cDNA Clone CDC53

• Plasmid: pYES2-GFP-CDC53
• Parent Plasmid: pYES2
• Genbank: 851424
• Tag: GFP
• Species: Saccharomyces cerevisiae

Sequence of Insert

GGATCCCTAACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGT
GCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCG
TGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAG
TTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGAC
CACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGA
AGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAG
CGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGT
GAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCG
ACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTAC
AACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAA
GGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCG
CCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTG
CCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAA
CGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGA
TCACTCTCGGCATGGACGAGCTGTACAAGGGATCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSMSETLPRSD
DLEATWNFIEPGINQILGNEKNQASTSKRVYKILSPTMYMEVYTAIYNYC
VNKSRSSGHFSTDSRTGQSTILVGSEIYEKLKNYLKNYILNFKQSNSETF
LQFYVKRWKRFTIGAIFLNHAFDYMNRYWVQKERSDGKRHIFDVNTLCLM
TWKEVMFDPSKDVLINELLDQVTLGREGQIIQRSNISTAIKSLVALGIDP
QDLKKLNLNVYIQVFEKPFLKKTQEYYTQYTNDYLEKHSVTEYIFEAHEI
IKREEKAMTIYWDDHTKKPLSMALNKVLITDHIEKLENEFVVLLDARDIE
KITSLYALIRRDFTLIPRMASVFENYVKKTGENEISSLLAMHKHNIMKNE
NANPKKLALMTAHSLSPKDYIKKLLEVHDIFSKIFNESFPDDIPLAKALD
NACGAFININEFALPAGSPKSATSKTSEMLAKYSDILLKKATKPEVASDM
SDEDIITIFKYLTDKDAFETHYRRLFAKRLIHGTSTSAEDEENIIQRLQA
ANSMEYTGKITKMFQDIRLSKILEDDFAVALKNEPDYSKAKYPDLQPFVL
AENMWPFSYQEVEFKLPKELVPSHEKLKESYSQKHNGRILKWLWPLCRGE
LKADIGKPGRMPFNFTVTLFQMAILLLYNDADVLTLENIQEGTSLTIQHI
AAAMVPFIKFKLIQQVPPGLDALVKPETQFKLSRPYKALKTNINFASGVK
NDILQSLSGGGHDNHGNKLGNKRLTEDERIEKELNTERQIFLEACIVRIM
KAKRNLPHTTLVNECIAQSHQRFNAKVSMVKRAIDSLIQKGYLQRGDDGE
SYAYLA*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU20652
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast vector for Gal1-driven expression of GFP-CDC53; Parental Plasmid SC20515 (pYES2 derived); Estimated MW of expressed protein : 121013 Daltons