cDNA Clone TRAF3

• Plasmid: pEFIRES-P-eYFP C1-TRAF3
• Parent Plasmid: pEFIRES puro eYFPC1
• Expressed: eYFP-TRAF3
• Genbank: AAH75086.1
• Tag: YFP
• Species: Human

Sequence of Insert

GGATCCATGGAGTCGAGTAAAAAGATGGACTCTCCTGGCGCGCTGCAGAC
TAACCCGCCGCTAAAGCTGCACACTGACCGCAGTGCTGGGACGCCAGTTT
TTGTCCCTGAACAAGGAGGTTACAAGGAAAAGTTTGTGAAGACCGTGGAG
GACAAGTACAAGTGTGAGAAGTGCCACCTGGTGCTGTGCAGCCCGAAGCA
GACCGAGTGTGGGCACCGCTTCTGCGAGAGCTGCATGGCGGCCCTGCTGA
GCTCTTCAAGTCCAAAATGTACAGCGTGTCAAGAGAGCATCGTTAAAGAT
AAGGTGTTTAAGGATAATTGCTGCAAGAGAGAAATTCTGGCTCTTCAGAT
CTATTGTCGGAATGAAAGCAGAGGTTGTGCAGAGCAGTTAATGCTGGGAC
ATCTGCTGGTGCATTTAAAAAATGATTGCCATTTTGAAGAACTTCCATGT
GTGCGTCCTGACTGCAAAGAAAAGGTCTTGAGGAAAGACCTGCGAGACCA
CGTGGAGAAGGCGTGTAAATACCGGGAAGCCACATGCAGCCACTGCAAGA
GTCAGGTTCCGATGATCGCGCTGCAGAAACACGAAGACACCGACTGTCCC
TGCGTGGTGGTGTCCTGCCCTCACAAGTGCAGCGTCCAGACTCTCCTGAG
GAGCGAGTTGAGTGCACACTTGTCAGAGTGTGTCAATGCCCCCAGCACCT
GTAGTTTTAAGCGCTATGGCTGCGTTTTTCAGGGGACAAACCAGCAGATC
AAGGCCCACGAGGCCAGCTCCGCCGTGCAGCACGTCAACCTGCTGAAGGA
GTGGAGCAACTCGCTCGAAAAGAAGGTTTCCTTGTTGCAGAATGAAAGTG
TAGAAAAAAACAAGAGCATACAAAGTTTGCACAATCAGATATGTAGCTTT
GAAATTGAAATTGAGAGACAAAAGGAAATGCTTCGAAATAATGAATCCAA
AATCCTTCATTTACAGCGAGTGATAGACAGCCAAGCAGAGAAACTGAAGG
AGCTTGACAAGGAGATCCGGCCCTTCCGGCAGAACTGGGAGGAAGCAGAC
AGCATGAAGAGCAGCGTGGAGTCCCTCCAGAACCGCGTGACCGAGCTGGA
GAGCGTGGACAAGAGCGCGGGGCAAGTGGCTCGGAACACAGGCCTGCTGG
AGTCCCAGCTGAGCCGGCATGACCAGATGCTGAGTGTGCACGACATCCGC
CTAGCCGACATGGACCTGCGCTTCCAGGTCCTGGAGACCGCCAGCTACAA
TGGAGTGCTCATCTGGAAGATTCGCGACTACAAGCGGCGGAAGCAGGAGG
CCGTCATGGGGAAGACCCTGTCCCTTTACAGCCAGCCTTTCTACACTGGT
TACTTTGGCTATAAGATGTGTGCCAGGGTCTACCTGAACGGGGACGGGAT
GGGGAAGGGGACGCACTTGTCGCTGTTTTTTGTCATCATGCGTGGAGAAT
ATGATGCCCTGCTTCCTTGGCCGTTTAAGCAGAAAGTGACACTCATGCTG
ATGGATCAGGGGTCCTCTCGACGTCATTTGGGAGATGCATTCAAGCCCGA
CCCCAACAGCAGCAGCTTCAAGAAGCCCACTGGAGAGATGAATATCGCCT
CTGGCTGCCCAGTCTTTGTGGCCCAAACTGTTCTAGAAAATGGGACATAT
ATTAAAGATGATACAATTTTTATTAAAGTCATAGTGGATACTTCGGATCT
GCCCGATCCCTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSMESSKK
MDSPGALQTNPPLKLHTDRSAGTPVFVPEQGGYKEKFVKTVEDKYKCEKC
HLVLCSPKQTECGHRFCESCMAALLSSSSPKCTACQESIVKDKVFKDNCC
KREILALQIYCRNESRGCAEQLMLGHLLVHLKNDCHFEELPCVRPDCKEK
VLRKDLRDHVEKACKYREATCSHCKSQVPMIALQKHEDTDCPCVVVSCPH
KCSVQTLLRSELSAHLSECVNAPSTCSFKRYGCVFQGTNQQIKAHEASSA
VQHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQSLHNQICSFEIEIERQK
EMLRNNESKILHLQRVIDSQAEKLKELDKEIRPFRQNWEEADSMKSSVES
LQNRVTELESVDKSAGQVARNTGLLESQLSRHDQMLSVHDIRLADMDLRF
QVLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCA
RVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGSSRR
HLGDAFKPDPNSSSFKKPTGEMNIASGCPVFVAQTVLENGTYIKDDTIFI
KVIVDTSDLPDP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20545
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression vector for eYFP-TRAF3. EF1a promoter, IRES and puromycin selection cassette; ; Estimated MW of expressed protein : 91965 Daltons