cDNA Clone DUBA-2

• Plasmid: pFBHT-DUBA-2
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-DUBA-2
• Genbank: NM_017602*
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGACTATACTCCCCAAAAAGAAGCCGCCGCCTCCCGACGCCGA
CCCCGCCAACGAGCCGCCGCCGCCCGGGCCGATGCCCCCGGCGCCGCGGC
GCGGCGGAGGTGTGGGCGTGGGCGGCGGCGGCACGGGCGTGGGCGGCGGC
GATCGCGACCGTGACTCCGGCGTCGTGGGGGCCCGTCCGCGAGCTTCGCC
ACCGCCTCAAGGCCCGCTACCAGGACCGCCGGGCGCTCTTCATCGCTGGG
CGCTGGCCGTGCCGCCTGGTGCAGTGGCGGGTCCCCGGCCACAACAGGCT
TCTCCACCTCCTTGCGGGGGCCCAGGTGGTCCCGGCGGCGGTCCCGGCGA
CGCGCTGGGCGCAGCGGCGGCGGGTGTGGGTGCCGCGGGCGTGGTGGTGG
GTGTGGGTGGTGCCGTAGGCGTGGGCGGCTGCTGCTCCGGGCCTGGGCAC
AGCAAGCGGCGACGTCAAGCTCCCGGGGTTGGCGCGGTTGGCGGGGGCAG
TCCCGAGCGTGAGGAGGTCGGCGCAGGCTACAACAGTGAGGACGAGTATG
AGGCGGCTGCAGCACGCATCGAGGCTATGGACCCTGCCACTGTCGAGCAG
CAGGAGCATTGGTTTGAAAAGGCCCTACGAGACAAGAAGGGCTTCATCAT
CAAGCAGATGAAGGAGGATGGCGCCTGTCTCTTCCGGGCTGTAGCTGACC
AGGTGTATGGAGACCAGGACATGCATGAGGTTGTGCGAAAGCATTGCATG
GACTATCTGATGAAGAATGCCGACTACTTCTCCAACTATGTCACAGAGGA
CTTTACCACCTACATTAACAGGAAGCGGAAAAACAATTGCCATGGCAACC
ACATTGAGATGCAGGCCATGGCAGAGATGTACAACCGTCCTGTGGAGGTG
TACCAGTACAGCACAGAACCCATCAACACATTCCATGGGATACATCAAAA
CGAGGACGAACCCATTCGTGTTAGCTACCATCGGAATATCCACTATAATT
CAGTGGTGAATCCTAACAAGGCCACCATTGGTGTGGGGCTGGGCCTGCCA
TCATTCAAACCAGGGTTTGCAGAGCAGTCTCTGATGAAGAATGCCATAAA
AACATCGGAGGAGTCATGGATTGAACAGCAGATGCTAGAAGACAAGAAAC
GGGCCACAGACTGGGAGGCCACAAATGAAGCCATCGAGGAGCAGGTGGCT
CGGGAATCCTACCTGCAGTGGTTGCGGGATCAGGAGAAACAGGCTCGCCA
GGTCCGAGGCCCCAGCCAGCCCCGGAAAGCCAGCGCCACATGCAGTTCGG
CCACAGCAGCAGCCTCCAGTGGCCTGGAGGAGTGGACTAGCCGGTCCCCG
CGGCAGCGGAGTTCAGCCTCGTCACCTGAGCACCCTGAGCTGCATGCTGA
ATTGGGCATGAAGCCCCCTTCCCCAGGCACTGTTTTAGCTCTTGCCAAAC
CTCCTTCGCCCTGTGCGCCAGGTACAAGCAGTCAGTTCTCGGCAGGGGCC
GACCGGGCAACTTCCCCCCTTGTGTCCCTCTACCCTGCTTTGGAGTGCCG
GGCCCTCATTCAGCAGATGTCCCCCTCTGCCTTTGGTCTGAATGACTGGG
ATGATGATGAGATCCTAGCTTCGGTGCTGGCAGTGTCCCAACAGGAATAC
CTAGACAGTATGAAGAAAAACAAAGTGCCCTGCAGGTGTTGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMTILPKKKPPPPDADPANEPPPPGPMPPAPRRGGGVGVGGGGTGVGGGDRDRDSGVVGARPRASPPPQGPLPGPPGALHRWALAVPPGAVAGPRPQQASPPPCGGPGGPGGGPGDALGAAAAGVGAAGVVVGVGGAVGVGGCCSGPGHSKRRRQAPGVGAVGGGSPEREEVGAGYNSEDEYEAAAARIEAMDPATVEQQEHWFEKALRDKKGFIIKQMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMAEMYNRPVEVYQYSTEPINTFHGIHQNEDEPIRVSYHRNIHYNSVVNPNKATIGVGLGLPSFKPGFAEQSLMKNAIKTSEESWIEQQMLEDKKRATDWEATNEAIEEQVARESYLQWLRDQEKQARQVRGPSQPRKASATCSSATAAASSGLEEWTSRSPRQRSSASSPEHPELHAELGMKPPSPGTVLALAKPPSPCAPGTSSQFSAGADRATSPLVSLYPALECRALIQQMSPSAFGLNDWDDDEILASVLAVSQQEYLDSMKKNKVPCRC*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU20023
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus construct to express DUBA-2 with N-terminal 6HIS tag (contains TEV protease site); With reference to NM_017602 DUBA-2 has a deletion spanning nt910-924. From nt1687 the 3’ end diverges from the reference sequence. Catalytic residues in this clone are Asp221, Cys224 and His329.; ; Estimated MW of expressed proteins : 63012 Daltons