cDNA Clone FIS1

• Synonyms: CGI-135, TTC11
• Plasmid: pBabe.hygro-mCherry-GFP FIS 101-152(end)
• Parent Plasmid: pBabe hygro mCherry GFP
• Expressed: mCherry-GFP-FIS 101-152(end)
• Genbank: NM_016068.2 GI:151108472
• Tag: GFP
• Tag: mCherry
• Species: Human

Sequence of Insert

ctgcagcctgaatatgggccaaacaggatatctgtggtaagcagttcctg
ccccggctcagggccaagaacagatggaacagctgaatatgggccaaaca
ggatatctgtggtaagcagttcctgccccggctcagggccaagaacagat
ggtccccagatgcggtccagccctcagcagtttctagagaaccatcagat
gtttccagggtgccccaaggacctgaaatgaccctgtgccttatttgaac
taaccaatcagttcgcttctcgcttctgttcgcgcgcttctgctccccga
gctcaataaaagagcccacaacccctcactcggggcgccagtcctccgat
tgactgagtcgcccgggtacccgtgtatccaataaaccctcttgcagttg
catccgacttgtggtctcgctgttccttgggagggtctcctctgagtgat
tgactacccgtcagcgggggtctttcatttgggggctcgtccgggatcgg
gagacccctgcccagggaccaccgacccaccaccgggaggtaagctggcc
agcaacttatctgtgtctgtccgattgtctagtgtctatgactgatttta
tgcgcctgcgtcggtactagttagctaactagctctgtatctggcggacc
cgtggtggaactgacgagttctgaacacccggccgcaaccctgggagacg
tcccagggactttgggggccgtttttgtggcccgacctgaggaagggagt
cgatgtggaatccgaccccgtcaggatatgtggttctggtaggagacgag
aacctaaaacagttcccgcctccgtctgaatttttgctttcggtttggaa
ccgaagccgcgcgtcttgtctgctgcagcatcgttctgtgttgtctctgt
ctgactgtgtttctgtatttgtctgaaaattagggccagactgttaccac
tcccttaagtttgaccttagatcactggaaagatgtcgagcggctcgctc
acaaccagtcggtagatgtcaagaagagacgttgggttaccttctgctct
gcagaatggccaacctttaacgtcggatggccgcgagacggcacctttaa
ccgagacctcatcacccaggttaagatcaaggtcttttcacctggcccgc
atggacacccagaccaggtcccctacatcgtgacctgggaagccttggct
tttgacccccctccctgggtcaagccctttgtacaccctaagcctccgcc
tcctcttcttccatccgcgccgtctctcccccttgaacctcctctttcga
ccccgcctcaatcctccctttatccagccctcactccttctctaggcgcc
ggccggatctatggtgagcaagggcgaggaggataacatggccatcatca
aggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccac
gagttcgagatcgagggcgagggcgagggccgcccctacgagggcaccca
gaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctggg
acatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcac
cccgccgacatccccgactacttgaagctgtccttccccgagggcttcaa
gtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgaccc
aggactcctccctgcaggacggcgagttcatctacaaggtgaagctgcgc
ggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatggg
ctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagg
gcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgct
gaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgc
ctacaacgtcaacatcaagttggacatcacctcccacaacgaggactaca
ccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggc
atggacgagctgtacaagggaggtggatctATGGTGAGCAAGGGCGAGGA
GCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAA
ACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTAC
GGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCC
CTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCC
GCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCC
GAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTA
CAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCA
TCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCAC
AAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAA
GCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGG
ACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGC
GACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGC
CCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGT
TCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGtcc
ggactcggatccgagccccagaacaaccaggccaaggaactggagcggct
cattgacaaggccatgaagaaagatggactcgtgggcatggccatcgtgg
gaggcatggccctgggtgtggcgggactggccggactcatcggacttgct
gtgtccaagtccaaatcctgagcggccgcccaagggcgaattcggccata
tgggggtcgaccctgtggaatgtgtgtcagttagggtgtggaaagtcccc
aggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcag
caaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaa
agcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcc
catcccgcccctaactccgcccagttccgcccattctccgccccatggct
gactaattttttttatttatgcagaggccgaggccgcctcggcctctgag
ctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaa
aaagctttaccatgaaaaagcctgaactcaccgcgacgtctgtcgagaag
tttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcgga
gggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatg
tcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtt
tatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacat
tggggagttcagcgagagcctgacctattgcatctcccgccgtgcacagg
gtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcag
ccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagac
gagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacat
ggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaa
actgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatga
gctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacg
cggatttcggctccaacaatgtcctgacggacaatggccgcataacagcg
gtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgc
caacatcttcttctggaggccgtggttggcttgtatggagcagcagacgc
gctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgg
gcgtatatgctccgcattggtcttgaccaactctatcagagcttggttga
cggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcg
tccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagc
gcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaa
ccgacgccccagcactcgtccgagggcaaaggaTAGAGTATATGCCGACC
GGGATCtatcgataaaataaaagattttatttagtctccagaaaaagggg
ggaatgaaagaccccacctgtaggtttggcaagctagcttaagtaacgcc
attttgcaaggcatggaaaaatacataactgagaatagagaagttcagat
caaggtcaggaacagatggaacagctgaatatgggccaaacaggatatct
gtggtaagcagttcctgccccggctcagggccaagaacagatggaacagc
tgaatatgggccaaacaggatatctgtggtaagcagttcctgccccggct
cagggccaagaacagatggtccccagatgcggtccagccctcagcagttt
ctagagaaccatcagatgtttccagggtgccccaaggacctgaaatgacc
ctgtgccttatttgaactaaccaatcagttcgcttctcgcttctgttcgc
gcgcttctgctccccgagctcaataaaagagcccacaacccctcactcgg
ggcgccagtcctccgattgactgagtcgcccgggtacccgtgtatccaat
aaaccctcttgcagttgcatccgacttgtggtctcgctgttccttgggag
ggtctcctctgagtgattgactacccgtcagcgggggtctttcatttccg
acttgtggtctcgctgccttgggagggtctcctctgagtgattgactacc
cgtcagcgggggtcttcacatgcagcatgtatcaaaattaatttggtttt
ttttcttaagtatttacattaaatggccatagttgcattaatgaatcggc
caacgcgcggggagaggcggtttgcgtattggcgctcttccgcttcctcg
ctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagc
tcactcaaaggcggtaatacggttatccacagaatcaggggataacgcag
gaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaag
gccgcgttgctggcgtttttccataggctccgcccccctgacgagcatca
caaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaa
gataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccg
accctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgt
ggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcg
ttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgc
tgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacga
cttatcgccactggcagcagccactggtaacaggattagcagagcgaggt
atgtaggcggtgctacagagttcttgaagtggtggcctaactacggctac
actagaaggacagtatttggtatctgcgctctgctgaagccagttacctt
cggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggta
gcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaagga
tctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaa
cgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatct
tcacctagatccttttaaattaaaaatgaagtttgcggccgcaaatcaat
ctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatca
gtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcc
tgactccccgtcgtgtagataactacgatacgggagggcttaccatctgg
ccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatt
tatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcct
gcaactttatccgcctccatccagtctattaattgttgccgggaagctag
agtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgcta
caggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctcc
ggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaa
agcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccg
cagtgttatcactcatggttatggcagcactgcataattctcttactgtc
atgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtc
attctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaa
cacgggataataccgcgccacatagcagaactttaaaagtgctcatcatt
ggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgag
atccagttcgatgtaacccactcgtgcacccaactgatcttcagcatctt
ttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgcc
gcaaaaaagggaataagggcgacacggaaatgttgaatactcatactctt
cctttttcaatattattgaagcatttatcagggttattgtctcatgagcg
gatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgc
acatttccccgaaaagtgccac

Amino Acid Sequence

MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKGGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSEPQNNQAKELERLIDKAMKKDGLVGMAIVGGMALGVAGLAGLIGLAVSKSKS*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pBabe.hygro-mCherry-GFP FIS 101-152(end)44.85 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU40799
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Full vector sequence in 'Sequence of Insert' field. (Exact sequence of vector backbone not guaranteed ).
• Low Temp: No