cDNA Clone Lys-C

• Synonyms: Lysyl endopeptidase
• Plasmid: pET6HIS SUMO LYS-C + linker
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6HIS SUMO LYS-C + linker
• Genbank: Q7M135
• Tag: SUMO
• Species: Lysobacter enzymogenes

Sequence of Insert

GGATCCGGTGTTAGCGGTAGCTGTAATATTGATGTTGTTTGTCCGGAAGGTAATGGTCATCGTGATGTTATTCGTAGCGTTGCAGCATATAGCAAACAGGGCACCATGTGGTGTACCGGTAGCCTGGTGAATAATAGCGCAAATGACAAAAAAATGTACTTTCTGACCGCCAATCATTGTGGTATGACCACCGCAGCAATTGCAAGCAGCATGGTTGTTTATTGGAATTATCAGAATAGCACCTGTCGTGCACCGGGTAGCAGCAGCAGCGGTGCAAATGGTGATGGTAGTCTGGCACAGAGCCAGACCGGTGCAGTTGTTCGTGCAACCAATGCAGCAAGCGATTTTACCCTGCTGGAACTGAATACCGCAGCCAATCCGGCATATAACCTGTTTTGGGCAGGTTGGGATCGTCGTGATCAGAATTTTGCCGGTGCAACCGCAATTCATCATCCGAATGTTGCAGAAAAACGTATTAGCCATAGCACCGTTGCAACCGAAATTAGCGGTTATAATGGTGCCACCGGCACCAGCCATCTGCATGTTTTTTGGCAGGCAAGCGGTGGTGTTACCGAACCGGGTTCAAGCGGTAGCCCGATTTATAGTCCGGAAAAACGTGTTCTGGGTCAGCTGCATGGTGGTCCGAGCAGCTGTAGCGCAACCGGTGCCGATCGTAGCGATTATTATGGTCGTGTTTTTACCAGCTGGACCGGTGGTGGTACAAGCGCAACCCGTCTGAGCGATTGGCTGGATGCAGCAGGTACAGGTGCACAGTTTATTGATGGTCTGGATAGTACCGGCACCCCTCCGGTTTAAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGSGVSGSCNIDVVCPEGNGHRDVIRSVAAYSKQGTMWCTGSLVNNSANDKKMYFLTANHCGMTTAAIASSMVVYWNYQNSTCRAPGSSSSGANGDGSLAQSQTGAVVRATNAASDFTLLELNTAANPAYNLFWAGWDRRDQNFAGATAIHHPNVAEKRISHSTVATEISGYNGATGTSHLHVFWQASGGVTEPGSSGSPIYSPEKRVLGQLHGGPSSCSATGADRSDYYGRVFTSWTGGGTSATRLSDWLDAAGTGAQFIDGLDSTGTPPV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU43083
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee