cDNA Clone FAM76b

• Plasmid: pGEX6P Halo.TEV(not blunt) Fam76b F246-D330
• Parent Plasmid: pGEX6P1 Halo TEV
• Expressed: GST-C3-Halo.TEV(not blunt) Fam76b F246-D330
• Genbank: NM_144664.4 GI:134288895
• Tag: GST
• Tag: HALO
• Species: Human

Sequence of Insert

GGATCCTTTGTCCTTATAAGTCAATTGAAAGAAGAAGTGATGTCACTTAAGCGTCTCTTACAGCAGAGAGACCAGACCATTTTAGAAAAAGATAAAAAGTTAACTGAACTAAAGGCAGACTTTCAGTACCAAGAGTCAAATTTGAGAACAAAAATGAACAGTATGGAAAAAGCCCACAAAGAAACTGTGGAACAACTTCAGGCCAAAAACAGAGAACTACTCAAACAGGTCGCAGCATTATCAAAGGGTAAAAAATTTGACTAAGCGGCCCAAGGGCGAATTCCACATTGGTCGCTGCAGCCCGGGGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGENLYFQGGSFVLISQLKEEVMS
LKRLLQQRDQTILEKDKKLTELKADFQYQESNLRTKMNSMEKAHKETVEQ
LQAKNRELLKQVAALSKGKKFD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU45607
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All FAM76b plasmids MUST be grown at 30 degrees C or less to prevent recombination.; Parental: 24005