cDNA Clone ARIH1

• Synonyms: ARI, HARI, HHARI, UBCH7BP
• Plasmid: pET15 6HIS SUMO ARIH1 T177-N393 C357A (ecoli optimised)
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6His-SUMO-ARIH1 T177-N393 C357A (ecoli optimised)
• Genbank: E coli optimized gene
• Tag: 6His
• Tag: SUMO
• Species: Human

Sequence of Insert

ACCCGTAGCAGCGCACAGGATATGCCGTGTCAGATTTGTTATCTGAATTA
TCCGAACAGCTATTTCACCGGTCTGGAATGTGGTCACAAATTTTGTATGC
AGTGTTGGAGCGAATATCTGACCACCAAAATCATGGAAGAAGGTATGGGT
CAGACCATTAGCTGTCCGGCACATGGTTGTGATATTCTGGTTGATGATAA
TACCGTGATGCGTCTGATTACCGATAGCAAAGTGAAACTGAAATATCAGC
ATCTGATCACCAACAGCTTCGTGGAATGTAATCGTCTGCTGAAATGGTGT
CCTGCACCGGATTGTCATCATGTTGTTAAAGTTCAGTATCCGGATGCAAA
ACCGGTTCGTTGTAAATGTGGTCGTCAGTTTTGTTTTAACTGCGGTGAAA
ATTGGCATGACCCTGTTAAATGTAAATGGCTGAAAAAATGGATCAAAAAA
TGCGACGATGATAGCGAAACCAGCAATTGGATTGCAGCAAATACCAAAGA
ATGTCCGAAATGTCATGTGACCATTGAAAAAGATGGTGGCgcCAATCATA
TGGTTTGCCGTAATCAGAATTGCAAAGCCGAATTTTGTTGGGTTTGTCTG
GGTCCGTGGGAACCGCATGGTAGCGCATGGTATAATTGTAATCGCTATAA
Ttaagcggccgc

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGTRSSAQDMPCQICYLNYPNSYFTGLECGHKFCMQCWSEYLTTKIME
EGMGQTISCPAHGCDILVDDNTVMRLITDSKVKLKYQHLITNSFVECNRL
LKWCPAPDCHHVVKVQYPDAKPVRCKCGRQFCFNCGENWHDPVKCKWLKK
WIKKCDDDSETSNWIAANTKECPKCHVTIEKDGGANHMVCRNQNCKAEFC
WVCLGPWEPHGSAWYNCNRYN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU24103
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Linker sequence deleted after SENP cleavage silent mutation t270c compared to NCBI ref; Parental Plasmid DU32993