cDNA Clone Den1

• Plasmid: pGEX6P-3-Den1
• Parent Plasmid: pGEX6P3
• Expressed: GST-Den1
• Genbank: NM_145204.3
• Tag: GST
• Species: Human

Sequence of Insert

GTCGACATGGACCCCGTAGTCTTGAGTTACATGGACAGTCTACTGCGGCA
ATCAGATGTCTCACTATTGGATCCGCCAAGCTGGCTCAATGACCATATTA
TTGGGTTTGCGTTTGAGTACTTTGCCAACAGTCAGTTTCATGACTGCTCT
GATCACGTCAGTTTCATCAGCCCTGAAGTCACCCAGTTCATCAAGTGCAC
TAGCAACCCAGCAGAGATTGCCATGTTCCTTGAACCACTGGACCTCCCCA
ACAAGAGAGTTGTATTTTTAGCCATCAATGATAACTCCAACCAGGCAGCT
GGAGGAACCCACTGGAGTTTATTGGTCTACCTCCAAGATAAAAATAGCTT
TTTTCATTATGATTCCCATAGCAGGAGCAACTCAGTTCACGCAAAGCAGG
TAGCAGAGAAACTGGAGGCTTTCTTAGGCAGAAAAGGAGACAAACTGGCC
TTTGTGGAAGAGAAAGCCCCTGCCCAACAAAACAGCTATGACTGTGGGAT
GTACGTGATATGTAACACTGAGGCCTTGTGTCAGAACTTCTTTAGGCAAC
AGACAGAATCACTGCTGCAGCTACTCACCCCTGCATACATCACAAAGAAG
AGGGGAGAATGGAAAGATCTCATTACCACACTTGCTAAAAAGTAGGCGGC
CGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDMDPVVLSYMDSLL
RQSDVSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDHVSFISPEVTQFIK
CTSNPAEIAMFLEPLDLPNKRVVFLAINDNSNQAAGGTHWSLLVYLQDKN
SFFHYDSHSRSNSVHAKQVAEKLEAFLGRKGDKLAFVEEKAPAQQNSYDC
GMYVICNTEALCQNFFRQQTESLLQLLTPAYITKKRGEWKDLITTLAKK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20752
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial GST-Den1 wild type; ; Estimated MW of expressed protein : 51601 Daltons