cDNA Clone FANCI (Xenopus)

• Plasmid: pFastBac-FLAG-FANCI (Xenopus) D338G, C781Y, A1082V, V1244A
• Parent Plasmid: pFastbac1
• Expressed: Flag-FANCI D338G, C781Y, A1082V, V1244A
• Genbank: NM_001171211.1.
• Tag: FLAG
• Species: Xenopus laevis

Amino Acid Sequence

MDYKDDDDKDQKILSLAAEEQNDGLQSYLQNLKETELSEIITKHAVKGKD
CGALLRGVFKGSPCSHDVAVRRRLAVYRHCIQLVESGDLQREVASEIMGL
LMLEVHHFPGASLIELANLFADAVKAGTLSNGKSLDLFPTVLTALSSKES
LAYGKGELTGEEFKKQLINSLCSSRWDPQSVIFLTSMFRDVSLSAEELQF
VVEKVLRMLSKLDLQEMPPLVYQLLLLSAKGSKKNIVEGIITIFNDLDQK
QLVQQENSESLDLEDATIPQDQLHHVEGTIILHIVFAIKLDQELGRELLK
YLKAGQQGDSSKILCPFSAALLLSVSRIHRFQEQVFDFLKSTILRGYKDL
QFHQSSKFLQDLVPQPVCVSSILLETVKNSVYGWDHVTQGLVELGFILMD
SFGPKAAFGNKVVELISVHSRTPSQQACQLGSRILLETFKVHEPIRSEIL
EQVLNRVITKAATPVTHFIDLLSDIVVSAPLILQNSSSKVTEAFDHLSFL
PLTTVQGLLKAVQPLLKISMSMRDSLILVLRKAMFSSQIDARKSAVAGFL
LLLRNFKVLGSLSSSQCSQAIGASQIQVDVHMRYNAAANEAFCLEILGSL
RRCLSQQADVRLMLYEGFYDVLRRNSQLASSVMQTLLSQLKRYYEPEPDL
LPPLKLEGCITAQGDHIFLQEPLAHLLCCIHHCLQWYKSSLQQHRNPDED
DDDDDQTGCQQDLNDIMESITRRMIKCDLEDFELDKSADFSLASGVGVKN
NIYAVLVMGICEVLIEYNFIIANFSKSKFEDILGLFKCYSKLSDILKEKA
AKGRQPGNNKTARSLISMTFVSTLLTALFRDSTRSHEESLSILRANVDFM
RYSVCVALQKIQQLEETGVTDGPDGQNSEKMFRSLCEITRVLMWRYTSIP
AAAENPGKKDKGKTISLLCLEGLLRVFNTVQQRYPSKIPQFLTALDALGD
EDEEGSREINVTEKAAFQIKQFQRSLINQLSGGEDDFNSKEALLLVSILS
TLSRLLAPSSQQFVQMLSWTVKICKETNIEDVQFCKGIMNLLFSLHVQFK
SPVSVLRELCQDIHGHLGDIDQDIEVEKQSHFATVSLKTVAPTVTLLVLG
QAAKVLEEVDWLIIKLKGLLGSEKLSTEDLTQTSNARVPIEKAIILQLGT
LLTACHELVQTALPAGSCTDTLLKELAKMYTILTSVVKYYLQICSSHGGQ
ISARLEKLVKLSGSHLTPQCYAFITYVQNIHSESLSLAAEKKKKKKEEAL
TATSAKILRDTKPIPNLIFAIEQYEKFLIHLSKKSKVNLMQYMKLSTSRD
FRINAATLDAALQEKGSEDEENEPDNEQAVTEEESQEPKKKRRRK*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU24022
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Clone has D338G, C781Y, A1082V, V1244A substitutions compared to NCBI ref. Also harbours silent mutation c3753t (R1251); ; All FANCI plasmids MUST be grown at 30 degrees C or less to prevent recombination.