cDNA Clone IKKb

• Plasmid: pRetrox tight HA IKKb S181A
• Parent Plasmid: pRetroX tight puro
• Expressed: HA IKKb S181A
• Genbank: NP_001547.1
• Tag: HA
• Species: Human

Sequence of Insert

gcggccgcgagctggtcaccttccctgacaacgcagacatgtggggcctg
ggaaatgaaagagcgccttgggacagggggatttggaaatgtcatccgat
ggcacaatcaggaaacaggtgagcagattgccatcaagcagtgccggcag
gagctcagcccccggaaccgagagcggtggtgcctggagatccagatcat
gagaaggctgacccaccccaatgtggtggctgcccgagatgtccctgagg
ggatgcagaacttggcgcccaatgacctgcccctgctggccatggagtac
tgccaaggaggagatctccggaagtacctgaaccagtttgagaactgctg
tggtctgcgggaaggtgccatcctcaccttgctgagtgacattgcctctg
cgcttagataccttcatgaaaacagaatcatccatcgggatctaaagcca
gaaaacatcgtcctgcagcaaggagaacagaggttaatacacaaaattat
tgacctaggatatgccaaggagctggatcagggcagtctttgcacagcct
tcgtggggaccctgcagtacctggccccagagctactggagcagcagaag
tacacagtgaccgtcgactactggagcttcggcaccctggcctttgagtg
catcacgggcttccggcccttcctccccaactggcagcccgtgcagtggc
attcaaaagtgcggcagaagagtgaggtggacattgttgttagcgaagac
ttgaatggaacggtgaagttttcaagctctttaccctaccccaataatct
taacagtgtcctggctgagcgactggagaagtggctgcaactgatgctga
tgtggcacccccgacagaggggcacggatcccacgtatgggcccaatggc
tgcttcaaggccctggatgacatcttaaacttaaagctggttcatatctt
gaacatggtcacgggcaccatccacacctaccctgtgacagaggatgaga
gtctgcagagcttgaaggccagaatccaacaggacacgggcatcccagag
gaggaccaggagctgctgcaggaagcgggcctggcgttgatccccgataa
gcctgccactcagtgtatttcagacggcaagttaaatgagggccacacat
tggacatggatcttgtttttctctttgacaacagtaaaatcacctatgag
actcagatctccccacggccccaacctgaaagtgtcagctgtatccttca
agagcccaagaggaatctcgccttcttccagctgaggaaggtgtggggcc
aggtctggcacagcatccagaccctgaaggaagattgcaaccggctgcag
cagggacagcgagccgccatgatgaatctcctccgaaacaacagctgcct
ctccaaaatgaagaattccatggcttccatgtctcagcagctcaaggcca
agttggatttcttcaaaaccagcatccagattgacctggagaagtacagc
gagcaaaccgagtttgggatcacatcagataaactgctgctggcctggag
ggaaatggagcaggctgtggagctctgtgggcgggagaacgaagtgaaac
tcctggtagaacggatgatggctctgcagaccgacattgtggacttacag
aggagccccatgggccggaagcaggggggaacgctggacgacctagagga
gcaagcaagggagctgtacaggagactaagggaaaaacctcgagaccagc
gaactgagggtgacagtcaggaaatggtacggctgctgcttcaggcaatt
cagagcttcgagaagaaagtgcgagtgatctatacgcagctcagtaaaac
tgtggtttgcaagcagaaggcgctggaactgttgcccaaggtggaagagg
tggtgagcttaatgaatgaggatgagaagactgttgtccggctgcaggag
aagcggcagaaggagctctggaatctcctgaagattgcttgtagcaaggt
ccgtggtcctgtcagtggaagcccggatagcatgaatgcctctcgactta
gccagcctgggcagctgatgtctcagccctccacggcctccaacagctta
cctgagccagccaagaagagtgaagaactggtggctgaagcacataacct
ctgcaccctgctagaaaatgccatacaggacactgtgagggaacaagacc
agagtttcacggccctagactggagctggttacagacggaagaagaagag
cacagctgcctggagcaggcctcatgagcggccgc

Amino Acid Sequence

MYPYDVPDYAGSGEFGAAASWSPSLTTQTCGAWEMKERLGTGGFGNVIRW
HNQETGEQIAIKQCRQELSPRNRERWCLEIQIMRRLTHPNVVAARDVPEG
MQNLAPNDLPLLAMEYCQGGDLRKYLNQFENCCGLREGAILTLLSDIASA
LRYLHENRIIHRDLKPENIVLQQGEQRLIHKIIDLGYAKELDQGSLCTAF
VGTLQYLAPELLEQQKYTVTVDYWSFGTLAFECITGFRPFLPNWQPVQWH
SKVRQKSEVDIVVSEDLNGTVKFSSSLPYPNNLNSVLAERLEKWLQLMLM
WHPRQRGTDPTYGPNGCFKALDDILNLKLVHILNMVTGTIHTYPVTEDES
LQSLKARIQQDTGIPEEDQELLQEAGLALIPDKPATQCISDGKLNEGHTL
DMDLVFLFDNSKITYETQISPRPQPESVSCILQEPKRNLAFFQLRKVWGQ
VWHSIQTLKEDCNRLQQGQRAAMMNLLRNNSCLSKMKNSMASMSQQLKAK
LDFFKTSIQIDLEKYSEQTEFGITSDKLLLAWREMEQAVELCGRENEVKL
LVERMMALQTDIVDLQRSPMGRKQGGTLDDLEEQARELYRRLREKPRDQR
TEGDSQEMVRLLLQAIQSFEKKVRVIYTQLSKTVVCKQKALELLPKVEEV
VSLMNEDEKTVVRLQEKRQKELWNLLKIACSKVRGPVSGSPDSMNASRLS
QPGQLMSQPSTASNSLPEPAKKSEELVAEAHNLCTLLENAIQDTVREQDQ
SFTALDWSWLQTEEEEHSCLEQAS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU46052
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: DU43897 inserted and the frame corrected with Mp10432 Mp10433