cDNA Clone Mitofilin

• Plasmid: pBABE Hygro Mitofilin HA
• Parent Plasmid: pBABE hygro
• Expressed: Mitofilin-HA (C-terminal)
• Genbank: NM_006839
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGCTGCGGGCCTGTCAGTTATCGGGTGTGACCGCCGCCGCCCA GAGTTGTCTCTGTGGGAAGTTTGTCCTCCGTCCATTGCGACCATGCCGCA GATACTCTACTTCAGGCAGCTCTGGGTTGACTACTGGCAAAATTGCTGGA GCTGGCCTTTTGTTTGTTGGTGGAGGTATTGGTGGCACTATCCTATATGC CAAATGGGATTCCCATTTCCGGGAAAGTGTAGAGAAAACCATACCTTACT CAGACAAACTCTTCGAGATGGTTCTTGGTCCTGCAGCTTATAATGTTCCA TTGCCAAAGAAATCGATTCAGTCGGGTCCACTAAAAATCTCTAGTGTATC AGAAGTAATGAAAGAATCTAAACAGCCTGCCTCACAACTCCAAAAACAAA AGGGAGATACTCCAGCTTCAGCAACAGCACCTACAGAAGCGGCTCAAATT ATTTCTGCAGCAGGTGATACCCTGTCGGTCCCAGCCCCTGCAGTTCAGCC TGAGGAATCTTTAAAAACTGATCACCCTGAAATTGGTGAAGGAAAACCCA CACCTGCACTTTCAGAAGAAGCATCCTCATCTTCTATAAGGGAGCGACCA CCTGAAGAAGTTGCAGCTCGCCTTGCACAACAGGAAAAACAAGAACAAGT TAAAATTGAGTCTCTAGCCAAGAGCTTAGAAGATGCTCTGAGGCAAACTG CAAGTGTCACTCTGCAGGCTATTGCAGCTCAGAATGCTGCGGTCCAGGCT GTCAATGCACACTCCAACATATTGAAAGCCGCCATGGACAATTCTGAGAT TGCAGGCGAGAAGAAATCTGCTCAGTGGCGCACAGTGGAGGGTGCATTGA AGGAACGCAGAAAGGCAGTAGATGAAGCTGCCGATGCCCTTCTCAAAGCC AAAGAAGAGTTAGAGAAGATGAAAAGTGTGATTGAAAATGCAAAGAAAAA AGAGGTTGCTGGGGCCAAGCCTCATATAACTGCTGCAGAGGGTAAACTTC ACAACATGATAGTTGATCTGGATAATGTGGTCAAAAAGGTCCAAGCAGCT CAGTCTGAGGCTAAGGTTGTATCTCAGTATCATGAGCTGGTGGTCCAAGC TCGGGATGACTTTAAACGAGAGCTGGACAGTATTACTCCAGAAGTCCTTC CTGGGTGGAAAGGAATGAGTGTTTCAGACTTAGCTGACAAGCTCTCTACT GATGATCTGAACTCCCTCATTGCTCATGCACATCGTCGTATTGATCAGCT GAACAGAGAGCTGGCAGAACAGAAGGCCACCGAAAAGCAGCACATCACGT TAGCCTTGGAGAAACAAAAGCTGGAAGAAAAGCGGGCATTTGACTCTGCA GTAGCAAAAGCATTAGAACATCACAGAAGTGAAATACAGGCTGAACAGGA CAGAAAGATAGAAGAAGTCAGAGATGCCATGGAAAATGAAATGAGAACCC AGCTTCGCCGACAGGCAGCTGCCCACACTGATCACTTGCGAGATGTCCTT AGGGTACAAGAACAGGAATTGAAGTCTGAATTTGAGCAGAACCTGTCTGA GAAACTCTCTGAACAAGAATTACAATTTCGTCGTCTCAGTCAAGAGCAAG TTGACAACTTTACTCTGGATATAAATACTGCCTATGCCAGACTCAGAGGA ATCGAACAGGCTGTTCAGAGCCATGCAGTTGCTGAAGAGGAAGCCAGAAA AGCCCACCAACTCTGGCTTTCAGTGGAGGCATTAAAGTACAGCATGAAGA CCTCATCTGCAGAAACACCTACTATCCCGCTGGGTAGTGCgGTTGAGGCC ATCAAAGCCAACTGTTCTGATAATGAATTCACCCAAGCTTTAACCGCAGC TATCCCTCCAGAGTCCCTGACCCGTGGGGTGTACAGTGAAGAGACCCTTA GAGCCCGTTTCTATGCTGTTCAAAAACTGGCCCGAAGGGTAGCAATGATT GATGAAACCAGAAATAGCTTGTACCAGTACTTCCTCTCCTACCTACAGTC CCTGCTCCTATTCCCACCTCAGCAACTGAAGCCGCCCCCAGAGCTCTGCC CTGAGGATATAAACACATTTAAATTACTGTCATATGCTTCCTATTGCATT GAGCATGGTGATCTGGAGCTAGCAGCAAAGTTTGTCAATCAGCTGAAGGG GGAATCCAGACGAGTGGCACAGGACTGGCTGAAGGAAGCCCGAATGACCC TAGAAACGAAACAGATAGTGGAAATCCTGACAGCATATGCCAGCGCCGTA GGAATAGGAACCACTCAGGTGCAGCCAGAGGCGGCCGCTTACCCATACGA TGTGCCAGATTACGCCTAAGGATCC

Amino Acid Sequence

MLRACQLSGVTAAAQSCLCGKFVLRPLRPCRRYSTSGSSGLTTGKIAGAG
LLFVGGGIGGTILYAKWDSHFRESVEKTIPYSDKLFEMVLGPAAYNVPLP
KKSIQSGPLKISSVSEVMKESKQPASQLQKQKGDTPASATAPTEAAQIIS
AAGDTLSVPAPAVQPEESLKTDHPEIGEGKPTPALSEEASSSSIRERPPE
EVAARLAQQEKQEQVKIESLAKSLEDALRQTASVTLQAIAAQNAAVQAVN
AHSNILKAAMDNSEIAGEKKSAQWRTVEGALKERRKAVDEAADALLKAKE
ELEKMKSVIENAKKKEVAGAKPHITAAEGKLHNMIVDLDNVVKKVQAAQS
EAKVVSQYHELVVQARDDFKRELDSITPEVLPGWKGMSVSDLADKLSTDD
LNSLIAHAHRRIDQLNRELAEQKATEKQHITLALEKQKLEEKRAFDSAVA
KALEHHRSEIQAEQDRKIEEVRDAMENEMRTQLRRQAAAHTDHLRDVLRV
QEQELKSEFEQNLSEKLSEQELQFRRLSQEQVDNFTLDINTAYARLRGIE
QAVQSHAVAEEEARKAHQLWLSVEALKYSMKTSSAETPTIPLGSAVEAIK
ANCSDNEFTQALTAAIPPESLTRGVYSEETLRARFYAVQKLARRVAMIDE
TRNSLYQYFLSYLQSLLLFPPQQLKPPPELCPEDINTFKLLSYASYCIEH
GDLELAAKFVNQLKGESRRVAQDWLKEARMTLETKQIVEILTAYASAVGI
GTTQVQPEAAAYPYDVPDYA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU43914
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: To be subcloned from DU38274 into pBABE.hygro vector