cDNA Clone FAM76A

• Plasmid: pGEX6P Halo TEV FAM76A
• Parent Plasmid: pGEX6P1 Halo TEV
• Expressed: GST-C3-Halo.TEV(not blunt) FAM76A
• Genbank: Q8TAV0
• Tag: GST
• Tag: HALO
• Species: Human

Sequence of Insert

GGATCCATGGCGGCGCTCTACGCCTGCACCAAGTGCCACCAGCGCTTCCCCTTCGAGGCGCTGTCTCAGGGGCAGCAGCTGTGCAAGGAATGTCGGATTGCACACCCTGTTGTGAAGTGCACCTACTGCAGGACTGAGTACCAGCAGGAGAGTAAAACCAATACAATATGCAAGAAATGTGCTCAGAACGTGCAGTTGTATGGAACGCCCAAACCTTGTCAGTATTGCAACATAATTGCAGCATTTATTGGGAATAAATGCCAGCGCTGCACAAATTCAGAAAAGAAGTATGGACCACCCTATTCTTGTGAACAGTGCAAGCAGCAGTGTGCATTTGACAGGAAAGATGATAGAAAGAAGGTAGATGGGAAATTGCTGTGCTGGCTGTGCACACTTTCATACAAACGGGTCCTTCAGAAGACCAAAGAGCAGAGGAAACACCTGAGTAGCTCTTCTCGTGCTGGCCACCAGGAGAAGGAGCAGTATAGTCGCCTGAGTGGTGGTGGCCATTATAACAGCCAGAAAACACTTTCTACATCTTCAATTCAAAATGAAATCCCAAAGAAAAAGTCCAAGTTTGAGTCAATCACAACTAATGGAGACAGCTTCTCCCCAGACCTGGCTCTGGACTCACCAGGCACTGACCACTTTGTCATCATTGCCCAACTGAAGGAAGAAGTGGCTACCCTGAAGAAGATGTTGCATCAAAAGGATCAAATGATTTTAGAGAAAGAGAAGAAGATTACAGAGTTGAAGGCTGATTTTCAGTACCAGGAATCGCAGATGAGAGCCAAAATGAACCAGATGGAGAAAACCCACAAAGAAGTCACAGAACAACTGCAGGCCAAAAACCGAGAGCTCCTGAAGCAGGCAGCTGCTTTGTCCAAGAGCAAGAAGTCAGAGAAGTCAGGAGCTATAACCTCTCCATGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGENLYFQGGSMAALYACTKCHQR
FPFEALSQGQQLCKECRIAHPVVKCTYCRTEYQQESKTNTICKKCAQNVQ
LYGTPKPCQYCNIIAAFIGNKCQRCTNSEKKYGPPYSCEQCKQQCAFDRK
DDRKKVDGKLLCWLCTLSYKRVLQKTKEQRKHLSSSSRAGHQEKEQYSRL
SGGGHYNSQKTLSTSSIQNEIPKKKSKFESITTNGDSFSPDLALDSPGTD
HFVIIAQLKEEVATLKKMLHQKDQMILEKEKKITELKADFQYQESQMRAK
MNQMEKTHKEVTEQLQAKNRELLKQAAALSKSKKSEKSGAITSP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU45562
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All FAM76a plasmids MUST be grown at 30 degrees C or less to prevent recombination.; ; parental 24005