cDNA Clone NEK5

• Plasmid: pFastbac GST NEK5
• Parent Plasmid: undefined
• Expressed: GST-3C-NEK5
• Genbank: NM_199289.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGATAAGTACGATGTGATTAAGGCCATCGGGCAAGGTGCCTTCGGGAAAGCATACTTAGCTAAAGGGAAATCAGATAGCAAGCACTGTGTCATAAAAGAGATCAATTTTGAAAAGATGCCCATACAAGAAAAAGAAGCTTCAAAGAAAGAAGTGATTCTTCTGGAAAAGATGAAACATCCCAACATTGTAGCCTTCTTCAATTCATTTCAAGAGAATGGCAGGCTGTTTATTGTAATGGAATATTGTGATGGAGGGGATCTCATGAAAAGGATCAATAGACAACGGGGTGTGTTATTTAGTGAAGATCAGATCCTCGGTTGGTTTGTACAGATTTCTCTAGGACTAAAACATATTCATGACAGGAAGATATTACACAGGGACATAAAAGCTCAGAACATTTTTCTTAGCAAGAACGGAATGGTGGCAAAGCTTGGGGACTTTGGTATAGCAAGAGTCCTGAATAATTCCATGGAACTTGCTCGAACTTGTATTGGAACACCTTACTACCTGTCCCCAGAGATCTGTCAGAATAAACCCTACAACAATAAAACGGATATTTGGTCTCTTGGCTGTGTCTTATATGAGCTCTGCACACTTAAACATCCTTTTGAGGGTAACAACTTACAGCAGCTGGTTCTGAAGATTTGTCAAGCACATTTTGCCCCAATATCTCCGGGGTTTTCTCGTGAGCTCCATTCCTTGATATCTCAGCTCTTTCAAGTATCTCCTCGAGACCGACCATCCATAAATTCCATTTTGAAAAGGCCCTTTTTAGAGAATCTTATTCCCAAATATTTGACTCCTGAGGTCATTCAGGAAGAATTCAGTCACATGCTTATATGCAGAGCAGGAGCGCCAGCTTCTCGACATGCTGGGAAGGTGGTCCAGAAGTGTAAAATACAAAAAGTGAGATTCCAGGGAAAGTGCCCACCAAGATCAAGGATATCTGTGCCAATTAAAAGGAATGCTATATTGCATAGAAATGAATGGAGACCACCAGCTGGAGCCCAGAAGGCCAGATCTATAAAAATGATAGAAAGACCCAAAATTGCTGCTGTCTGTGGACATTATGATTATTATTATGCTCAACTTGATATGCTGAGGAGGAGAGCCCACAAACCAAGTTATCACCCTATTCCTCAAGAAAATACTGGAGTTGAGGATTACGGTCAGGAAACGAGGCATGGTCCATCCCCAAGTCAATGGCCTGCTGAGTACCTTCAGAGAAAATTTGAAGCTCAACAATATAAGTTGAAAGTGGAGAAGCAATTGGGTCTTCGTCCATCTTCTGCCGAGCCAAATTACAACCAGAGACAAGAGCTAAGAAGTAATGGAGAAGAGCCTAGATTCCAGGAGCTGCCATTTAGGAAAAACGAAATGAAGGAACAGGAATATTGGAAGCAGTTAGAGGAAATACGCCAACAGTACCACAATGACATGAAAGAAATTAGAAAGAAGATGGGGAGAGAACCAGAGGAGAACTCAAAAATAAGTCATAAAACCTATTTGGTGAAGAAGAGTAACCTGCCTGTCCATCAAGATGCATCTGAGGGAGAAGCACCTGTGCAGATGGAATTTCGCTCTTGTTGCCCAGGCTGGAGTGCAATGGCACGATCTTGGCTCACCGCAACCTCCGCCTCCCAGGACATTGAAAAAGACTTGAAACAAATGAGGCTTCAGAACACAAAGGAAAGTAAAAATCCAGAACAGAAATATAAAGCTAAGAAGGGGGTAAAATTTGAAATTAATTTAGACAAATGTATTTCTGATGAAAACATCCTCCAAGAGGAAGAGGCAATGGATATACCAAATGAAACTTTGACCTTTGAGGATGGCATGAAGTTTAAGGAATATGAATGTGTAAAGGAGCATGGAGATTATACAGACAAAGCATTTGAAAAACTTCACTGCCCAGAAGCAGGGTTTTCCACGCAGACTGTAGCTGCTGTGGGAAACAGGAGGCAGTGGGATGGAGGAGCGCCTCAGACTCTGCTGCAGATGATGGCAGTGGCCGACATCACCTCCACCTGCCCCACGGGGCCTGACAGTGAGTCTGTGCTTAGCGTCAGTCGTCAGGAAGGGAAGACCAAGGACCCGTACAGCCCAGTGCTCATCCTGATGTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMDKYDVIKAIGQGAFGKAYLAKGKSDSKHCVIKEINFEKMPIQEKEASKKEVILLEKMKHPNIVAFFNS
FQENGRLFIVMEYCDGGDLMKRINRQRGVLFSEDQILGWFVQISLGLKHIHDRKILHRDIKAQNIFLSKNGMVAKLGDFGIARVLNNSMELARTCIGTPY
YLSPEICQNKPYNNKTDIWSLGCVLYELCTLKHPFEGNNLQQLVLKICQAHFAPISPGFSRELHSLISQLFQVSPRDRPSINSILKRPFLENLIPKYLTP
EVIQEEFSHMLICRAGAPASRHAGKVVQKCKIQKVRFQGKCPPRSRISVPIKRNAILHRNEWRPPAGAQKARSIKMIERPKIAAVCGHYDYYYAQLDMLR
RRAHKPSYHPIPQENTGVEDYGQETRHGPSPSQWPAEYLQRKFEAQQYKLKVEKQLGLRPSSAEPNYNQRQELRSNGEEPRFQELPFRKNEMKEQEYWKQ
LEEIRQQYHNDMKEIRKKMGREPEENSKISHKTYLVKKSNLPVHQDASEGEAPVQMEFRSCCPGWSAMARSWLTATSASQDIEKDLKQMRLQNTKESKNP
EQKYKAKKGVKFEINLDKCISDENILQEEEAMDIPNETLTFEDGMKFKEYECVKEHGDYTDKAFEKLHCPEAGFSTQTVAAVGNRRQWDGGAPQTLLQMM
AVADITSTCPTGPDSESVLSVSRQEGKTKDPYSPVLILM*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pFastbac GST NEK595.08 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU41230
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No