cDNA Clone FANCI

• Plasmid: pCMV-Myc-1-FANCI S556D/S559D/S565D/S596D R1285Q
• Parent Plasmid: pCMV5 Myc1
• Expressed: Myc-FANCI S556D/S559D/S565D/S596D R1285Q
• Genbank: NM_001113378.1.
• Tag: Myc
• Species: Human

Sequence of Insert

GGATCCatggaccagaagattttatctctagcagcagaaaaaacagcaga
caaactgcaagaatttcttcaaaccctgagagaaggtgatttgactaatc
tccttcagaatcaagcagtgaaaggaaaagttgctggagcactcctgaga
gccatcttcaaaggttccccctgctctgaggaagctggaacacttaggag
acgtaagatatacacttgttgtatccagttggtggaatcgggggatttgc
agaaagaaatagcgtctgagatcataggattactgatgctggaggctcac
cattttccaggaccattattggttgaattagccaatgagtttattagtgc
tgtcagagaaggcagcctagtgaatggaaaatctttggagttactaccta
tcattctcactgccctggctacgaaaaaggaaaatctggcttatggaaaa
ggtgtactgagtggggaagaatgtaagaaacagttgattaacaccctgtg
ttctggcaggtgggatcagcaatatgtaatccaactcacctccatgttca
aggatgtccctctgactgcagaagaggtggaatttgtggtggaaaaagca
ttgagcatgttctccaagatgaatcttcaagaaataccacctttggtcta
tcagcttctggttctctcctccaagggaagcagaaagagtgttttggaag
gaatcatagccttcttcagtgcactagataagcagcacaatgaggaacag
agtggtgacgagctattggatgttgtcactgtgccatcaggtgaacttcg
tcatgtggaaggcaccattattctacacattgtgtttgccatcaaattgg
actatgaactaggcagagaactcgtgaaacacttaaaggtaggacagcaa
ggagattccaataataacttaagtcccttcagcattgctcttcttctgtc
tgtaacaagaatacaaagatttcaggaccaggtgcttgatcttttaaaga
cttcggttgtaaagagctttaaggatcttcaactcctccaaggctcaaaa
tttcttcagaatctagttcctcatagatcttatgtttcaaccatgatctt
ggaagtagtgaagaatagcgttcatagctgggaccatgttactcagggcc
tcgtagaacttggtttcattttgatggattcatatgggccaaagaaggtt
cttgatggaaaaactattgaaaccagcccaagtctttctagaatgccaaa
ccagcatgcatgtaagctcggagctaatatcctgttggaaacttttaaga
tccatgagatgatcagacaagaaattttggagcaggtcctcaacagggtt
gttaccagagcatcttctcccatcagtcatttcttagacctgctttcaaa
tatcgtcatgtatgcacccttagttcttcaaagttgttcttctaaagtca
cagaagcttttgactatttgtcctttctgccccttcagactgtacaaagg
ctgcttaaggcagtgcagccccttctcaaagtcagcatgtcaatgagaga
ctgcttgatacttgtccttcggaaagctatgtttgccaaccagcttgatg
cccgaaaatctgcagttgctgggtttttgctgctcctgaagaactttaaa
gttttaggcagcctgtcatccGAtcagtgcGAtcagtctctcagtgtcGA
tcaggttcatgtggatgttcacagccattacaattctgtcgccaatgaaa
ctttttgccttgagatcatggatagtttgaggagatgcttaGAccagcaa
gctgatgttcgactcatgctttatgaggggttttatgatgttcttcgaag
gaactctcagctggctaattcagtcatgcaaactctgctctctcagttaa
aacagttctatgagccaaaacctgatctgctgcctcctctgaaattagaa
gcttgtattctgacccaaggagataagatctctctacaagaaccactgga
ttatctgctgtgttgtattcagcattgtttggcctggtataagaatacag
tcatacccttacagcagggagaggaggaagaggaggaggaagaggcattc
tacgaagacctagatgatatattggagtccattactaatagaatgattaa
gagtgagctggaagactttgaactggataaatcagcagatttttctcaga
gcaccagtattggcataaaaaataatatctgtgcttttcttgtgatggga
gtttgtgaggttttaatagaatacaatttctccataagtagtttcagtaa
gaataggtttgaggacattctgagcttatttatgtgttacaaaaaactct
ctgacattcttaatgaaaaagcgggtaaagccaaaactaaaatggccaac
aagacaagtgatagtcttttgtccatgaaatttgtgtccagtcttctcac
tgctcttttcagggatagtatccaaagccaccaagaaagcctttctgttc
tcaggtccagcaatgagtttatgcgctatgcagtgaatgtagctctgcag
aaagtacagcagctaaaggaaacagggcatgtgagtggccctgatggcca
aaacccagaaaagatctttcagaacctctgtgacataactcgagtcttgc
tatggagatacacttcaattcctacttcagtggaagagtcgggaaagaaa
gagaaaggaaagagcatctcactgctgtgcttggagggtttacagaaaat
attcagtgctgtgcaacagttctatcagcccaagattcagcagtttctca
gagctctggatgtcacagataaggaaggagaagagagagaagatgcagat
gtcagtgtcactcagagaacagcattccagatccggcaatttcagaggtc
cttgttgaatttacttagcagtcaagaggaagattttaatagcaaagaag
ccctcctgctagtcacggttcttaccagtttgtccaagttactggagccc
tcctctcctcagtttgtgcagatgttatcctggacatcaaagatttgcaa
ggaaaacagccgggaggatgccttgttttgcaagagcttgatgaacttgc
tcttcagcctgcatgtttcgtataagagtcctgtcattctgctgcgtgac
ttgtcccaggatatccacgggcatctgggagatatagaccaggatgtaga
ggtggagaaaacaaaccactttgcaatagtgaatttgagaacggctgccc
ccactgtctgtttacttgttctgagtcaggccgagaaggttctagaagaa
gtggactggctaatcaccaagcttaagggacaagtgagccaagaaacctt
atcagaagaggcctcttctcaggcaaccctaccaaatcagcctgttgaga
aagctatcatcatgcaactgggaactctgcttacatttttccacgagctg
gtgcagacagctctgccatcaggcagctgtgtggacaccttgttaaagga
cttgtgcaaaatgtacaccacacttacagcccttgtcagatattatctcc
aggtgtgtcagagctccggaggaattccaaaaaatatggaaaagctggtg
aagctgtctggttctcatctgacccccctgtgttattctttcatttctta
cgtacagaataagagtaagagcctgaactatacgggagagaaaaaggaga
aacctgctgccgttgccacagccatggccagagttcttcgggaaaccaag
ccaatccctaacctcatctttgccatagaacagtatgaaaaatttctcat
ccacctttctaagaagtccaaggtgaacctgatgcagcacatgaagctca
gcacctcacaagacttcaagatcaaaggaaacatcctagacatggttctt
cgagaggatggtgaagatgaaaatgaagagggcactgcatcagagcatgg
gggacagaacaaagaaccagccaagaagaaaaggaaaaaataaGCGGCCG
C

Amino Acid Sequence

MMEQKLISEEDLGSMDQKILSLAAEKTADKLQEFLQTLREGDLTNLLQNQ
AVKGKVAGALLRAIFKGSPCSEEAGTLRRRKIYTCCIQLVESGDLQKEIA
SEIIGLLMLEAHHFPGPLLVELANEFISAVREGSLVNGKSLELLPIILTA
LATKKENLAYGKGVLSGEECKKQLINTLCSGRWDQQYVIQLTSMFKDVPL
TAEEVEFVVEKALSMFSKMNLQEIPPLVYQLLVLSSKGSRKSVLEGIIAF
FSALDKQHNEEQSGDELLDVVTVPSGELRHVEGTIILHIVFAIKLDYELG
RELVKHLKVGQQGDSNNNLSPFSIALLLSVTRIQRFQDQVLDLLKTSVVK
SFKDLQLLQGSKFLQNLVPHRSYVSTMILEVVKNSVHSWDHVTQGLVELG
FILMDSYGPKKVLDGKTIETSPSLSRMPNQHACKLGANILLETFKIHEMI
RQEILEQVLNRVVTRASSPISHFLDLLSNIVMYAPLVLQSCSSKVTEAFD
YLSFLPLQTVQRLLKAVQPLLKVSMSMRDCLILVLRKAMFANQLDARKSA
VAGFLLLLKNFKVLGSLSSDQCDQSLSVDQVHVDVHSHYNSVANETFCLE
IMDSLRRCLDQQADVRLMLYEGFYDVLRRNSQLANSVMQTLLSQLKQFYE
PKPDLLPPLKLEACILTQGDKISLQEPLDYLLCCIQHCLAWYKNTVIPLQ
QGEEEEEEEEAFYEDLDDILESITNRMIKSELEDFELDKSADFSQSTSIG
IKNNICAFLVMGVCEVLIEYNFSISSFSKNRFEDILSLFMCYKKLSDILN
EKAGKAKTKMANKTSDSLLSMKFVSSLLTALFRDSIQSHQESLSVLRSSN
EFMRYAVNVALQKVQQLKETGHVSGPDGQNPEKIFQNLCDITRVLLWRYT
SIPTSVEESGKKEKGKSISLLCLEGLQKIFSAVQQFYQPKIQQFLRALDV
TDKEGEEREDADVSVTQRTAFQIRQFQRSLLNLLSSQEEDFNSKEALLLV
TVLTSLSKLLEPSSPQFVQMLSWTSKICKENSREDALFCKSLMNLLFSLH
VSYKSPVILLRDLSQDIHGHLGDIDQDVEVEKTNHFAIVNLRTAAPTVCL
LVLSQAEKVLEEVDWLITKLKGQVSQETLSEEASSQATLPNQPVEKAIIM
QLGTLLTFFHELVQTALPSGSCVDTLLKDLCKMYTTLTALVRYYLQVCQS
SGGIPKNMEKLVKLSGSHLTPLCYSFISYVQNKSKSLNYTGEKKEKPAAV
ATAMARVLRETKPIPNLIFAIEQYEKFLIHLSKKSKVNLMQHMKLSTSQD
FKIKGNILDMVLREDGEDENEEGTASEHGGQNKEPAKKKRKK*AA

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24039
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All FANCI plasmids MUST be grown at 30 degrees C or less to prevent recombination.