cDNA Clone DCUN1D1

• Plasmid: pcDNA5-FRT/TO-mCherry- DCUN1D1 D211A A235R D241A
• Parent Plasmid: pcDNA5D FRT/TO mCherry
• Expressed: mCherry-DCUN1D1 D211A A235R D241A
• Tag: mCherry
• Species: Human

Sequence of Insert

ggatccATGAACAAGTTGAAATCATCGCAGAAGGATAAAGTTCGTCAGTT
TATGATCTTCACACAATCTAGTGAAAAAACAGCAGTAAGTTGTCTTTCTC
AAAATGACTGGAAGTTAGATGTTGCAACAGATAATTTTTTCCAAAATCCT
GAACTTTATATACGAGAGAGTGTAAAAGGATCATTGGACAGGAAGAAGTT
AGAACAGCTGTACAATAGATACAAAGACCCTCAAGATGAGAATAAAATTG
GAATAGATGGCATACAGCAGTTCTGTGATGACCTGGCACTCGATCCAGCC
AGCATTAGTGTGTTGATTATTGCATGGAAGTTCAGAGCAGCAACACAGTG
CGAGTTCTCCAAACAGGAGTTCATGGATGGCATGACAGAATTAGGATGTG
ACAGCATAGAAAAACTAAAGGCCCAGATACCCAAGATGGAACAAGAATTG
AAAGAACCAGGACGATTTAAGGATTTTTACCAGTTTACTTTTAATTTTGC
AAAGAATCCAGGACAAAAAGGATTAGATCTAGAAATGGCCATTGCCTACT
GGAACTTAGTGCTTAATGGAAGATTTAAATTCTTAGACTTATGGAATAAA
TTTTTGTTGGAACATCATAAACGATCAATACCAAAAGcCACTTGGAATCT
TCTTTTAGACTTCAGTACGATGATTGCAGATGACATGTCTAATTATGATG
AAGAAGGAagATGGCCTGTTCTTATTGcTGACTTTGTGGAATTTGCACGC
CCTCAAATTGCTGGGACAAAAAGTACAACAGTGTAGGCGGCCGC

Amino Acid Sequence

MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWER
VMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEA
SSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNV
NIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKSGLGSMNKLKSSQK
DKVRQFMIFTQSSEKTAVSCLSQNDWKLDVATDNFFQNPELYIRESVKGS
LDRKKLEQLYNRYKDPQDENKIGIDGIQQFCDDLALDPASISVLIIAWKF
RAATQCEFSKQEFMDGMTELGCDSIEKLKAQIPKMEQELKEPGRFKDFYQ
FTFNFAKNPGQKGLDLEMAIAYWNLVLNGRFKFLDLWNKFLLEHHKRSIP
KATWNLLLDFSTMIADDMSNYDEEGRWPVLIADFVEFARPQIAGTKSTTV
*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24053
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: a632c (D211A), g703a/c704g (A235R) and a722c (D241A). clone also harbours silent substitution g318a (Ala106) compared to NCBI ref