cDNA Clone Lag2

• Plasmid: pYES2-Lag2-GFP
• Parent Plasmid: pYES2
• Expressed: Lag2-GFP
• Tag: GFP

Sequence of Insert

ggtaccCTAACCATGAGCTTGCACATTAGTAAACTGATTGAGCAATATCGTTCCACTAAGGACAATGACCTTAAATATATGCTTTTAAGGCAAAACTTTAAAATTAACGACATAGAGGATGAGCTGGCTCCTCTTGTTAACGAATTGCTGCTCCCGGTATTGGTCGAAGAACAAGACATGGAAATTCTCAACCTAGTATCGTTTCAAGTGCTCCCCGATTTAGTTCTTTCTATGATCAGCGACCCTGCAGCTGCTCAGTTGGGATGGGTAATAAGCTTGATATGCGACCCTTTATTAAACCAAAGCATGATACATGCAAATCGTTCTTTTGTTCTGATTGAGACACTAAGAAATGTGTTACAAAAAATTGAAAACTCCCCTCATTTAGATTACCACCAACCAGTAAACAGTTCATTAGAGTTTATCTCCAAATTTATTGTTGAAATGAAAAGGCATATGTGTGATGTGGATGCTGCGCAGCTTTCACATTCACTTTCTGAAAGCAATATGCTAATTTACATAGAATCCCTTAATCTATTGCTAAAATTTAGCTTCTTTTCAGACGCGGCAAGTCCTTCAGTAATGGTCACACTGCCCTTTGATATTTTGAATGACGTTTTTACAATTGCGCAGGACTATTCTGCTACGAATACAAATGAAAGCATTGATAGGATCACAGAAAAATTACTTTTGACTTCTACGCAATTAACTCATCCGGTGGACCTGGAAAATTTATGCCCCAAAATGAAGTACAATACTTTAGCAGCAGTTTCTCGAATATGGTACAAATTTGGCCCCATAGTCGATAAATTATTTACTAACCGGCTATTACCGGTTCTATTCCCCCCGCAAATGGGAGAAGAATGTAACGTCGAAGATGTTCTCGAAATAGTTCATAACTTCCATCCATATTTTTCTATTCGTAGGCTAAAGGATAATAGGCCTCTATTGTCAGATTCGACTATTAGCCAGCTAAGAGAGGGTTTGTTTGGTATGCTCAGCATATTAAACGACTCGCTGACAAGGACGCAAAATGAAAACGACCATGGTAGCGACAATCTGATCGACAGTGACGACGGATTTGGCTCAGACAACGATCCTGAACAACAAGCGTACCTCGATGAACTCGTATCAGAAGGTTATGACGAAAACATGTATGACGGTGATACGGATGACGAAGACGCAGATGATATAAACGTTGAAAAAAATGATGAAGCCACCAAAGACATTACAGAAACGAACAAAATCCTTTTAATCTTTTCTGAGTTACATTATCCTCAGGAGGAGCGCTTCTCTGAACTGTTGGTTGAGTTGCAGACTAAGATCGCTATAAACACATCCTTAATTGATAAAATTCTTTCAAAAGAGACAACAGAATTACCCACTCACAATGGGGAAATCGCAGATTTGAACGAAATTCTAAATGAAGTGAAAGGCAATAAACCAATTAGAAAAAACGTTATTTTTTGTACGTTAGCGCATACTTTGTCGTTGCAATCCGGTTCCGAATTGTCTGTTCTACAGCTATCAATAGAGGTTATTGATCATCTGCTGGTTAAAAATCATTCAAATAACATCACTCGTGGCGAGCAATTTCAATTGATTAAGTTGATATTACCCCATCTAAAAACAAATAAATCTTTTATTGATACATTAAAAGCAGGAAATTTTACCCAAAAAATTGATGAAGGAGTGACCTTAAGAACAATGATTCTTTCTCTACTACTACAACTATTCCCACTGGATTACTCTATGCTAGGTGAGATTTTACCCACTATTGCGCGATATTCTGTAAGGGATAAGGATCTTGGGGTGAGAGATCTGTCCTTCCAATTGCTGGACCAAATTCTACGCACATACTATAATTACCTAATTGGTATCGATTGGGAATGGTATAAAGATGACTTCTATCAAGTTTTGCAAGAAACTTGTATTAAAAAAGACATAAATACCAACCTCTTGCTACAATTTCCTCCTTATCTCCCTCATGACggatccATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGtgagcggccgc

Amino Acid Sequence

MSLHISKLIEQYRSTKDNDLKYMLLRQNFKINDIEDELAPLVNELLLPVLVEEQDMEILNLVSFQVLPDLVLSMISDPAAAQLGWVISLICDPLLNQSMIHANRSFVLIETLRNVLQKIENSPHLDYHQPVNSSLEFISKFIVEMKRHMCDVDAAQLSHSLSESNMLIYIESLNLLLKFSFFSDAASPSVMVTLPFDILNDVFTIAQDYSATNTNESIDRITEKLLLTSTQLTHPVDLENLCPKMKYNTLAAVSRIWYKFGPIVDKLFTNRLLPVLFPPQMGEECNVEDVLEIVHNFHPYFSIRRLKDNRPLLSDSTISQLREGLFGMLSILNDSLTRTQNENDHGSDNLIDSDDGFGSDNDPEQQAYLDELVSEGYDENMYDGDTDDEDADDINVEKNDEATKDITETNKILLIFSELHYPQEERFSELLVELQTKIAINTSLIDKILSKETTELPTHNGEIADLNEILNEVKGNKPIRKNVIFCTLAHTLSLQSGSELSVLQLSIEVIDHLLVKNHSNNITRGEQFQLIKLILPHLKTNKSFIDTLKAGNFTQKIDEGVTLRTMILSLLLQLFPLDYSMLGEILPTIARYSVRDKDLGVRDLSFQLLDQILRTYYNYLIGIDWEWYKDDFYQVLQETCIKKDINTNLLLQFPPYLPHDGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU23201
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: inc yeast inititation, GFP has c117a silent mutation (nt)