cDNA Clone IKKbeta S181E

• Plasmid: pCMVHA-2 IKKb S181E
• Parent Plasmid: pCMV5 HA2
• Expressed: HA IKKb S181E
• Genbank: BAI45894.1
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCGATGAGCTGGTCACCTTCCCTGACAACGCAGACATGTGGGGC
CTGGGAAATGAAAGAGCGCCTTGGGACAGGGGGATTTGGAAATGTCATCC
GATGGCACAATCAGGAAACAGGTGAGCAGATTGCCATCAAGCAGTGCCGG
CAGGAGCTCAGCCCCCGGAACCGAGAGCGGTGGTGCCTGGAGATCCAGAT
CATGAGAAGGCTGACCCACCCCAATGTGGTGGCTGCCCGAGATGTCCCTG
AGGGGATGCAGAACTTGGCGCCCAATGACCTGCCCCTGCTGGCCATGGAG
TACTGCCAAGGAGGAGATCTCCGGAAGTACCTGAACCAGTTTGAGAACTG
CTGTGGTCTGCGGGAAGGTGCCATCCTCACCTTGCTGAGTGACATTGCCT
CTGCGCTTAGATACCTTCATGAAAACAGAATCATCCATCGGGATCTAAAG
CCAGAAAACATCGTCCTGCAGCAAGGAGAACAGAGGTTAATACACAAAAT
TATTGACCTAGGATATGCCAAGGAGCTGGATCAGGGCAGTCTTTGCACAg
aATTCGTGGGGACCCTGCAGTACCTGGCCCCAGAGCTACTGGAGCAGCAG
AAGTACACAGTGACCGTCGACTACTGGAGCTTCGGCACCCTGGCCTTTGA
GTGCATCACGGGCTTCCGGCCCTTCCTCCCCAACTGGCAGCCCGTGCAGT
GGCATTCAAAAGTGCGGCAGAAGAGTGAGGTGGACATTGTTGTTAGCGAA
GACTTGAATGGAACGGTGAAGTTTTCAAGCTCTTTACCCTACCCCAATAA
TCTTAACAGTGTCCTGTCTGAGCGACTGGAGAAGTGGCTGCAACTGATGC
TGATGTGGCACCCCCGACAGAGGGGCACGGATCCCACGTATGGGCCCAAT
GGCTGCTTCAAGGCCCTGGATGACATCTTAAACTTAAAGCTGGTTCATAT
CTTGAACATGGTCACGGGCACCATCCACACCTACCCTGTGACAGAGGATG
AGAGTCTGCAGAGCTTGAAGGCCAGAATCCAACAGGACACGGGCATCCCA
GAGGAGGACCAGGAGCTGCTGCAGGAAGCGGGCCTGGCGTTGATCCCCGA
TAAGCCTGCCACTCAGTGTATTTCAGACGGCAAGTTAAATGAGGGCCACA
CATTGGACATGGATCTTGTTTTTCTCTTTGACAACAGTAAAATCACCTAT
GAGACTCAGATCTCCCCACGGCCCCAACCTGAAAGTGTCAGCTGTATCCT
TCAAGAGCCCAAGAGGAATCTCGCCTTCTTCCAGCTGAGGAAGGTGTGGG
GCCAGGTCTGGCACAGCATCCAGACCCTGAAGGAAGATTGCAACCGGCTG
CAGCAGGGACAGCGAGCCGCCATGATGAATCTCCTCCGAAACAACAGCTG
CCTCTCCAAAATGAAGAATTCCATGGCTTCCATGTCTCAGCAGCTCAAGG
CCAAGTTGGATTTCTTCAAAACCAGCATCCAGATTGACCTGGAGAAGTAC
AGCGAGCAAACCGAGTTTGGGATCACATCAGATAAACTGCTGCTGGCCTG
GAGGGAAATGGAGCAGGCTGTGGAGCTCTGTGGGCGGGAGAACGAAGTGA
AACTCCTGGTAGAACGGATGATGGCTCTGCAGACCGACATTGTGGACTTA
CAGAGGAGCCCCATGGGCCGGAAGCAGGGGGGAACGCTGGACGACCTAGA
GGAGCAAGCAAGGGAGCTGTACAGGAGACTAAGGGAAAAACCTCGAGACC
AGCGAACTGAGGGTGACAGTCAGGAAATGGTACGGCTGCTGCTTCAGGCA
ATTCAGAGCTTCGAGAAGAAAGTGCGAGTGATCTATACGCAGCTCAGTAA
AACTGTGGTTTGCAAGCAGAAGGCGCTGGAACTGTTGCCCAAGGTGGAAG
AGGTGGTGAGCTTAATGAATGAGGATGAGAAGACTGTTGTCCGGCTGCAG
GAGAAGCGGCAGAAGGAGCTCTGGAATCTCCTGAAGATTGCTTGTAGCAA
GGTCCGTGGTCCTGTCAGTGGAAGCCCGGATAGCATGAATGCCTCTCGAC
TTAGCCAGCCTGGGCAGCTGATGTCTCAGCCCTCCACGGCCTCCAACAGC
TTACCTGAGCCAGCCAAGAAGAGTGAAGAACTGGTGGCTGAAGCACATAA
CCTCTGCACCCTGCTAGAAAATGCCATACAGGACACTGTGAGGGAACAAG
ACCAGAGTTTCACGGCCCTAGACTGGAGCTGGTTACAGACGGAAGAAGAA
GAGCACAGCTGCCTGGAGCAGGCCTCATGATGTGGGGGGACTCGACCCCC
TGACATGGGGCAGCCCATAGCAGGCCTTGTGCGGTGGGGGGACTCGACCC
CCTGACATGGGGCTGCCTGGAGCAGGCCTCATGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAMSWSPSLTTQTCGAWEMKERLGTGGFG
NVIRWHNQETGEQIAIKQCRQELSPRNRERWCLEIQIMRRLTHPNVVAAR
DVPEGMQNLAPNDLPLLAMEYCQGGDLRKYLNQFENCCGLREGAILTLLS
DIASALRYLHENRIIHRDLKPENIVLQQGEQRLIHKIIDLGYAKELDQGS
LCTEFVGTLQYLAPELLEQQKYTVTVDYWSFGTLAFECITGFRPFLPNWQ
PVQWHSKVRQKSEVDIVVSEDLNGTVKFSSSLPYPNNLNSVLSERLEKWL
QLMLMWHPRQRGTDPTYGPNGCFKALDDILNLKLVHILNMVTGTIHTYPV
TEDESLQSLKARIQQDTGIPEEDQELLQEAGLALIPDKPATQCISDGKLN
EGHTLDMDLVFLFDNSKITYETQISPRPQPESVSCILQEPKRNLAFFQLR
KVWGQVWHSIQTLKEDCNRLQQGQRAAMMNLLRNNSCLSKMKNSMASMSQ
QLKAKLDFFKTSIQIDLEKYSEQTEFGITSDKLLLAWREMEQAVELCGRE
NEVKLLVERMMALQTDIVDLQRSPMGRKQGGTLDDLEEQARELYRRLREK
PRDQRTEGDSQEMVRLLLQAIQSFEKKVRVIYTQLSKTVVCKQKALELLP
KVEEVVSLMNEDEKTVVRLQEKRQKELWNLLKIACSKVRGPVSGSPDSMN
ASRLSQPGQLMSQPSTASNSLPEPAKKSEELVAEAHNLCTLLENAIQDTV
REQDQSFTALDWSWLQTEEEEHSCLEQAS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU46010
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: single mutation for transient transfection