cDNA Clone HPK1

• Synonyms: mitogen-activated protein kinase kinase kinase kinase 1 isoform 1, MAP4K1
• Plasmid: pRETROX Tight HA HPK1
• Parent Plasmid: pRetroX tight puro
• Expressed: HA
• Genbank: NP_001036065.1
• Tag: HA
• Species: Human

Sequence of Insert

GGCGGCCGCGGCCACCATGTACCCATACGATGTGCCAGATTACGCCatgg acgtcgtggaccctgacattttcaatagagacccccgggaccactatgac ctgctacagcggctgggtggcggcacgtatggggaagtctttaaggctcg agacaaggtgtcaggggacctggtggcactgaagatggtgaagatggagc ctgatgatgatgtctccacccttcagaaggaaatcctcatattgaaaact tgccggcacgccaacatcgtggcctaccatgggagttatctctggttgca gaaactctggatctgcatggaattctgtggggctggttctctccaggaca tctaccaagtgacaggctccctgtcagagctccagattagctatgtctgc cgggaagtgctccagggactggcctatttgcactcacagaagaagataca cagggacatcaagggagctaacatcctcatcaatgatgctggggaggtca gattggctgactttggcatctcggcccagattggggctacactggccaga cgcctctctttcattgggacaccctactggatggctccggaagtggcagc tgtggccctgaagggaggatacaatgagctgtgtgacatctggtccctgg gcatcacggccatcgaactggccgagctacagccaccgctctttgatgtg caccctctcagagttctcttcctcatgaccaagagtggctaccagcctcc ccgactgaaggaaaaaggcaaatggtcggctgccttccacaacttcatca aagtcactctgactaagagtcccaagaaacgacccagcgccaccaagatg ctcagtcatcaactggtatcccagcctgggctgaatcgaggcctgatcct ggatcttcttgacaaactgaagaatcccgggaaaggaccctccattgggg acattgaggatgaggagcccgagctaccccctgctatccctcggcggatc agatccacccaccgctccagctctctggggatcccagatgcagactgctg tcggcggcacatggagttcaggaagctccgaggaatggagaccagacccc cagccaacaccgctcgcctacagcctcctcgagacctcaggagcagcagc cccaggaagcaactgtcagagtcgtctgacgatgactatgacgacgtgga catccccacccctgcagaggacacacctcctccacttccccccaagccca agttccgttctccatcagacgagggtcctgggagcatgggggatgatggg cagctgagcccgggggtgctggtccggtgtgccagtgggcccccaccaaa cagcccccgtcctgggcctcccccatccaccagcagcccccacctcaccg cccattcagaaccctcactctggaacccaccctcccgggagcttgacaag cccccacttctgccccccaagaaggaaaagatgaagagaaagggatgtgc ccttctcgtaaagttgttcaatggctgccccctccggatccacagcacgg ccgcctggacacatccctccaccaaggaccagcacctgctcctgggggca gaggaaggcatctttatcctgaaccggaatgaccaggaggccacgctgga aatgctctttcctagccggactacgtgggtgtactccatcaacaacgttc tcatgtctctctcaggaaagaccccccacctgtattctcatagcatcctt ggcctgctggaacggaaagagaccagagcaggaaaccccatcgctcacat tagcccccaccgcctactggcaaggaagaacatggtttccaccaagatcc aggacaccaaaggctgccgggcgtgctgtgtggcggagggtgcgagctct gggggcccgttcctgtgcggtgcattggagacgtccgttgtcctgcttca gtggtaccagcccatgaacaaattcctgcttgtccggcaggtgctgttcc cactgccgacgcctctgtccgtgttcgcgctgctgaccgggccaggctct gagctgcccgctgtgtgcatcggcgtgagccccgggcggccggggaagtc ggtgctcttccacacggtgcgctttggcgcgctctcttgctggctgggcg agatgagcaccgagcacaggggacccgtgcaggtgacccaggtagaggaa gatatggtgatggtgttgatggatggctctgtgaagctggtgaccccgga ggggtccccagtccggggacttcgcacacctgagatccccatgaccgaag cggtggaggccgtggctatggttggaggtcagcttcaggccttctggaag catggagtgcaggtgtgggctctaggctcggatcagctgctacaggagct gagagaccctaccctcactttccgtctgcttggctcccccaggcctgtag tggtggagacacgcccagtggatgatcctactgctcccagcaacctctac atccaggaatgagcggccgc

Amino Acid Sequence

MYPYDVPDYAMDVVDPDIFNRDPRDHYDLLQRLGGGTYGEVFKARDKVSG
DLVALKMVKMEPDDDVSTLQKEILILKTCRHANIVAYHGSYLWLQKLWIC
MEFCGAGSLQDIYQVTGSLSELQISYVCREVLQGLAYLHSQKKIHRDIKG
ANILINDAGEVRLADFGISAQIGATLARRLSFIGTPYWMAPEVAAVALKG
GYNELCDIWSLGITAIELAELQPPLFDVHPLRVLFLMTKSGYQPPRLKEK
GKWSAAFHNFIKVTLTKSPKKRPSATKMLSHQLVSQPGLNRGLILDLLDK
LKNPGKGPSIGDIEDEEPELPPAIPRRIRSTHRSSSLGIPDADCCRRHME
FRKLRGMETRPPANTARLQPPRDLRSSSPRKQLSESSDDDYDDVDIPTPA
EDTPPPLPPKPKFRSPSDEGPGSMGDDGQLSPGVLVRCASGPPPNSPRPG
PPPSTSSPHLTAHSEPSLWNPPSRELDKPPLLPPKKEKMKRKGCALLVKL
FNGCPLRIHSTAAWTHPSTKDQHLLLGAEEGIFILNRNDQEATLEMLFPS
RTTWVYSINNVLMSLSGKTPHLYSHSILGLLERKETRAGNPIAHISPHRL
LARKNMVSTKIQDTKGCRACCVAEGASSGGPFLCGALETSVVLLQWYQPM
NKFLLVRQVLFPLPTPLSVFALLTGPGSELPAVCIGVSPGRPGKSVLFHT
VRFGALSCWLGEMSTEHRGPVQVTQVEEDMVMVLMDGSVKLVTPEGSPVR
GLRTPEIPMTEAVEAVAMVGGQLQAFWKHGVQVWALGSDQLLQELRDPTL
TFRLLGSPRPVVVETRPVDDPTAPSNLYIQE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU43587
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: please clone insert of DU39694 into pRETRO-X-Tight