cDNA Clone HOIP

• Plasmid: pGEX6P2-HOIP delta 564-615
• Parent Plasmid: pGEX6P2
• Expressed: GST-HOIP delta 564-615
• Genbank: NM_017999
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGCCGGGGGAGGAAGAGGAGCGGGCCTTCCTGGTGGCCCG
CGAGGAGCTGGCGAGCGCCCTGAGGAGGGATTCCGGGCAGGCGTTTTCCC
TGGAGCAGCTCCGGCCGCTACTAGCCAGCTCTCTGCCGCTAGCCGCCCGC
TACCTGCAGCTGGACGCCGCACGCCTTGTCCGCTGCAACGCTCATGGGGA
GCCCCGAAACTACCTCAACACCCTGTCCACGGCTCTGAACATCCTGGAGA
AATACGGCCGCAACCTTCTCAGCCCTCAGCGGCCTCGGTACTGGCGTGGT
GTCAAGTTTAATAACCCTGTCTTTCGCAGCACGGTGGATGCTGTGCAGGG
GGGCCGAGATGTGCTGCGATTATATGGCTACACAGAGGAGCAACCAGATG
GGTTGAGCTTCCCCGAAGGGCAGGAGGAGCCAGATGAGCACCAGGTTGCT
ACAGTCACACTGGAAGTACTGCTGCTTCGGACAGAGCTCAGCCTGCTATT
GCAGAATACTCATCCAAGACAGCAGGCACTGGAGCAGCTGTTGGAAGACA
AGGTTGAAGATGATATGCTGCAGCTTTCAGAATTTGACCCCCTATTGAGA
GAGATTGCTCCTGGCCCCCTCACCACACCCTCTGTCCCAGGCTCCACTCC
TGGTCCCTGCTTCCTCTGTGGTTCTGCCCCAGGCACACTGCACTGCCCAT
CCTGTAAACAGGCCCTGTGTCCAGCCTGTGACCACCTGTTCCATGGACAC
CCATCCCGTGCTCATCACCTCCGCCAGACCCTGCCTGGGGTCCTGCAGGG
TACCCACCTGAGCCCCAGTTTACCTGCCTCAGCCCAACCACGGCCCCAGT
CGACCTCCCTGCTGGCCCTGGGAGACAGCTCTCTTTCTTCCCCTAATCCT
GCAAGTGCTCATTTGCCCTGGCACTGTGCTGCCTGTGCCATGCTAAATGA
GCCTTGGGCAGTGCTCTGTGTGGCCTGTGATCGGCCCCGAGGCTGTAAGG
GGTTGGGGTTGGGAACTGAGGGTCCCCAAGGAACTGGAGGCCTAGAACCT
GATCTTGCACGGGGTCGGTGGGCCTGCCAGAGCTGTACCTTTGAGAATGA
GGCAGCTGCTGTGCTATGTTCCATATGTGAGCGACCTCGGCTGGCCCAGC
CTCCCAGCTTGGTGGTGGATTCCCGAGATGCTGGCATTTGCCTGCAACCC
CTTCAGCAGGGGGATGCTTTGCTGGCCTCTGCCCAGAGTCAAGTCTGGTA
CTGTATTCACTGTACCTTCTGCAACTCGAGCCCTGGCTGGGTGTGTGTTA
TGTGCAACCGGACTAGTAGCCCCATTCCAGCACAACATGCCCCCCGGCCC
TATGCCAGCTCTTTGGAAAAGGGACCCCCCAAGCCTGGGCCCCCACGACG
CCTTAGTGCCCCCCTGCCCAGTTCCTGTGGAGATCCTGAGAAGCAGCGCC
AAGACAAGATGCGGGAAGAAGGCCTCCAGCTAGTGAGCATGATCCGGGAA
GGGGAAGCCGCAGGTGCCTGTCCAGAGGAGATCTTCTCGGCTCTGCAGTA
CTCGGGCACTGAGGTGCCTCTGCAGTGGTTGCGCTCAGAACTGCCCTACG
TCCTGGAGATGGTGGCTGAGCTGGCTGGACAGCAGGACCCTGGGCTGGGT
GCCTTTTCCTGTCAGGAGGCCCGGAGAGCCTGGCTGGATCGTCATGGCCG
CCTAGAGCCCTTCCGCCAGCGCCTCTGGGACAGTGGCCCTGAGCCCACCC
CTTCCTGGGATGGGCCAGACAAGCAGAGCCTGGTCAGGCGGCTTTTGGCA
GTCTACGCACTCCCCAGCTGGGGCCGGGCAGAGCTGGCACTGTCACTGCT
GCAGGAGACACCCAGGAACTATGAGTTGGGGGATGTGGTAGAAGCTGTGA
GGCACAGCCAGGACCGGGCCTTCCTGCGCCGCTTGCTTGCCCAGGAGTGT
GCCGTGTGTGGCTGGGCCCTGCCCCACAACCGGATGCAGGCCCTGACTTC
CTGTGAGTGCACCATCTGTCCTGACTGCTTCCGCCAGCACTTCACCATCG
CCTTGAAGGAGAAGCACATCACAGACATGGTGTGCCCTGCCTGTGGCCGC
CCCGACCTCACCGATGACACACAGTTGCTCAGCTACTTCTCTACCCTTGA
CATCCAGCTTCGCGAGAGCCTAGAGCCAGATGCCTATGCGTTGTTCCATA
AGAAGCTGACCGAGGGTGTGCTGATGCGGGACCCCAAGTTCTTGTGGTGT
GCCCAGTGCTCCTTTGGCTTCATATATGAGCGTGAGCAGCTGGAGGCAAC
TTGTCCCCAGTGTCACCAGACCTTCTGTGTGCGCTGCAAGCGCCAGTGGG
AGGAGCAGCACCGAGGTCGGAGCTGTGAGGACTTCCAGAACTGGAAACGC
ATGAACGACCCAGAATACCAGGCCCAGGGCCTAGCAATGTATCTTCAGGA
AAACGGCATTGACTGCCCCAAATGCAAGTTCTCGTACGCCCTGGCCCGAG
GAGGCTGCATGCACTTTCACTGTACCCAGTGCCGCCACCAGTTCTGCAGC
GGCTGCTACAATGCCTTTTACGCCAAGAATAAATGTCCAGAGCCTAACTG
CAGGGTGAAAAAGTCCCTGCACGGCCACCACCCTCGAGACTGCCTCTTCT
ACCTGCGGGACTGGACTGCTCTCCGGCTTCAGAAGCTGCTACAGGACAAT
AACGTCATGTTTAATACAGAGCCTCCAGCTGGGGCCCGGGCAGTCCCTGG
AGGCGGCTGCCGAGTGATAGAGCAGAAGGAGGTTCCCAATGGGCTCAGGG
ACGAAGCTTGTGGCAAGGAAACTCCAGCTGGCTATGCCGGCCTGTGCCAG
GCACACTACAAAGAGTATCTTGTGAGCCTCATCAATGCCCACTCGCTGGA
CCCAGCCACCTTGTATGAGGTGGAAGAGCTGGAGACGGCCACTGAGCGCT
ACCTGCACGTACGCCCCCAGCCTTTGGCTGGAGAGGATCCCCCTGCTTAC
CAGGCCCGCTTGTTACAGAAGCTGACAGAAGAGGTACCCTTGGGACAGAG
TATCCCCCGCAGGCGGAAGTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMPGEEEER
AFLVAREELASALRRDSGQAFSLEQLRPLLASSLPLAARYLQLDAARLVR
CNAHGEPRNYLNTLSTALNILEKYGRNLLSPQRPRYWRGVKFNNPVFRST
VDAVQGGRDVLRLYGYTEEQPDGLSFPEGQEEPDEHQVATVTLEVLLLRT
ELSLLLQNTHPRQQALEQLLEDKVEDDMLQLSEFDPLLREIAPGPLTTPS
VPGSTPGPCFLCGSAPGTLHCPSCKQALCPACDHLFHGHPSRAHHLRQTL
PGVLQGTHLSPSLPASAQPRPQSTSLLALGDSSLSSPNPASAHLPWHCAA
CAMLNEPWAVLCVACDRPRGCKGLGLGTEGPQGTGGLEPDLARGRWACQS
CTFENEAAAVLCSICERPRLAQPPSLVVDSRDAGICLQPLQQGDALLASA
QSQVWYCIHCTFCNSSPGWVCVMCNRTSSPIPAQHAPRPYASSLEKGPPK
PGPPRRLSAPLPSSCGDPEKQRQDKMREEGLQLVSMIREGEAAGACPEEI
FSALQYSGTEVPLQWLRSELPYVLEMVAELAGQQDPGLGAFSCQEARRAW
LDRHGRLEPFRQRLWDSGPEPTPSWDGPDKQSLVRRLLAVYALPSWGRAE
LALSLLQETPRNYELGDVVEAVRHSQDRAFLRRLLAQECAVCGWALPHNR
MQALTSCECTICPDCFRQHFTIALKEKHITDMVCPACGRPDLTDDTQLLS
YFSTLDIQLRESLEPDAYALFHKKLTEGVLMRDPKFLWCAQCSFGFIYER
EQLEATCPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRMNDPEYQAQGL
AMYLQENGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNK
CPEPNCRVKKSLHGHHPRDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAG
ARAVPGGGCRVIEQKEVPNGLRDEACGKETPAGYAGLCQAHYKEYLVSLI
NAHSLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKLTEE
VPLGQSIPRRRK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20907
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid for GST-HOIP delta aa564-615; ; Estimated MW of expressed protein : 141592 Daltons