cDNA Clone MAP4K1 mouse

• Plasmid: pFBDual6P-2 HPK1 (mouse)
• Parent Plasmid: pFastbacDual 6P2
• Expressed: GST-MAP4K1 mouse
• Genbank: NP_032305
• Tag: GST
• Species: Mouse

Sequence of Insert

GCGGCCGCGATGGCCCTTGTGGACCCCGACATTTTTAATAAGGACCCTCG
AGAACACTATGATCTACTGCAACGACTGGGTGGTGGAACTTATGGAGAAG
TATTCAAGGCTCGCGACAAGGTGTCTAAGGACCTGGTGGCTTTGAAGATG
GTGAAGATGGAGCCTGATGATGACGTCGCCACCCTTCAGAAGGAGATTCT
CATGCTGAAAACTTGCCGGCACGCCAACATCGTGGCTTACCATGGCAGTT
ACCTCTGGCTGCAGAAGCTGTGGATCTGCATGGAATTCTGTGGAGCTGGT
TCTCTCCAGGACATCTACCAAGTGACTGGCTCTCTGTCCGAGCTCCAGAT
CAGCTATGTGTGCCGGGAAGTGCTTCAGGGACTGGCCTACCTGCATTCAG
AGAAGAAGATACATCGAGACATCAAGGGAGCCAACATCCTCATCAACGAC
TGCGGGGAGGTTAAGCTGGCTGACTTTGGGATCTCAGCCCAGATTGGGGC
GACACTGGCCCGGCGCCTTTCTTTCATTGGGACACCGTACTGGATGGCTC
CAGAGGTGGCAGCGGTGGCCCTGAAGGGTGGATACAATGAGCTGTGCGAC
ATCTGGTCTCTTGGCATCACAGCCATCGAGCTGGCTGAGCTTCAGCCCCC
TCTCTTTGATGTGCACCCTCTCAGGGTTCTCTTCCTCATGACCAAGAGCG
GGTACCAGCCTCCTCGGCTGAAGGAAAAGAGCAGATGGTCATCTTCCTTC
CACAACTTTGTCAAAGTCACCCTCACTAAGAACTCCAAGAAACGCCCCAG
TGCCACCAAGATGCTCAGTCATCAGCTGGTGTCCCAGCCAGGGCTGAACA
GAGGCCTGATCTTGGATCTTCTTGACAAAATGAAGAATCCTGGGAAGGGG
CTGCCTGTTGATATTGAAGATGAGGAGCCTGAGCCACCCCCTGCCATCCC
TCGGCGGATCAGATCTACCTACCGGGCCAGCTCACTAGGGATCCCAGATG
CAGACTGCTGCCGGAGGCAAATGGAGTTCCAGAGGCCCAGATGCGTGGAC
TGCAGACCTCAGGCTGAAACTGTTCGCTTATATCCCCCTGCACACTTCGG
GAGCACCAGTCCCAGGAGTCAGCTGTCAGATTCTGATGACGACTATGATG
ATGTGGACATCCCTGCCCCTTCAGAGAACATACCTCCTCCACTGCCCCCC
AAGCCCAAGTTTCGGTCTCCATCAGATGATGGTTCTGGAGGAATTAGGGA
CGATGGGCAATTGAGCCCAGGGGTTCTGGTTCGATGTGCAAGTGGGCCCC
CTCCACGTACTCCCCGACCTGGACCTCCCCCAGCGACCTGCAGCCCTCAC
CTCACAGCTCGCTCAGATCCCTCATTGTGGAACCCAGCTGCTCCGGAGCC
AGGCCAGCCCCCACTCGTACCCCCAAGGAAGGAAAAGATGAGGGGAAAGA
TGGAGAACGAGAAGCGCAGAGAGAAATATCCCCTTCTTGTGAAATTATTC
AATGGCTGTCCCCTTCAGATCCACAGCACAGCTGCCTGGACACATCCCTC
TACAAAAGATCAGAACCTGCTCCTGGGGGCAGAGGAAGGCATTTTCATAC
TGAACCGGAATGATCAGGAGGCCACGCTGGAAATGATCTTCCCTGGCCGC
ACAACCTGGCTCTATTGCATCAACAACCTCCTCATGTCTCTCTCAGGGAA
GACACCCTACCTGTATTCTCATAGCATCCTGGGCCTGCTTGAAAGGAAAG
ACGGCAGAACGGGAAGCCCCATTGCTCACATTAGCCCTCACCGGTTACTG
GCAAGAAAGAACATGGTCTCCAGTAAGATCCAGGACACCAAAGGCTGCCG
GGCTTGCTGTGTGGCTGAGAGTGCTAGCTCTGGGGGCCCATTCTTATGTG
GGGCATTGGAGACGTCTGTGGTTTTGCTCCAGTGGTACCAACCAATGAAC
AAATTCTTGCTTGTCCGGCAGGTGCTGTTCCCGCTGCCCACGCCGCTGCC
GGTGTTCACGCTGCTGACAACGCCGGGCTCCGAGCTGCCAGCTGTGTGCA
TCGGCGTGAGCCCGGGGCAGGCGGCAAAGTCGGTGCTCTTCCACACCGTG
CGCTTCGGTGCGCTGTCCTGCTGGCTGGACGACTCCAGCACGGAGCACAA
GGGACCAGTGCAGGTGATCCAAGTAAAGGAAGACATGGTGATGGTGTTGA
TGGATGGCTCTCTAAAGCTAGTGACCCCAGAGGGGGCACCAGCTCCTGGG
CTCCGCACCCCTGAGATCCCCATGACAGAAGCGGTCGAGGCTGTGGCTAT
GGTTGAAGATCGACTCGAGGCCTTCTGGAAGCATGGAGTTCAGGTGTGGG
CTCCAGGCTTGAAACAGCCGCTGCAGGAGCTGAGAGACCCCACCCTCACC
TTCCGTCTGCTCTGCTCCCCCAGGCCGGTAGTGGTGGAGACAAGGCCCAC
GGATGACCCTACGGCCCCCAGCAACCTCTACATCCAGGAATGAGCGGCCG
C

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMALVDPDI
FNKDPREHYDLLQRLGGGTYGEVFKARDKVSKDLVALKMVKMEPDDDVAT
LQKEILMLKTCRHANIVAYHGSYLWLQKLWICMEFCGAGSLQDIYQVTGS
LSELQISYVCREVLQGLAYLHSEKKIHRDIKGANILINDCGEVKLADFGI
SAQIGATLARRLSFIGTPYWMAPEVAAVALKGGYNELCDIWSLGITAIEL
AELQPPLFDVHPLRVLFLMTKSGYQPPRLKEKSRWSSSFHNFVKVTLTKN
SKKRPSATKMLSHQLVSQPGLNRGLILDLLDKMKNPGKGLPVDIEDEEPE
PPPAIPRRIRSTYRASSLGIPDADCCRRQMEFQRPRCVDCRPQAETVRLY
PPAHFGSTSPRSQLSDSDDDYDDVDIPAPSENIPPPLPPKPKFRSPSDDG
SGGIRDDGQLSPGVLVRCASGPPPRTPRPGPPPATCSPHLTARSDPSLWN
PAAPEPGQPPLVPPRKEKMRGKMENEKRREKYPLLVKLFNGCPLQIHSTA
AWTHPSTKDQNLLLGAEEGIFILNRNDQEATLEMIFPGRTTWLYCINNLL
MSLSGKTPYLYSHSILGLLERKDGRTGSPIAHISPHRLLARKNMVSSKIQ
DTKGCRACCVAESASSGGPFLCGALETSVVLLQWYQPMNKFLLVRQVLFP
LPTPLPVFTLLTTPGSELPAVCIGVSPGQAAKSVLFHTVRFGALSCWLDD
SSTEHKGPVQVIQVKEDMVMVLMDGSLKLVTPEGAPAPGLRTPEIPMTEA
VEAVAMVEDRLEAFWKHGVQVWAPGLKQPLQELRDPTLTFRLLCSPRPVV
VETRPTDDPTAPSNLYIQE*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU43899
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Make the same as the human version