cDNA Clone FANCI

• Plasmid: pCMV-Myc-1-FANCI R1285Q
• Parent Plasmid: pCMV5 Myc1
• Expressed: Myc-FANCI R1285Q
• Genbank: NM_001113378.1.
• Tag: Myc
• Species: Human

Sequence of Insert

GGATCCATGGACCAGAAGATTTTATCTCTAGCAGCAGAAAAAACAGCAGA
CAAACTGCAAGAATTTCTTCAAACCCTGAGAGAAGGTGATTTGACTAATC
TCCTTCAGAATCAAGCAGTGAAAGGAAAAGTTGCTGGAGCACTCCTGAGA
GCCATCTTCAAAGGTTCCCCCTGCTCTGAGGAAGCTGGAACACTTAGGAG
ACGTAAGATATACACTTGTTGTATCCAGTTGGTGGAATCGGGGGATTTGC
AGAAAGAAATAGCGTCTGAGATCATAGGATTACTGATGCTGGAGGCTCAC
CATTTTCCAGGACCATTATTGGTTGAATTAGCCAATGAGTTTATTAGTGC
TGTCAGAGAAGGCAGCCTAGTGAATGGAAAATCTTTGGAGTTACTACCTA
TCATTCTCACTGCCCTGGCTACGAAAAAGGAAAATCTGGCTTATGGAAAA
GGTGTACTGAGTGGGGAAGAATGTAAGAAACAGTTGATTAACACCCTGTG
TTCTGGCAGGTGGGATCAGCAATATGTAATCCAACTCACCTCCATGTTCA
AGGATGTCCCTCTGACTGCAGAAGAGGTGGAATTTGTGGTGGAAAAAGCA
TTGAGCATGTTCTCCAAGATGAATCTTCAAGAAATACCACCTTTGGTCTA
TCAGCTTCTGGTTCTCTCCTCCAAGGGAAGCAGAAAGAGTGTTTTGGAAG
GAATCATAGCCTTCTTCAGTGCACTAGATAAGCAGCACAATGAGGAACAG
AGTGGTGACGAGCTATTGGATGTTGTCACTGTGCCATCAGGTGAACTTCG
TCATGTGGAAGGCACCATTATTCTACACATTGTGTTTGCCATCAAATTGG
ACTATGAACTAGGCAGAGAACTCGTGAAACACTTAAAGGTAGGACAGCAA
GGAGATTCCAATAATAACTTAAGTCCCTTCAGCATTGCTCTTCTTCTGTC
TGTAACAAGAATACAAAGATTTCAGGACCAGGTGCTTGATCTTTTAAAGA
CTTCGGTTGTAAAGAGCTTTAAGGATCTTCAACTCCTCCAAGGCTCAAAA
TTTCTTCAGAATCTAGTTCCTCATAGATCTTATGTTTCAACCATGATCTT
GGAAGTAGTGAAGAATAGCGTTCATAGCTGGGACCATGTTACTCAGGGCC
TCGTAGAACTTGGTTTCATTTTGATGGATTCATATGGGCCAAAGAAGGTT
CTTGATGGAAAAACTATTGAAACCAGCCCAAGTCTTTCTAGAATGCCAAA
CCAGCATGCATGTAAGCTCGGAGCTAATATCCTGTTGGAAACTTTTAAGA
TCCATGAGATGATCAGACAAGAAATTTTGGAGCAGGTCCTCAACAGGGTT
GTTACCAGAGCATCTTCTCCCATCAGTCATTTCTTAGACCTGCTTTCAAA
TATCGTCATGTATGCACCCTTAGTTCTTCAAAGTTGTTCTTCTAAAGTCA
CAGAAGCTTTTGACTATTTGTCCTTTCTGCCCCTTCAGACTGTACAAAGG
CTGCTTAAGGCAGTGCAGCCCCTTCTCAAAGTCAGCATGTCAATGAGAGA
CTGCTTGATACTTGTCCTTCGGAAAGCTATGTTTGCCAACCAGCTTGATG
CCCGAAAATCTGCAGTTGCTGGGTTTTTGCTGCTCCTGAAGAACTTTAAA
GTTTTAGGCAGCCTGTCATCCTCTCAGTGCAGTCAGTCTCTCAGTGTCAG
TCAGGTTCATGTGGATGTTCACAGCCATTACAATTCTGTCGCCAATGAAA
CTTTTTGCCTTGAGATCATGGATAGTTTGAGGAGATGCTTAAGCCAGCAA
GCTGATGTTCGACTCATGCTTTATGAGGGGTTTTATGATGTTCTTCGAAG
GAACTCTCAGCTGGCTAATTCAGTCATGCAAACTCTGCTCTCACAGTTAA
AACAGTTCTATGAGCCAAAACCTGATCTGCTGCCTCCTCTGAAATTAGAA
GCTTGTATTCTGACCCAAGGAGATAAGATCTCTCTACAAGAACCACTGGA
TTATCTGCTGTGTTGTATTCAGCATTGTTTGGCCTGGTATAAGAATACAG
TCATACCCTTACAGCAGGGAGAGGAGGAAGAGGAGGAGGAAGAGGCATTC
TACGAAGACCTAGATGATATATTGGAGTCCATTACTAATAGAATGATTAA
GAGTGAGCTGGAAGACTTTGAACTGGATAAATCAGCAGATTTTTCTCAGA
GCACCAGTATTGGCATAAAAAATAATATCTGTGCTTTTCTTGTGATGGGA
GTTTGTGAGGTTTTAATAGAATACAATTTCTCCATAAGTAGTTTCAGTAA
GAATAGGTTTGAGGACATTCTGAGCTTATTTATGTGTTACAAAAAACTCT
CTGACATTCTTAATGAAAAAGCGGGTAAAGCCAAAACTAAAATGGCCAAC
AAGACAAGTGATAGTCTTTTGTCCATGAAATTTGTGTCCAGTCTTCTCAC
TGCTCTTTTCAGGGATAGTATCCAAAGCCACCAAGAAAGCCTTTCTGTTC
TCAGGTCCAGCAATGAGTTTATGCGCTATGCAGTGAATGTAGCTCTGCAG
AAAGTACAGCAGCTAAAGGAAACAGGGCATGTGAGTGGCCCTGATGGCCA
AAACCCAGAAAAGATCTTTCAGAACCTCTGTGACATAACTCGAGTCTTGC
TATGGAGATACACTTCAATTCCTACTTCAGTGGAAGAGTCGGGAAAGAAA
GAGAAAGGAAAGAGCATCTCACTGCTGTGCTTGGAGGGTTTACAGAAAAT
ATTCAGTGCTGTGCAACAGTTCTATCAGCCCAAGATTCAGCAGTTTCTCA
GAGCTCTGGATGTCACAGATAAGGAAGGAGAAGAGAGAGAAGATGCAGAT
GTCAGTGTCACTCAGAGAACAGCATTCCAGATCCGGCAATTTCAGAGGTC
CTTGTTGAATTTACTTAGCAGTCAAGAGGAAGATTTTAATAGCAAAGAAG
CCCTCCTGCTAGTCACGGTTCTTACCAGTTTGTCCAAGTTACTGGAGCCC
TCCTCTCCTCAGTTTGTGCAGATGTTATCCTGGACATCAAAGATTTGCAA
GGAAAACAGCCGGGAGGATGCCTTGTTTTGCAAGAGCTTGATGAACTTGC
TCTTCAGCCTGCATGTTTCGTATAAGAGTCCTGTCATTCTGCTGCGTGAC
TTGTCCCAGGATATCCACGGGCATCTGGGAGATATAGACCAGGATGTAGA
GGTGGAGAAAACAAACCACTTTGCAATAGTGAATTTGAGAACGGCTGCCC
CCACTGTCTGTTTACTTGTTCTGAGTCAGGCCGAGAAGGTTCTAGAAGAA
GTGGACTGGCTAATCACCAAGCTTAAGGGACAAGTGAGCCAAGAAACCTT
ATCAGAAGAGGCCTCTTCTCAGGCAACCCTACCAAATCAGCCTGTTGAGA
AAGCTATCATCATGCAACTGGGAACTCTGCTTACATTTTTCCACGAGCTG
GTGCAGACAGCTCTGCCATCAGGCAGCTGTGTGGACACCTTGTTAAAGGA
CTTGTGCAAAATGTACACCACACTTACAGCCCTTGTCAGATATTATCTCC
AGGTGTGTCAGAGCTCCGGAGGAATTCCAAAAAATATGGAAAAGCTGGTG
AAGCTGTCTGGTTCTCATCTGACCCCCCTGTGTTATTCTTTCATTTCTTA
CGTACAGAATAAGAGTAAGAGCCTGAACTATACGGGAGAGAAAAAGGAGA
AACCTGCTGCCGTTGCCACAGCCATGGCCAGAGTTCTTCGGGAAACCAAG
CCAATCCCTAACCTCATCTTTGCCATAGAACAGTATGAAAAATTTCTCAT
CCACCTTTCTAAGAAGTCCAAGGTGAACCTGATGCAGCACATGAAGCTCA
GCACCTCACaAGACTTCAAGATCAAAGGAAACATCCTAGACATGGTTCTT
CGAGAGGATGGTGAAGATGAAAATGAAGAGGGCACTGCATCAGAGCATGG
GGGACAGAACAAAGAACCAGCCAAGAAGAAAAGGAAAAAATAAGCGGCCG
C

Amino Acid Sequence

MMEQKLISEEDLGSGSMDQKILSLAAEKTADKLQEFLQTLREGDLTNLLQ
NQAVKGKVAGALLRAIFKGSPCSEEAGTLRRRKIYTCCIQLVESGDLQKE
IASEIIGLLMLEAHHFPGPLLVELANEFISAVREGSLVNGKSLELLPIIL
TALATKKENLAYGKGVLSGEECKKQLINTLCSGRWDQQYVIQLTSMFKDV
PLTAEEVEFVVEKALSMFSKMNLQEIPPLVYQLLVLSSKGSRKSVLEGII
AFFSALDKQHNEEQSGDELLDVVTVPSGELRHVEGTIILHIVFAIKLDYE
LGRELVKHLKVGQQGDSNNNLSPFSIALLLSVTRIQRFQDQVLDLLKTSV
VKSFKDLQLLQGSKFLQNLVPHRSYVSTMILEVVKNSVHSWDHVTQGLVE
LGFILMDSYGPKKVLDGKTIETSPSLSRMPNQHACKLGANILLETFKIHE
MIRQEILEQVLNRVVTRASSPISHFLDLLSNIVMYAPLVLQSCSSKVTEA
FDYLSFLPLQTVQRLLKAVQPLLKVSMSMRDCLILVLRKAMFANQLDARK
SAVAGFLLLLKNFKVLGSLSSSQCSQSLSVSQVHVDVHSHYNSVANETFC
LEIMDSLRRCLSQQADVRLMLYEGFYDVLRRNSQLANSVMQTLLSQLKQF
YEPKPDLLPPLKLEACILTQGDKISLQEPLDYLLCCIQHCLAWYKNTVIP
LQQGEEEEEEEEAFYEDLDDILESITNRMIKSELEDFELDKSADFSQSTS
IGIKNNICAFLVMGVCEVLIEYNFSISSFSKNRFEDILSLFMCYKKLSDI
LNEKAGKAKTKMANKTSDSLLSMKFVSSLLTALFRDSIQSHQESLSVLRS
SNEFMRYAVNVALQKVQQLKETGHVSGPDGQNPEKIFQNLCDITRVLLWR
YTSIPTSVEESGKKEKGKSISLLCLEGLQKIFSAVQQFYQPKIQQFLRAL
DVTDKEGEEREDADVSVTQRTAFQIRQFQRSLLNLLSSQEEDFNSKEALL
LVTVLTSLSKLLEPSSPQFVQMLSWTSKICKENSREDALFCKSLMNLLFS
LHVSYKSPVILLRDLSQDIHGHLGDIDQDVEVEKTNHFAIVNLRTAAPTV
CLLVLSQAEKVLEEVDWLITKLKGQVSQETLSEEASSQATLPNQPVEKAI
IMQLGTLLTFFHELVQTALPSGSCVDTLLKDLCKMYTTLTALVRYYLQVC
QSSGGIPKNMEKLVKLSGSHLTPLCYSFISYVQNKSKSLNYTGEKKEKPA
AVATAMARVLRETKPIPNLIFAIEQYEKFLIHLSKKSKVNLMQHMKLSTS
QDFKIKGNILDMVLREDGEDENEEGTASEHGGQNKEPAKKKRKK*AA

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24040
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All FANCI plasmids MUST be grown at 30 degrees C or less to prevent recombination.