cDNA Clone Cullin2

• Plasmid: pFBDual GST-Cullin2, 6His-Rbx1
• Parent Plasmid: pFastbacDual 6P1
• Expressed: GST-Cullin2
• Genbank: NM_003591
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCatgtctttgaaaccaagagtagtagattttgatgaaacatggaacaaacttttgacgacaataaaagccgtggtcatgttggaatacgtcgaaagagcaacatggaatgaccgtttctcagatatctatgctttatgtgtggcctatcctgaaccccttggagaaagactttatacagaaactaagatttttttggaaaatcatgttcggcatttgcataagagagttttggagtcagaagaacaagtacttgttatgtatcataggtactgggaagaatacagcaagggtgcagactat
atggactgcttatataggtatctcaacacccagtttattaaaaagaataa
attaacagaagcggaccttcagtatggctatggtggtgtagatatgaatg
aaccacttatggaaataggagagctagcattggatatgtggaggaaattg
atggttgaaccacttcaggccatccttatccgaatgctgctccgagaaat
caaaaatgatcgtggtggagaagacccaaaccagaaagtaatccatgggg
ttattaactcctttgttcatgttgaacagtataagaaaaaattcccctta
aagttttatcaggaaatttttgagtctccctttctgactgaaacaggaga
gtattacaaacaagaagcttcaaatttattacaagaatcaaactgctcac
agtatatggaaaaggttctaggtagattaaaagatgaagaaattcgatgt
cgaaaatacctacatccaagttcatatactaaggtgattcatgaatgtca
acaacgaatggtagcagaccacttacagtttttacatgcagaatgtcata
atataattcgacaagagaaaaaaaatgacatggcaaatatgtacgtctta
ctccgtgctgtgtccactggtttacctcatatgattcaggagctgcaaaa
ccacatccatgatgagggccttcgagcaaccagcaaccttactcaggaaa
acatgccaacactatttgtggagtcagttttggaagtgcatggtaaattt
gttcagcttatcaacactgttttgaatggtgatcagcattttatgagtgc
gttggataaggcccttacgtcagttgtaaattacagagaacctaagtctg
tttgcaaagcacctgaactgcttgctaagtactgtgacaacttactgaag
aagtcagcgaaagggatgacagagaatgaagtggaagacaggctcacgag
cttcatcacagtgttcaaatacattgatgacaaggacgtctttcaaaagt
tctacgcaagaatgctggcaaaacgtttaattcatgggttatccatgtct
atggactctgaagaagccatgatcaacaaattaaagcaagcctgtggtta
tgagtttaccagcaagctacatcggatgtatacagatatgagtgtcagcg
ctgatctcaacaataagttcaacaattttatcaaaaaccaagacacagta
atagatttgggaattagttttcaaatatatgttctacaggctggtgcgtg
gcctcttactcaggctccttcatctacgtttgcaattccccaggaattag
aaaaaagtgtacagatgtttgaattattttatagccaacatttcagtgga
aggaaacttacatggttacattatctgtgtacaggtgaagttaaaatgaa
ctatttgggcaaaccatatgtagccatggttacaacataccaaatggcag
ttcttcttgcctttaacaacagtgaaactgtcagttataaagagcttcag
gacagcactcagatgaatgaaaaggaactgacaaaaacaatcaaatcatt
acttgatgtgaaaatgattaaccatgattcagaaaaggaagatattgatg
cagaatcttcgttttcattaaatatgaactttagcagtaaaagaacaaaa
tttaaaattactacatcaatgcagaaagacacaccacaagaaatggagca
gactagaagtgcagttgatgaggaccggaaaatgtatctccaagctgcta
tagttcgtatcatgaaagcacgaaaagtgcttcggcacaatgcccttatt
caagaggtgattagccagtcaagagctaggtttaatcccagtatcagcat
gattaagaagtgtattgaagttctgatagacaaacaatacatagaacgca
gccaggcgtcggcagatgaatacagctacgtcgcgtgagcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSLKPRVVDFDETWNKLLT
TIKAVVMLEYVERATWNDRFSDIYALCVAYPEPLGERLYTETKIFLENHV
RHLHKRVLESEEQVLVMYHRYWEEYSKGADYMDCLYRYLNTQFIKKNKLT
EADLQYGYGGVDMNEPLMEIGELALDMWRKLMVEPLQAILIRMLLREIKN
DRGGEDPNQKVIHGVINSFVHVEQYKKKFPLKFYQEIFESPFLTETGEYY
KQEASNLLQESNCSQYMEKVLGRLKDEEIRCRKYLHPSSYTKVIHECQQR
MVADHLQFLHAECHNIIRQEKKNDMANMYVLLRAVSTGLPHMIQELQNHI
HDEGLRATSNLTQENMPTLFVESVLEVHGKFVQLINTVLNGDQHFMSALD
KALTSVVNYREPKSVCKAPELLAKYCDNLLKKSAKGMTENEVEDRLTSFI
TVFKYIDDKDVFQKFYARMLAKRLIHGLSMSMDSEEAMINKLKQACGYEF
TSKLHRMYTDMSVSADLNNKFNNFIKNQDTVIDLGISFQIYVLQAGAWPL
TQAPSSTFAIPQELEKSVQMFELFYSQHFSGRKLTWLHYLCTGEVKMNYL
GKPYVAMVTTYQMAVLLAFNNSETVSYKELQDSTQMNEKELTKTIKSLLD
VKMINHDSEKEDIDAESSFSLNMNFSSKRTKFKITTSMQKDTPQEMEQTR
SAVDEDRKMYLQAAIVRIMKARKVLRHNALIQEVISQSRARFNPSISMIK
KCIEVLIDKQYIERSQASADEYSYVA*

• Vector Type: Baculovirus
• Antibiotic: Amp
• Full Snapgene Map: pFBDual GST-Cullin2, 6His-Rbx1203.99 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU21546
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: SC20032 (Cullin2) SC20946 (6HIS-Rbx1); (Cullin2 was subcloned BamHI-Not1 from SC20032 into the PPH driven cassette of SC20978 (pFBDual-GST6P-1 derived) thereby replacing the Cullin3 coding region). 6HIS-Rbx1 was previously subcloned Nhe1-Kpn1into the PP10 driven cassette.
• Low Temp: No