAGCTGCATGGCGGCCCTGCTGA
GCTCTTCAAGTCCAAAATGTACAGCGTGTCAAGAAAGCATTGTTAAGGAT
AAGGTGTTTAAGGATAATTGCTGCAAGAGAGAAATTCTGGCTCTTCAGAT
CTATTGTCGGAATGAAAGCAGAGGTTGTGCAGAGCAGTTAATGCTGGGAC
ATCTGCTGGTGCATTTAAAAAATGATTGCCATTTTGAAGAACTTCCATGT
GTGCGTCCTGACTGCAAAGAAAAGGTCTTGAGGAAAGACCTGCGAGACCA
CGTGGAGAAGGCGTGTAAATACCGGGAAGCCACATGCAGCCACTGCAAGA
GTCAGGTTCCGATGATCGCGCTGCAGAAACACGAAGACACCGACTGTCCC
TGCGTGGTGGTGTCCTGCCCTCACAAGTGCAGCGTCCAGACTCTCCTGAG
GAGCGAGTTGAGTGCACACTTGTCAGAGTGTGTCAATGCCCCCAGCACCT
GTAGTTTTAAGCGCTATGGCTGCGTTTTTCAGGGGACAAACCAGCAGATC
AAGGCCCACGAGGCCAGCTCCGCCGTGCAGCACGTCAACCTGCTGAAGGA
GTGGAGCAACTCGCTCGAAAAGAAGGTTTCCTTGTTGCAGAATGAAAGTG
TAGAAAAAAACAAGAGCATACAAAGTTTGCACAATCAGATATGTAGCTTT
GAAATTGAAATTGAGAGACAAAAGGAAATGCTTCGAAATAATGAATCCAA
AATCCTTCATTTACAGCGAGTGATAGACAGCCAAGCAGAGAAACTGAAGG
AGCTTGACAAGGAGATCCGGCCCTTCCGGCAGAACTGGGAGGAAGCAGAC
AGCATGAAGAGCAGCGTGGAGTCCCTCCAGAACCGCGTGACCGAGCTGGA
GAGCGTGGACAAGAGCGCGGGGCAAGTGGCTCGGAACACAGGCCTGCTGG
AGTCCCAGCTGAGCCGGCATGACCAGATGCTGAGTGTGCACGACATCCGC
CTAGCCGACATGGACCTGCGCTTCCAGGTCCTGGAGACCGCCAGCTACAA
TGGAGTGCTCATCTGGAAGATTCGCGACTACAAGCGGCGGAAGCAGGAGG
CCGTCATGGGGAAGACCCTGTCCCTTTACAGCCAGCCTTTCTACACTGGT
TACTTTGGCTATAAGATGTGTGCCAGGGTCTACCTGAACGGGGACGGGAT
GGGGAAGGGGACGCACTTGTCGCTGTTTTTTGTCATCATGCGTGGAGAAT
ATGATGCCCTGCTTCCTTGGCCGTTTAAGCAGAAAGTGACACTCATGCTG
ATGGATCAGGGGTCCTCTCGACGTCATTTGGGAGATGCATTCAAGCCCGA
CCCCAACAGCAGCAGCTTCAAGAAGCCCACTGGAGAGATGAATATCGCCT
CTGGCTGCCCAGTCTTTGTGGCCCAAACTGTTCTAGAAAATGGGACATAT
ATTAAAGATGATACAATTTTTATTAAAGTCATAGTGGATACTTCGGATCT
GCCCGATCCCTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSMESSKK
MDSPGALQTNPPLKLHTDRSAGTPVFVPEQGGYKEKFVKTVEDKYKCEKC
HLVLCSPKQTECGHRFCESCMAALLSSSSPKCTACQESIVKDKVFKDNCC
KREILALQIYCRNESRGCAEQLMLGHLLVHLKNDCHFEELPCVRPDCKEK
VLRKDLRDHVEKACKYREATCSHCKSQVPMIALQKHEDTDCPCVVVSCPH
KCSVQTLLRSELSAHLSECVNAPSTCSFKRYGCVFQGTNQQIKAHEASSA
VQHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQSLHNQICSFEIEIERQK
EMLRNNESKILHLQRVIDSQAEKLKELDKEIRPFRQNWEEADSMKSSVES
LQNRVTELESVDKSAGQVARNTGLLESQLSRHDQMLSVHDIRLADMDLRF
QVLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCA
RVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGSSRR
HLGDAFKPDPNSSSFKKPTGEMNIASGCPVFVAQTVLENGTYIKDDTIFI
KVIVDTSDLPDP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20612
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression vector for eYFP-TRAF3 g279a, c285t, a291g. EF1a promoter, IRES and puromycin selection cassette