cDNA Clone Hrt1

• Plasmid: pRS413-Gal1 Pr-StrepII.6His.TUBE-Hrt1
• Parent Plasmid: pRS413
• Expressed: StrepII.6His.TUBE-Hrt1
• Genbank: 853986
• Tag: 6His
• Species: Saccharomyces cerevisiae

Sequence of Insert

GGATCCATGAGCAACGAAGTTGACAGGATGGATGTTGATGAAGATGAATCGCAAAATATTGCGCAAAGCTCAAACCAAAGTGCGCCAGTGGAAACCAAAAAGAAGAGATTTGAAATTAAGAAATGGACCGCAGTGGCGTTTTGGTCATGGGATATAGCTGTTGACAACTGTGCTATTTGCAGGAACCATATAATGGAACCATGCATTGAATGCCAGCCAAAGGCCATGACGGACACTGATAATGAATGTGTAGCAGCCTGGGGTGTCTGTAATCACGCTTTCCATTTGCACTGTATTAATAAATGGATCAAGACAAGAGACGCATGCCCATTAGATAACCAACCTTGGCAGTTAGCAAGATGCGGTAGGTGAGCGGCCGC

Amino Acid Sequence

MWSHPQFEKGAHHHHHHRSGGGVNPQLQNPEVRFQQQLEQLSAMGFLNRE
ANLQALIATGGDINAAIERLLGSQPSGGGGSGGGVNPQLQNPEVRFQQQL
EQLSAMGFLNREANLQALIATGGDINAAIERLLGSQPSGGGGSGGGVNPQ
LQNPEVRFQQQLEQLSAMGFLNREANLQALIATGGDINAAIERLLGSQPS
GGGGSGGGVNPQLQNPEVRFQQQLEQLSAMGFLNREANLQALIATGGDIN
AAIERLLGSQPSGGGGSGGGGGGGGSMSNEVDRMDVDEDESQNIAQSSNQ
SAPVETKKKRFEIKKWTAVAFWSWDIAVDNCAICRNHIMEPCIECQPKAM
TDTDNECVAAWGVCNHAFHLHCINKWIKTRDACPLDNQPWQLARCGR*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU20409
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast vector for Gal1-driven expression of Hrt1 N-terminally tagged by StrepII.6HIS.ubiquilin TUBE. ; Parental PLasmid SC20406; Estimated MW of expressed protein : 42285 Daltons