cDNA Clone CHK2

• Synonyms: CHEK2
• Plasmid: pCMV-HA CHK2 D347A
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-CHK2 D347A
• Genbank: NM_007194
• Tag: HA
• Species: Human

Sequence of Insert

gaattcatgtctcgggagtcggatgttgaggctcagcagtctcatggcag
cagtgcctgttcacagccccatggcagcgttacccagtcccaaggctcct
cctcacagtcccagggcatatccagctcctctaccagcacgatgccaaac
tccagccagtcctctcactccagctctgggacactgagctccttagagac
agtgtccactcaggaactctattctattcctgaggaccaagaacctgagg
accaagaacctgaggagcctacccctgccccctgggctcgattatgggcc
cttcaggatggatttgccaatcttgaatgtgtgaatgacaactactggtt
tgggagggacaaaagctgtgaatattgctttgatgaaccactgctgaaaa
gaacagataaataccgaacatacagcaagaaacactttcggattttcagg
gaagtgggtcctaaaaactcttacattgcatacatagaagatcacagtgg
caatggaacctttgtaaatacagagcttgtagggaaaggaaaacgccgtc
ctttgaataacaattctgaaattgcactgtcactaagcagaaataaagtt
tttgtcttttttgatctgactgtagatgatcagtcagtttatcctaaggc
attaagagatgaatacatcatgtcaaaaactcttggaagtggtgcctgtg
gagaggtaaagctggctttcgagaggaaaacatgtaagaaagtagccata
aagatcatcagcaaaaggaagtttgctattggttcagcaagagaggcaga
cccagctctcaatgttgaaacagaaatagaaattttgaaaaagctaaatc
atccttgcatcatcaagattaaaaacttttttgatgcagaagattattat
attgttttggaattgatggaagggggagagctgtttgacaaagtggtggg
gaataaacgcctgaaagaagctacctgcaagctctatttttaccagatgc
tcttggctgtgcagtaccttcatgaaaacggtattatacaccgtgcctta
aagccagagaatgttttactgtcatctcaagaagaggactgtcttataaa
gattactgattttgggcactccaagattttgggagagacctctctcatga
gaaccttatgtggaacccccacctacttggcgcctgaagttcttgtttct
gttgggactgctgggtataaccgtgctgtggactgctggagtttaggagt
tattctttttatctgccttagtgggtatccacctttctctgagcatagga
ctcaagtgtcactgaaggatcagatcaccagtggaaaatacaacttcatt
cctgaagtctgggcagaagtctcagagaaagctctggaccttgtcaagaa
gttgttggtagtggatccaaaggcacgttttacgacagaagaagccttaa
gacacccgtggcttcaggatgaagacatgaagagaaagtttcaagatctt
ctgtctgaggaaaatgaatccacagctctaccccaggttctagcccagcc
ttctactagtcgaaagcggccccgtgaaggggaagccgagggtgccgaga
ccacaaagcgcccagctgtgtgtgctgctgtgttgtgagcggccgc

Amino Acid Sequence

MYPYDVPDYAGSPEFMSRESDVEAQQSHGSSACSQPHGSVTQSQGSSSQS
QGISSSSTSTMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQEPEDQEP
EEPTPAPWARLWALQDGFANLECVNDNYWFGRDKSCEYCFDEPLLKRTDK
YRTYSKKHFRIFREVGPKNSYIAYIEDHSGNGTFVNTELVGKGKRRPLNN
NSEIALSLSRNKVFVFFDLTVDDQSVYPKALRDEYIMSKTLGSGACGEVK
LAFERKTCKKVAIKIISKRKFAIGSAREADPALNVETEIEILKKLNHPCI
IKIKNFFDAEDYYIVLELMEGGELFDKVVGNKRLKEATCKLYFYQMLLAV
QYLHENGIIHRALKPENVLLSSQEEDCLIKITDFGHSKILGETSLMRTLC
GTPTYLAPEVLVSVGTAGYNRAVDCWSLGVILFICLSGYPPFSEHRTQVS
LKDQITSGKYNFIPEVWAEVSEKALDLVKKLLVVDPKARFTTEEALRHPW
LQDEDMKRKFQDLLSEENESTALPQVLAQPSTSRKRPREGEAEGAETTKR
PAVCAAVL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11111
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 62645 Daltons