cDNA Clone NLGN3

• Synonyms: Neuroligin 3
• Plasmid: pCMV NLGN3 HA after aa37 (signal peptide)
• Parent Plasmid: pCMV5D
• Expressed: NLGN3 HA
• Genbank: NM_181303.1
• Tag: HA
• Species: Human

Sequence of Insert

gaattcATGTGGCTGCGGCTTGGCCCGCCCTCGCTGTCCCTGAGCCCCAAGCCCACGGTTGGCAGGAGCCTGTGCCTCACCCTGTGGTTCCTCAGTTTGGCGCTGAGGGCCAGTACCTACCCATACGATGTGCCAGATTACGCCCAGGCCCCAGCACCCACAGTCAACACTCACTTTGGGAAGCTAAGGGGTGCCCGAGTACCACTGCCCAGTGAGATCCTGGGGCCTGTGGACCAATACCTGGGGGTGCCCTACGCAGCTCCCCCGATCGGCGAGAAACGTTTCCTGCCCCCTGAACCACCCCCATCCTGGTCGGGCATCCGGAACGCCACACACTTTCCCCCAGTGTGCCCCCAGAACATCCACACAGCTGTGCCCGAAGTCATGCTGCCGGTCTGGTTCACTGCCAACTTGGATATCGTCGCTACTTACATCCAGGAGCCCAACGAAGACTGTCTCTACCTGAACGTCTATGTGCCGACGGAGGATGTAAAGCGGATTTCCAAGGAATGCGCCCGAAAGCCCAACAAGAAAATTTGTAGGAAAGGAGGATCCGGCGCTAAGAAACAGGGCGAGGACTTAGCGGATAATGACGGGGATGAAGATGAAGACATCCGGGACAGTGGTGCTAAACCCGTCATGGTCTACATCCACGGAGGCTCTTACATGGAAGGGACAGGCAACATGATTGATGGCAGCATCCTCGCCAGTTATGGCAATGTCATCGTCATCACCCTCAACTATCGGGTTGGAGTGCTAGGTTTCCTGAGTACTGGAGATCAGGCTGCCAAGGGCAACTATGGGCTCCTTGACCAGATCCAGGCCCTCCGCTGGGTGAGCGAGAATATTGCCTTCTTCGGGGGAGACCCCCGCCGGATCACTGTCTTTGGCTCGGGCATTGGTGCATCCTGCGTCAGCCTCCTCACGTTGTCACATCACTCAGAGGGACTTTTCCAGAGAGCCATCATCCAAAGTGGCTCTGCTCTGTCCAGCTGGGCTGTGAACTACCAACCAGTGAAGTACACCAGCCTGCTGGCAGACAAAGTGGGCTGTAATGTGCTGGACACCGTGGATATGGTGGACTGTCTTCGGCAAAAGAGTGCCAAGGAGCTGGTAGAGCAGGACATCCAGCCAGCCCGCTACCACGTGGCCTTTGGCCCTGTGATTGATGGTGATGTCATTCCTGATGACCCTGAGATCCTCATGGAGCAGGGCGAGTTCCTCAACTATGACATCATGCTAGGTGTCAACCAGGGCGAGGGTCTCAAGTTTGTGGAAGGGGTGGTGGACCCTGAGGATGGTGTCTCTGGCACTGACTTTGACTATTCCGTCTCCAATTTTGTGGACAATCTGTATGGCTATCCTGAGGGTAAGGACACCCTGCGAGAGACCATCAAGTTCATGTATACAGACTGGGCAGACCGTGACAACCCTGAGACCCGCCGTAAAACACTGGTGGCACTCTTCACTGACCACCAGTGGGTGGAGCCCTCAGTGGTGACAGCCGATCTGCATGCCCGCTACGGCTCGCCTACCTACTTCTACGCCTTCTATCATCACTGCCAGAGCCTCATGAAGCCTGCTTGGTCAGATGCAGCTCATGGGGATGAAGTACCCTATGTTTTTGGGGTTCCTATGGTAGGCCCCACTGACCTTTTCCCCTGCAACTTCTCCAAGAATGATGTTATGCTCAGTGCTGTCGTCATGACCTATTGGACCAACTTTGCCAAGACTGGGGATCCCAACAAGCCGGTCCCCCAGGACACCAAGTTCATTCACACCAAGGCCAACCGCTTTGAGGAAGTGGCCTGGTCCAAATACAATCCCCGAGACCAGCTCTACCTTCACATCGGGCTGAAACCAAGGGTCCGAGATCATTACCGGGCCACTAAGGTGGCCTTTTGGAAACATCTGGTGCCCCACCTATACAACCTGCATGACATGTTCCACTATACGTCCACCACCACCAAAGTGCCGCCTCCGGATACCACCCACAGCTCCCACATCACCCGCAGGCCCAATGGCAAGACCTGGAGCACCAAGCGGCCAGCCATCTCACCTGCCTACAGCAACGAGAATGCCCAGGGGTCCTGGAACGGGGACCAGGATGCAGGGCCACTCCTGGTGGAGAACCCTCGTGACTACTCCACTGAATTAAGTGTCACCATCGCCGTGGGGGCCTCCCTCCTGTTCCTTAACGTTCTGGCCTTCGCTGCCCTCTACTACCGTAAGGACAAACGGCGCCAGGAGCCCCTGCGGCAGCCTAGCCCTCAGCGGGGAGCCGGGGCCCCGGAGTTGGGAGCTGCTCCAGAGGAGGAGCTGGCAGCATTACAACTGGGCCCCACCCACCACGAGTGTGAGGCCGGTCCCCCCCATGACACGCTGCGCCTCACTGCATTGCCCGACTACACCCTGACCCTGCGGCGCTCCCCGGATGACATCCCACTCATGACCCCCAACACCATCACTATGATCCCCAACTCCCTGGTAGGGCTGCAGACATTGCACCCCTATAACACCTTTGCCGCAGGGTTCAACAGTACCGGGCTGCCCCACTCACACTCCACTACCCGGGTATAGgcggccgc

Amino Acid Sequence

EFMWLRLGPPSLSLSPKPTVGRSLCLTLWFLSLALRASTypydvpdyaQA
PAPTVNTHFGKLRGARVPLPSEILGPVDQYLGVPYAAPPIGEKRFLPPEP
PPSWSGIRNATHFPPVCPQNIHTAVPEVMLPVWFTANLDIVATYIQEPNE
DCLYLNVYVPTEDVKRISKECARKPNKKICRKGGSGAKKQGEDLADNDGD
EDEDIRDSGAKPVMVYIHGGSYMEGTGNMIDGSILASYGNVIVITLNYRV
GVLGFLSTGDQAAKGNYGLLDQIQALRWVSENIAFFGGDPRRITVFGSGI
GASCVSLLTLSHHSEGLFQRAIIQSGSALSSWAVNYQPVKYTSLLADKVG
CNVLDTVDMVDCLRQKSAKELVEQDIQPARYHVAFGPVIDGDVIPDDPEI
LMEQGEFLNYDIMLGVNQGEGLKFVEGVVDPEDGVSGTDFDYSVSNFVDN
LYGYPEGKDTLRETIKFMYTDWADRDNPETRRKTLVALFTDHQWVEPSVV
TADLHARYGSPTYFYAFYHHCQSLMKPAWSDAAHGDEVPYVFGVPMVGPT
DLFPCNFSKNDVMLSAVVMTYWTNFAKTGDPNKPVPQDTKFIHTKANRFE
EVAWSKYNPRDQLYLHIGLKPRVRDHYRATKVAFWKHLVPHLYNLHDMFH
YTSTTTKVPPPDTTHSSHITRRPNGKTWSTKRPAISPAYSNENAQGSWNG
DQDAGPLLVENPRDYSTELSVTIAVGASLLFLNVLAFAALYYRKDKRRQE
PLRQPSPQRGAGAPELGAAPEEELAALQLGPTHHECEAGPPHDTLRLTAL
PDYTLTLRRSPDDIPLMTPNTITMIPNSLVGLQTLHPYNTFAAGFNSTGL
PHSHSTTRV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU22999
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Signal Peptide-HA-NLGN3. HA tag inserted after aa37 of NLGN3 (HA in lower case in Protein Sequence); Has 3 silent mutations at positions P103, L297, P629 ; Parental Plasmid DU41497