cDNA Clone LRRK2

• Plasmid: pCMVHA-3
• Parent Plasmid: pCMV5 HA3
• Expressed: pCMVHA-3 6xNictide
• Tag: HA

Sequence of Insert

GGATCCCCGGAATTCCCGGGTCGAGGTCCTCTTCCAGGGCCCCCTCGGCG
CCCGCCTCGGCTGGTGGCGCTTCTACACCCTCCGCCGCGCCCGCCAGGGC
AACACCAAGCAGCGCCTCGGCGCCCGCCTCGGCTGGTGGCGCTTCTACAC
CCTCCGCCGCGCCCGCCAGGGCAACACCAAGCAGCGCCTCGGCGCCCGCC
TCGGCTGGTGGCGCTTCTACACCCTCCGCCGCGCCCGCCAGGGCAACACC
AAGCAGCGCCTCGGCGCCCGCCTCGGCTGGTGGCGCTTCTACACCCTCCG
CCGCGCCCGCCAGGGCAACACCAAGCAGCGCCTCGGCGCCCGCCTCGGCT
GGTGGCGCTTCTACACCCTCCGCCGCGCCCGCCAGGGCAACACCAAGCAG
CGCCTCGGCGCCCGCCTCGGCTGGTGGCGCTTCTACACCCTCCGCCGCGC
CCGCCAGGGCAACACCAAGCAGCGCTAGGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGDPRNSRVEVLFQGPLGARLGWWRFYTLRRARQGNTKQRL
GARLGWWRFYTLRRARQGNTKQRLGARLGWWRFYTLRRARQGNTKQRLGA
RLGWWRFYTLRRARQGNTKQRLGARLGWWRFYTLRRARQGNTKQRLGARL
GWWRFYTLRRARQGNTKQR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU43824
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All LRRK2 plasmids MUST be grown at 30°C or less to prevent recombination Contains SNP S1647T