cDNA Clone KLHL3

• Plasmid: pCMV-FLAG-1 KLHL3 A77E
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG-KLHL3 A77E
• Genbank: NM_017415.2
• Tag: FLAG
• Species: Human

Sequence of Insert

AGATCTatggagggtgaaagtgtcaagctgagctcccagactctgataca
ggctggggatgatgagaagaaccagaggacgatcactgtcaaccctgccc
acatggggaaagcattcaaggttatgaatgaactgcggagtaaacagctg
ttgtgtgacgtgatgattgtggcagaagatgtcgagatagaagcccaccg
tgtggtcctggcagcctgcagcccctacttctgtgAgatgttcacaggtg
acatgtctgagagtaaagccaaaaagatagaaatcaaggacgtggatggg
cagacgctgagtaagctgattgactacatctatactgctgaaatcgaggt
gactgaagagaatgtccaggtgctgctcccggcagccagcttgctgcagc
tcatggatgttcggcagaactgctgtgacttcctgcagtctcagttgcat
cccaccaattgcctgggcatccgtgcgtttgcagatgtacacacctgcac
tgaccttctgcagcaggccaatgcctacgcagagcagcactttccagagg
tgatgctaggagaagaatttcttagcctgagtctggaccaggtgtgcagc
ttgatatccagcgacaagctgaccgtttcttcagaagagaaggtgtttga
agctgtgatctcatggatcaattatgagaaagaaacccgtttagagcaca
tggcaaagctgatggaacatgtccgacttcctctcttacctagggactac
ctagtccaaacggttgaagaagaagctttgataaagaataacaacacctg
taaagacttcctcattgaggccatgaaataccatctcctccctctggatc
agagactattgattaagaacccaaggaccaagcccaggactccagtcagc
cttcccaaggtcatgattgtggttggcggccaggcacccaaggcaatccg
cagtgtggagtgctatgatttcgaggaggaccggtgggatcagattgctg
agcttccttccagaagatgcagagcaggtgtggtgttcatggctggccac
gtgtatgccgtgggagggtttaatggctcactgcgggtgcggacagtgga
tgtgtatgacggcgtgaaggaccagtggacgtccattgccagcatgcagg
agcgccggagcacactgggcgcagcggtgctcaatgacttgctctacgca
gtgggaggctttgatggcagtactggcctagcatcggtggaagcctacag
ctacaagaccaacgagtggttctttgtggccccgatgaacacgcggcgga
gcagtgtgggtgtgggcgttgtggaggggaagctatatgctgttgggggt
tatgatggagcttcccgccagtgtctgagcactgtggagcagtacaaccc
agcgaccaatgaatggatatacgtggcggacatgagcacccgccgcagtg
gcgcaggggttggagtgcttagcggacagctgtacgccacaggtgggcat
gatgggcctttggtgaggaagagcgttgaggtttacgatcctggaacaaa
tacctggaagcaagtggcagacatgaacatgtgccggcgcaacgcagggg
tctgtgcagtaaatgggctcctgtatgtggttggaggggatgatggatcc
tgcaacttggcttcggtggagtactacaatcctgtcactgacaaatggac
gctgcttccaacgaacatgagcacggggcggagctatgcaggtgttgccg
tgattcacaagtccttgtgaGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMEGESVKLSSQTLIQAGDDEKNQRTITVNPAHMGKAFKV
MNELRSKQLLCDVMIVAEDVEIEAHRVVLAACSPYFCEMFTGDMSESKAK
KIEIKDVDGQTLSKLIDYIYTAEIEVTEENVQVLLPAASLLQLMDVRQNC
CDFLQSQLHPTNCLGIRAFADVHTCTDLLQQANAYAEQHFPEVMLGEEFL
SLSLDQVCSLISSDKLTVSSEEKVFEAVISWINYEKETRLEHMAKLMEHV
RLPLLPRDYLVQTVEEEALIKNNNTCKDFLIEAMKYHLLPLDQRLLIKNP
RTKPRTPVSLPKVMIVVGGQAPKAIRSVECYDFEEDRWDQIAELPSRRCR
AGVVFMAGHVYAVGGFNGSLRVRTVDVYDGVKDQWTSIASMQERRSTLGA
AVLNDLLYAVGGFDGSTGLASVEAYSYKTNEWFFVAPMNTRRSSVGVGVV
EGKLYAVGGYDGASRQCLSTVEQYNPATNEWIYVADMSTRRSGAGVGVLS
GQLYATGGHDGPLVRKSVEVYDPGTNTWKQVADMNMCRRNAGVCAVNGLL
YVVGGDDGSCNLASVEYYNPVTDKWTLLPTNMSTGRSYAGVAVIHKSL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU23144
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: clone harbours silent a471g substitution (Ala157)