cDNA Clone C9orf84 tv2

• Plasmid: pcDNA5 FRT/TO FLAG C9orf84 tv2 E856A R857A
• Parent Plasmid: pcDNA5 FRT/TO FLAG
• Expressed: FLAG-C9orf84 tv2 E856A R857A
• Genbank: NM_001080551.1 GI:124028518 with t237c (silent to kill BamH1)
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGTCAGAGACTCTGGGGGATGAATTGGAGATATTAAGAGGTAAAATGATGCAGAGAAGACCAAGGTCAGCAGAAGTCAAATACTTCTATTTCTTTAAAATTTTGCTTAGGCTACGCCTGGCTATTTTGAAGTATTTATTTATTGATGATAAAGGAATACTTTTTGTAAGTAGTAGAAAACACCTACCAACTTTGCCTACTCTCTTGAGTAGACTAAAACTGTTTTTGGTAAAGGACCCTCTTTTAGATTTCAAAGGACAGATCTTCACAGAAGCTAATTTTTCCAGGGAATGTTTCTCTCTTCAAGAAACTTTGGAAGCTTTTGTGAAAGAAGATTTTTGTATGGATAAAGTGAACTTTTGTCAAGAGAAACTAGAAGATACAATATGTTTAAATGAGCCGTCAAGTTTTCTTATTGAGTATGAATTCTTAATACCTCCAAGCCTCAAACCAGAAATTGATATTCCATCACTCTCAGAACTGAAGGAGTTATTAAACCCAGTGCCAGAAATAATAAACTATGTAGATGAAAAGGAAAAGCTTTTTGAAAGAGATCTTACTAACAAGCATGGAATTGAGGATATCGGGGATATAAAATTCAGCTCCACAGAGATTTTGACCATTCAAAGCCAGAGTGAACCAGAAGAGTGCAGTAAACCAGGAGAGTTAGAAATGCCACTAACTCCTCTATTCCTAACATGCCAACATTCTTCAGTGAATTCATTACGTACAGAACTTCAGACATTTCCATTATCTCCGGTTTGTAAAATTAATTTGCTTACTGCTGAAGAATCAGCTAATGAATACTACATGATGTGGCAATTAGAAAGATGTAGAAGCCCTTTGAACCCATTTTTGCTTACAGTGCCAAGAATTCAAGAGCCCCACAGCCAATATTCAGTTACAGATTTGAAAAAGATATTTTCTGTTAAAGAAGAAAGCCTTGTGATTAATCTGGAAAAGGCAGAGTGGTGGAAACAAGCAGGACTAAATCTGAAAATGATGGAAACATTGGAACATCTGAATACATATTTATGTCATGATAATTTGTCTTCTAATGACACTAAAATTGAGATATTTTTGCCTACGAAAGTGCTTCAATTAGAATCATGTCTAGAACATAAAAGTCATTCTTCACCTATTGCACTTATTGATGAAAAATCTACAAATGCTCATTTATCACTTCCACAAAAGAGTCCATCTCTGGCAAAAGAAGTACCAGATCTATGTTTTTCTGATGACTATTTCTCTGATAAAGGAGCAGCAAAAGAAGAAAAACCAAAGAATGACCAAGAACCAGTAAACAGAATAATCCAAAAGAAAGAAAATAACGATCACTTTGAACTTGACTGCACAGGACCATCTATTAAATCACCTTCCTCTTCAATAATTAAAAAAGCATCTTTTGAACATGGCAAAAAACAAGAGAATGATTTGGACCTTTTGAGCGACTTTATTATGCTGCGAAATAAATATAAGACTTGCACCTCAAAGACTGAAGTCACAAACAGTGATGAAAAACATGATAAAGAAGCATGTTCTTTGACACTTCAAGAAGAAAGTCCTATTGTTCATATTAATAAAACCCTGGAGGAAATAAATCAGGAAAGGGGAACAGATAGTGTCATTGAAATTCAAGCGTCAGATAGCCAGTGCCAAGCATTTTGCCTCCTCGAAGCAGCAGCTTCTCCTATCTTAAAAAACCTTGTATCCTTGTGTACCCTCCCTACTGCTAATTGGAAATTTGCCACTGTTATTTTTGACCAAACAAGGTTTCTCTTAAAGGAACAAGAAAAAGTAGTAAGTGATGCTGTTCGCCAAGGTACAATTGATGAAAGAGAAATGACTTTCAAGCATGCCGCTCTCTTACATCTTCTGGTAACAATTAGAGATGTCCTTTTAACATGCAGCTTGGACACAGCATTGGGATATTTGTCGAAGGCAAAAGATATCTACAACAGCATTTTAGGCCCCTATTTGGGTGACATTTGGAGACAGCTGGAGATTGTACAGTTTATTAGGGGGAAAAAGCCTGAAACCAACTACAAGATACAAGAATTGCAATGTCAGATACTAAGTTGGATGCAAAGTCAACAGCAAATTAAGGTACTGATTATAATAAGAATGGACTCAGACGGTGAAAAACATTTTCTCATTAAAATTCTTAACAAAATAGAAGGTTTAACACTGACTGTCCTTCATTCAAATGAAAGAAAAGATTTTCTGGAATCTGAAGGTGTTTTAAGGGGTACAAGTTCCTGTGTAGTTGTACATAATCAATATATTGGAGCAGATTTCCCCTGGAGTAATTTCTCATTTGTGGTGGAATACAATTATGTGGAAGACTCTTGTTGGACTAAACACTGCAAAGAGTTGAATATTCCTTACATGGCCTTTAAAGTGATTCTTCCAGACACAGTTTTAGAAAGAAGCACCTTGCTGGATAGATTTGGAGGTTTTCTTTTGGAAATTCAGATTCCATATGTGTTTTTTGCATCTGAAGGACTTCTTAATACTCCAGACATACTTCAGCTGCTAGAATCCAACTATAACATCTCACTAGTAGCCGCTGGCTGCAGTGAGTCATTGAAACTCTTTGGAAGTTCAGAGTGTTATGTAGTGGTGACAATTGATGAACACACTGCCATAATTTTGCAGGATCTAGAAGAATTGAATTATGAGAAGGCATCAGACAATATCATTATGAGGCTGATGGCATTATCATTACAGTACAGATATTGTTGGATAATTTTATATACCAAAGAAACATTAAATTCAGAGTATCTGCTTACAGAAAAGACACTTCATCACCTAGCACTGATTTATGCAGCTTTGGTTTCATTTGGGCTAAACTCTGAAGAACTGGATGTAAAGCTTATAATTGCCCCAGGAGTAGAAGCAACTGCCTTGATAATTCGACAAATTGCTGACCACAGTTTAATGACCTCAAAGAGAGATCCTCATGAATGGTTGGATAAATCCTGGCTTAAAGTTTCACCATCTGAGGAAGAAATGTACTTACTTGATTTTCCATGTATTAACCCATTGGTGGCTCAGCTCATGCTAAATAAAGGACCTTCACTGCATTGGATATTATTAGCAACTCTGTGTCAACTTCAGGAACTCCTACCTGAAGTCCCAGAAAAAGTGTTAAAGCATTTTTGTAGCATCACTTCCCTATTCAAGATTGGTTCTTCTTCCATAACAAAATCACCGCAAATTTCGTCACCTCAGGAAAATAGGAATCAGATTAGTACCTTGTCTTCTCAAAGTTCAGCTTCTGATTTAGACTCTGTCATTCAAGAACATAATGAATATTATCAGTATTTAGGATTAGGAGAGACAGTGCAGGAAGACAAAACCACCATTTTGAATGACAACTCTTCCATTATGGAACTAAAAGAAATCTCAAGTTTTTTACCACCTGTGACTTCATACAATCAGACCAGCTACTGGAAAGACTCCAGCTGTAAATCTAATATAGGGCAGAATACTCCTTTTCTAATTAATATAGAATCAAGGAGACCGGCTTATAACTCCTTTCTAAACCACAGTGATTCAGAGTCAGATGTCTTTTCTTTGGGTCTAACACAAATGAACTGTGAAACTATAAAATCACCAACTGACACTCAGAAGAGAGTGTCAGTTGTCCCCCGTTTTATAAATTCTCAGAAAAGGAGAACACATGAAGCAAAAGGTTTCATAAATAAAGATGTATCGGACCCTATCTTTTCACTAGAGGGCACTCAATCTCCTCTTCATTGGAACTTTAAGAAAAATATATGGGAACAAGAGAATCACCCGTTCAACTTACAATATGGTGCACAGCAGACTGCATGTAACAAATTGTACTCTCAGAAAGGTAATTTATTCACTGATCAGCAAAAATGTCTATCAGATGAGTCTGAAGGCCTCACATGTGAAAGTTCAAAAGATGAGACTTTCTGGAGAGAATTACCATCTGTCCCCAGTTTGGATTTATTTCGTGCTTCTGATTCTAATGCAAATCAAAAAGAATTCAACAGCCTTTATTTCTACCAAAGAGCTGGAAAAAGTTTAGGACAGAAAAGGCACCATGAATCTTCATTTAACTCAGGAGACAAGGAATCATTAACAGGTTTTATGTGCTCACAACTACCACAATTCAAAAAACGACGTCTAGCATATGAAAAAGTCCCTGGTAGAGTTGATGGGCAGACTCGGCTGAGGTTTTTTTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMSETLGDELEILRGKMMQRRPRSAEVKYFYFFKILLRLR
LAILKYLFIDDKGILFVSSRKHLPTLPTLLSRLKLFLVKDPLLDFKGQIF
TEANFSRECFSLQETLEAFVKEDFCMDKVNFCQEKLEDTICLNEPSSFLI
EYEFLIPPSLKPEIDIPSLSELKELLNPVPEIINYVDEKEKLFERDLTNK
HGIEDIGDIKFSSTEILTIQSQSEPEECSKPGELEMPLTPLFLTCQHSSV
NSLRTELQTFPLSPVCKINLLTAEESANEYYMMWQLERCRSPLNPFLLTV
PRIQEPHSQYSVTDLKKIFSVKEESLVINLEKAEWWKQAGLNLKMMETLE
HLNTYLCHDNLSSNDTKIEIFLPTKVLQLESCLEHKSHSSPIALIDEKST
NAHLSLPQKSPSLAKEVPDLCFSDDYFSDKGAAKEEKPKNDQEPVNRIIQ
KKENNDHFELDCTGPSIKSPSSSIIKKASFEHGKKQENDLDLLSDFIMLR
NKYKTCTSKTEVTNSDEKHDKEACSLTLQEESPIVHINKTLEEINQERGT
DSVIEIQASDSQCQAFCLLEAAASPILKNLVSLCTLPTANWKFATVIFDQ
TRFLLKEQEKVVSDAVRQGTIDEREMTFKHAALLHLLVTIRDVLLTCSLD
TALGYLSKAKDIYNSILGPYLGDIWRQLEIVQFIRGKKPETNYKIQELQC
QILSWMQSQQQIKVLIIIRMDSDGEKHFLIKILNKIEGLTLTVLHSNERK
DFLESEGVLRGTSSCVVVHNQYIGADFPWSNFSFVVEYNYVEDSCWTKHC
KELNIPYMAFKVILPDTVLERSTLLDRFGGFLLEIQIPYVFFASEGLLNT
PDILQLLESNYNISLVAAGCSESLKLFGSSECYVVVTIDEHTAIILQDLE
ELNYEKASDNIIMRLMALSLQYRYCWIILYTKETLNSEYLLTEKTLHHLA
LIYAALVSFGLNSEELDVKLIIAPGVEATALIIRQIADHSLMTSKRDPHE
WLDKSWLKVSPSEEEMYLLDFPCINPLVAQLMLNKGPSLHWILLATLCQL
QELLPEVPEKVLKHFCSITSLFKIGSSSITKSPQISSPQENRNQISTLSS
QSSASDLDSVIQEHNEYYQYLGLGETVQEDKTTILNDNSSIMELKEISSF
LPPVTSYNQTSYWKDSSCKSNIGQNTPFLINIESRRPAYNSFLNHSDSES
DVFSLGLTQMNCETIKSPTDTQKRVSVVPRFINSQKRRTHEAKGFINKDV
SDPIFSLEGTQSPLHWNFKKNIWEQENHPFNLQYGAQQTACNKLYSQKGN
LFTDQQKCLSDESEGLTCESSKDETFWRELPSVPSLDLFRASDSNANQKE
FNSLYFYQRAGKSLGQKRHHESSFNSGDKESLTGFMCSQLPQFKKRRLAY
EKVPGRVDGQTRLRFF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU45835
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Recommend growth at low temperature.