cDNA Clone NotIM

• Plasmid: pSCB BLA-6xHIS-NotIM EcoNI SalI
• Parent Plasmid: pSC-B
• Genbank: DQ242471.1
• Tag: 6His

Sequence of Insert

CCTGCATTAGGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATA
TTGAAAAAGGAAGAGTGGATCCATGCATCACCATCACCATCACAGCGGCC
CTCGACTGCACGTCGGTATCAGTGTGTGCGGATCGTTGACGGGGCTGTGG
ATAACTAGGTCGAGGAGAACACCTGTTCGTGTCGTGATACCTTATGATGC
CTGCGTGACGTCCTTGAGCAGCTATGACTCAGCAATCGATGACCCTACTG
TCGAGGTGCGGCAGGGGGACGCATACGATTTGGCCAGTGGTCTCGATCCG
CAGTCGATAGACCTCATCATCACGTCACCTCCTTACTGGGGGATGCGGAC
CTACGGCCATGACCACAGCGAAGATGTGCTTGACGAGTGGGTGGCCGAGG
GCAATCATGCGACGGACGTACCGCCCTACGAGTGGTATCGCGAACACGGT
GGTTTGCTCGGGATGGAACCGATTCCGGAGTGGTTCATTTCTCATTTGGT
GGAGATCTTCGAGCGCTTGAGGCCAGCCCTGAAGCTTGGTGGCAGCGTGT
GGGTCAACCTTGGCGATACGTACTTCGCTCGGTGGTCCAGCATACGATCC
GACGGCCGCCAGGGACTCGGGGACAACCCGCGTACCCGACGTAAAACCCC
GATGGGCGGATACCGCCAGGAGAAGCAGCTAATGCTCATTCCATCGCGGT
TCGCGATTGCCATGCAAGACAAGCGCTGGATTCTGCGGAACGACTTGATC
TGGCACAAGCCCAACGTTGCACCGCGGCCCGAGAAAGACCGCCTGCGCCT
GGCTCATGAGCACTTCTTTCACTTCGTGCTGAGGCCGAAGGAAGGAAGGG
CGAAGTACTACTACGACACCAGTGCGGTCGAAGAAGGTACGCGCGACGTT
GTGACCGTGAACGTTCGCTCGGGTTCGGATGGGCACTCAGCGACCTTCCC
CCCGGACTTGATCCGCCCGAGGATCGAAAGCTCGTCGCCCGTCGGTGGGC
TCGTCCTGGACCCGTTCGCTGGGACCGGGCGTGCACTCGGAGTCGCGGCT
GAACTTGGACGCAGCGCAATCGGATTCGAGCTGAGTGAGGAGTTCACGCA
AGCTGCCACCCGCAACGCGGAGGCCAGCGCAAACGCTTTGCGTCTCCTCT
AGgtcgac

Amino Acid Sequence

MHHHHHHSGPRLHVGISVCGSLTGLWITRSRRTPVRVVIPYDACVTSLSS
YDSAIDDPTVEVRQGDAYDLASGLDPQSIDLIITSPPYWGMRTYGHDHSE
DVLDEWVAEGNHATDVPPYEWYREHGGLLGMEPIPEWFISHLVEIFERLR
PALKLGGSVWVNLGDTYFARWSSIRSDGRQGLGDNPRTRRKTPMGGYRQE
KQLMLIPSRFAIAMQDKRWILRNDLIWHKPNVAPRPEKDRLRLAHEHFFH
FVLRPKEGRAKYYYDTSAVEEGTRDVVTVNVRSGSDGHSATFPPDLIRPR
IESSSPVGGLVLDPFAGTGRALGVAAELGRSAIGFELSEEFTQAATRNAE
ASANALRLL*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU44573
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Extended Bla promoter upstream of NotIM and added 6xHIS to check for effective expression of the methylase in bugs