cDNA Clone DCUN1D3

• Plasmid: pcDNA5-FRT/TO-HA DCUN1D3
• Parent Plasmid: pcDNA5 FRT/TO HA1
• Expressed: HA-DCUN1D3
• Genbank: NM_173475*
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGGGCCAGTGTGTCACCAAGTGTAAGAATCCCTCATCGACCCT
GGGCAGCAAAAATGGAGACCGTGAGCCCAGCAACAAGTCACATAGCAGGA
GGGGTGCAGGCCACCGTGAGGAGCAGGTACCACCCTGTGGCAAGCCAGGT
GGAGATATCCTCGTCAACGGGACCAAGAAGGCCGAGGCTGCCACTGAGGC
CTGCCAGCTGCCAACGTCCTCGGGAGATGCTGGGAGGGAGTCCAAGTCCA
ATGCCGAGGAGTCTTCCTTGCAAAGATTGGAAGAACTGTTCAGGCGCTAC
AAGGATGAGCGGGAAGATGCAATTTTGGAGGAAGGCATGGAGCGCTTTTG
CAATGACCTGTGTGTTGACCCCACAGAATTTCGAGTGCTGCTCTTGGCTT
GGAAGTTCCAGGCTGCAACCATGTGCAAATTCACCAGGAAGGAGTTTTTT
GATGGCTGCAAAGCAATAAGTGCAGACAGCATTGACGGAATCTGTGCACG
GTTCCCTAGCCTCTTAACAGAAGCCAAACAAGAGGATAAATTCAAGGATC
TCTACCGGTTTACATTTCAGTTTGGCCTGGACTCTGAAGAAGGGCAGCGG
TCACTGCATCGGGAAATAGCCATTGCCCTGTGGAAACTAGTCTTTACCCA
GAACAATCCTCCGGTATTGGACCAATGGCTAAACTTCCTAACAGAGAACC
CCTCGGGGATCAAGGGCATCTCCCGGGACACTTGGAACATGTTCCTTAAC
TTCACTCAGGTGATTGGCCCTGACCTCAGCAACTACAGTGAAGATGAGGC
CTGGCCAAGTCTCTTTGACACCTTTGTGGAGTGGGAAATGGAGCGAAGGA
AAAGAGAAGGGGAAGGGAGAGGTGCACTCAGCTCAGGGCCTGAGGGCTTG
TGTCCCGAGGAGCAGACTTAGGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMGQCVTKCKNPSSTLGSKNGDREPSNKSHSRRGAGHRE
EQVPPCGKPGGDILVNGTKKAEAATEACQLPTSSGDAGRESKSNAEESSL
QRLEELFRRYKDEREDAILEEGMERFCNDLCVDPTEFRVLLLAWKFQAAT
MCKFTRKEFFDGCKAISADSIDGICARFPSLLTEAKQEDKFKDLYRFTFQ
FGLDSEEGQRSLHREIAIALWKLVFTQNNPPVLDQWLNFLTENPSGIKGI
SRDTWNMFLNFTQVIGPDLSNYSEDEAWPSLFDTFVEWEMERRKREGEGR
GALSSGPEGLCPEEQT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20466
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *carries a94c mutation (silent Arg32); mammalian Flp-In vector for expression of N-terminally HA tagged DCUN1D3; ; Estimated MW of expressed protein : 35651 Daltons