cDNA Clone DCUN1D1

• Synonyms: (Defective in cullin neddylation 1, domain containing 1)
• Plasmid: pGEX6P-1-DCUN1D1 K171R
• Parent Plasmid: pGEX6P1
• Expressed: GST-DCUN1D1 K171R
• Genbank: NM_020640
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGAACAAGTTGAAATCATCGCAGAAGGATAAAGTTCGTCAGTT
TATGATCTTCACACAATCTAGTGAAAAAACAGCAGTAAGTTGTCTTTCTC
AAAATGACTGGAAGTTAGATGTTGCAACAGATAATTTTTTCCAAAATCCT
GAACTTTATATACGAGAGAGTGTAAAAGGATCATTGGACAGGAAGAAGTT
AGAACAGCTGTACAATAGATACAAAGACCCTCAAGATGAGAATAAAATTG
GAATAGATGGCATACAGCAGTTCTGTGATGACCTGGCACTCGATCCAGCC
AGCATTAGTGTGTTGATTATTGCATGGAAGTTCAGAGCAGCAACACAGTG
CGAGTTCTCCAAACAGGAGTTCATGGATGGCATGACAGAATTAGGATGTG
ACAGCATAGAAAAACTAAAGGCCCAGATACCCAAGATGGAACAAGAATTG
AAAGAACCAGGACGATTTAAGGATTTTTACCAGTTTACTTTTAATTTTGC
AAAGAATCCAGGACAAAGGGGATTAGATCTAGAAATGGCCATTGCCTACT
GGAACTTAGTGCTTAATGGAAGATTTAAATTCTTAGACTTATGGAATAAA
TTTTTGTTGGAACATCATAAACGATCAATACCAAAAGACACTTGGAATCT
TCTTTTAGACTTCAGTACGATGATTGCAGATGACATGTCTAATTATGATG
AAGAAGGAGCATGGCCTGTTCTTATTGATGACTTTGTGGAATTTGCACGC
CCTCAAATTGCTGGGACAAAAAGTACAACAGTGTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMNKLKSSQKDKVRQFMIFT
QSSEKTAVSCLSQNDWKLDVATDNFFQNPELYIRESVKGSLDRKKLEQLY
NRYKDPQDENKIGIDGIQQFCDDLALDPASISVLIIAWKFRAATQCEFSK
QEFMDGMTELGCDSIEKLKAQIPKMEQELKEPGRFKDFYQFTFNFAKNPG
QRGLDLEMAIAYWNLVLNGRFKFLDLWNKFLLEHHKRSIPKDTWNLLLDF
STMIADDMSNYDEEGAWPVLIDDFVEFARPQIAGTKSTTV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU24071
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: carries g318a mutation (silent Ala106)