cDNA Clone DUBA-2

• Plasmid: pFBGSTDual6P-DUBA-2
• Parent Plasmid: pFastbacDual 6P1
• Expressed: GST-DUBA-2
• Genbank: NM_017602*
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGACTATACTCCCCAAAAAGAAGCCGCCGCCTCCCGACGCCGA
CCCCGCCAACGAGCCGCCGCCGCCCGGGCCGATGCCCCCGGCGCCGCGGC
GCGGCGGAGGTGTGGGCGTGGGCGGCGGCGGCACGGGCGTGGGCGGCGGC
GATCGCGACCGTGACTCCGGCGTCGTGGGGGCCCGTCCGCGAGCTTCGCC
ACCGCCTCAAGGCCCGCTACCAGGACCGCCGGGCGCTCTTCATCGCTGGG
CGCTGGCCGTGCCGCCTGGTGCAGTGGCGGGTCCCCGGCCACAACAGGCT
TCTCCACCTCCTTGCGGGGGCCCAGGTGGTCCCGGCGGCGGTCCCGGCGA
CGCGCTGGGCGCAGCGGCGGCGGGTGTGGGTGCCGCGGGCGTGGTGGTGG
GTGTGGGTGGTGCCGTAGGCGTGGGCGGCTGCTGCTCCGGGCCTGGGCAC
AGCAAGCGGCGACGTCAAGCTCCCGGGGTTGGCGCGGTTGGCGGGGGCAG
TCCCGAGCGTGAGGAGGTCGGCGCAGGCTACAACAGTGAGGACGAGTATG
AGGCGGCTGCAGCACGCATCGAGGCTATGGACCCTGCCACTGTCGAGCAG
CAGGAGCATTGGTTTGAAAAGGCCCTACGAGACAAGAAGGGCTTCATCAT
CAAGCAGATGAAGGAGGATGGCGCCTGTCTCTTCCGGGCTGTAGCTGACC
AGGTGTATGGAGACCAGGACATGCATGAGGTTGTGCGAAAGCATTGCATG
GACTATCTGATGAAGAATGCCGACTACTTCTCCAACTATGTCACAGAGGA
CTTTACCACCTACATTAACAGGAAGCGGAAAAACAATTGCCATGGCAACC
ACATTGAGATGCAGGCCATGGCAGAGATGTACAACCGTCCTGTGGAGGTG
TACCAGTACAGCACAGAACCCATCAACACATTCCATGGGATACATCAAAA
CGAGGACGAACCCATTCGTGTTAGCTACCATCGGAATATCCACTATAATT
CAGTGGTGAATCCTAACAAGGCCACCATTGGTGTGGGGCTGGGCCTGCCA
TCATTCAAACCAGGGTTTGCAGAGCAGTCTCTGATGAAGAATGCCATAAA
AACATCGGAGGAGTCATGGATTGAACAGCAGATGCTAGAAGACAAGAAAC
GGGCCACAGACTGGGAGGCCACAAATGAAGCCATCGAGGAGCAGGTGGCT
CGGGAATCCTACCTGCAGTGGTTGCGGGATCAGGAGAAACAGGCTCGCCA
GGTCCGAGGCCCCAGCCAGCCCCGGAAAGCCAGCGCCACATGCAGTTCGG
CCACAGCAGCAGCCTCCAGTGGCCTGGAGGAGTGGACTAGCCGGTCCCCG
CGGCAGCGGAGTTCAGCCTCGTCACCTGAGCACCCTGAGCTGCATGCTGA
ATTGGGCATGAAGCCCCCTTCCCCAGGCACTGTTTTAGCTCTTGCCAAAC
CTCCTTCGCCCTGTGCGCCAGGTACAAGCAGTCAGTTCTCGGCAGGGGCC
GACCGGGCAACTTCCCCCCTTGTGTCCCTCTACCCTGCTTTGGAGTGCCG
GGCCCTCATTCAGCAGATGTCCCCCTCTGCCTTTGGTCTGAATGACTGGG
ATGATGATGAGATCCTAGCTTCGGTGCTGGCAGTGTCCCAACAGGAATAC
CTAGACAGTATGAAGAAAAACAAAGTGCCCTGCAGGTGTTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMTILPKKKPPPPDADPANEPPPPGPMPPAPRRGGGVGVGGGGTGVGGGDRDRDSGVVGARPRASPPPQGPLPGPPGALHRWALAVPPGAVAGPRPQQASPPPCGGPGGPGGGPGDALGAAAAGVGAAGVVVGVGGAVGVGGCCSGPGHSKRRRQAPGVGAVGGGSPEREEVGAGYNSEDEYEAAAARIEAMDPATVEQQEHWFEKALRDKKGFIIKQMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMAEMYNRPVEVYQYSTEPINTFHGIHQNEDEPIRVSYHRNIHYNSVVNPNKATIGVGLGLPSFKPGFAEQSLMKNAIKTSEESWIEQQMLEDKKRATDWEATNEAIEEQVARESYLQWLRDQEKQARQVRGPSQPRKASATCSSATAAASSGLEEWTSRSPRQRSSASSPEHPELHAELGMKPPSPGTVLALAKPPSPCAPGTSSQFSAGADRATSPLVSLYPALECRALIQQMSPSAFGLNDWDDDEILASVLAVSQQEYLDSMKKNKVPCRC*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU20025
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus construct to express DUBA-2 with N-terminal GST tag (contains PreScission protease site); ; Estimated MW of expressed protein : 86465 Daltons