cDNA Clone OSR1

• Plasmid: pCMV HALO P OSR1 D459A
• Parent Plasmid: pCMV5 Halo 3C1
• Expressed: HALO P OSR1 D459A
• Tag: HALO
• Species: Human

Sequence of Insert

GGATCCATGTCCGAAGACTCGAGCGCCCTGCCCTGGTCCATCAACAGGGACGATTACGAGCTGCAGGAGGTGATCGGGAGTGGAGCAACTGCTGTAGTCC
AAGCAGCTTATTGTGCCCCTAAAAAGGAGAAAGTGGCAATCAAACGGATAAACCTTGAGAAATGTCAAACTAGCATGGATGAACTCCTGAAAGAAATTCA
AGCCATGAGTCAATGCCATCATCCTAATATTGTATCTTACTACACATCTTTTGTGGTAAAAGATGAGCTGTGGCTTGTCATGAAGCTGCTAAGTGGAGGT
TCTGTTCTGGATATTATTAAGCACATTGTGGCAAAAGGGGAACACAAAAGTGGAGTCCTAGATGAATCTACCATTGCTACGATACTCCGAGAAGTACTGG
AAGGGCTGGAATATCTGCATAAAAATGGACAGATCCACAGAGATGTGAAAGCTGGAAACATTCTTCTTGGAGAAGATGGCTCAGTACAGATTGCAGACTT
TGGGGTTAGTGCTTTTTTAGCAACTGGTGGTGATATTACCCGAAATAAAGTGAGAAAGACCTTTGTTGGCACCCCTTGTTGGATGGCACCTGAAGTTATG
GAACAGGTCCGTGGTTATGATTTCAAAGCTGATATTTGGAGTTTTGGAATTACAGCAATTGAATTGGCTACAGGGGCGGCTCCTTATCATAAATATCCAC
CAATGAAGGTTTTAATGCTGACACTGCAGAACGATCCTCCTTCTTTGGAAACTGGTGTTCAAGATAAAGAAATGCTGAAAAAATATGGAAAATCATTTAG
AAAAATGATTTCATTGTGCCTTCAAAAAGATCCAGAAAAAAGACCAACAGCAGCAGAACTATTAAGGCACAAATTTTTCCAGAAAGCAAAGAATAAAGAA
TTTCTTCAAGAAAAAACATTGCAGAGAGCACCAACCATTTCTGAAAGAGCAAAAAAGGTTCGGAGGGTACCAGGTTCCAGTGGGCGTCTTCATAAGACAG
AGGATGGAGGCTGGGAGTGGAGTGATGATGAATTTGATGAAGAAAGTGAGGAAGGGAAAGCAGCAATTTCACAACTCAGGTCTCCCCTAGTGAAAGAATC
AATATCAAATTCTGAGCTCTTTCCAACAACTGATCCTGTGGGTACTTTGCTCCAAGTTCCAGAACAGATCTCTGCTCATCTACCTCAGCCAGCTGGGCAG
ATTGCTACACAGCCAACTCAAGTCTCTCTCCCACCCACCGCAGAGCCAGCAAAAACAGCTCAGGCTTTGTCTTCAGGATCAGGTTCACAAGAAACCAAGA
TCCCAATCAGTCTAGTACTAAGATTAAGGAATTCCAAAAAAGAACTAAATGATATTCGATTTGAATTTACTCCTGGGAGAGCTACAGCAGAGGGTGTCTC
TCAGGAACTCATTTCTGCTGGCCTGGTCGACGGAAGGGATTTAGTAATAGTGGCAGCTAATTTGCAGAAAATTGTGGAAGAACCTCAGTCAAATCGATCT
GTCACTTTCAAACTGGCGTCTGGTGTCGAAGGCTCAGATATTCCTGATGATGGTAAACTGATAGGATTTGCCCAGCTCAGCATCAGCTAAGCGGCCGC

Amino Acid Sequence

MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEV
VLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDRE
PLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGLEV
LFQGPGSMSEDSSALPWSINRDDYELQEVIGSGATAVVQAAYCAPKKEKVAIKRINLEKCQTSMDELLKEIQAMSQCHHPNIVSYYTSFVVKDELWLVMK
LLSGGSVLDIIKHIVAKGEHKSGVLDESTIATILREVLEGLEYLHKNGQIHRDVKAGNILLGEDGSVQIADFGVSAFLATGGDITRNKVRKTFVGTPCWM
APEVMEQVRGYDFKADIWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPSLETGVQDKEMLKKYGKSFRKMISLCLQKDPEKRPTAAELLRHKFFQK
AKNKEFLQEKTLQRAPTISERAKKVRRVPGSSGRLHKTEDGGWEWSDDEFDEESEEGKAAISQLRSPLVKESISNSELFPTTDPVGTLLQVPEQISAHLP
QPAGQIATQPTQVSLPPTAEPAKTAQALSSGSGSQETKIPISLVLRLRNSKKELNDIRFEFTPGRATAEGVSQELISAGLVDGRDLVIVAANLQKIVEEP
QSNRSVTFKLASGVEGSDIPDDGKLIGFAQLSIS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU39877
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: also has a R361L mutation
• Low Temp: No