cDNA Clone KIAA2022

• Plasmid: pcDNA5 FRT/TO FLAG-NLS KIAA2022 L281A V283A L285A
• Parent Plasmid: pcDNA5 FRT/TO FLAG
• Expressed: FLAG-NLS-KIAA2022 L281A V283A L285A
• Genbank: NM_001008537.2 with silent changes to killinternal BamH1 sites
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCCCACCGAAGAAGAAGCGCAAGGTCATGGATAACCAACAAGATAAGGCTATTGTTGCCTCAGCCAACGGAGAAAACACTCTGATTAATGGGGTCAAAGAAAATGACTCAGAGGACCAGGATGTGGCAATGAAGTCATTTGCAGCTCTAGAAGCTGCTGCACCTATCCAGCCTACACCGGTGGCACAAAAAGAGACCCTGATGTATCCCAGAGGTCTCCTGCCTCTACCCTCTAAGAAGCCCTGTATGCAGAGCCCGCCCTCTCCTTTGGGCCTGATTGAAGCACCCGAACATGCTGCTAATAGTGCTTCTGTGAATGCCATCTCCCTCACATCTGGCATTGCAAAAGGCCTGAACACATGGTCACTTCCCAATGAATGTGAGAAAGCTCCATTTGCCATAATGGAGCCTGCAGGCATGTCAGCTCTGAATGGGGACTGTCTCATGCAGCCAAGTCGGACTTGCTTAGGCTGCTTCATGGAATCCAAGGATGCAGTAGATCCTGAGCCAGGGATCAGTCTGAAAGTTGGTGATCTAAATAGGGATTATGAAACGTGTGCAGTCTCTGATATAGGGATTCAGTGTATTAATGCTGGAGAAAATATGAAATATGGAGAGCAGCTGCTCTCAGACCAGCTCCTAGGCTTCCCCCTGCATAAGTCAAGGGCAGGAGACAGACGAGAAACTGAGAAACCTGACATTGACTTGGAGGACCCGGCTCAGAAAAGCTATTATGAGGCATTACTGTTAGACAAGTGCAATACAGAAGAAGCTTTGCTTGCAAATTCCAATCAGGATTGGGGTTACTTCGAGACTTTTATTAGTGAAAGTAAGATTGAACTGCTTGACCTCTGTTCCAAAAATGAGGCCTCTGCTAACGCCTTCTCTGAAGAAGATGTGGATAACTACATGTTTGATGATGATGAATCAACACTAGGCAGTGATGTCTGCTCCCTGAAAATTCGATATGAATCCTTTCAGGACAATGTTCGAGACAAGACTACTCTTTTGATGCAGGAAGATGCCCAATTCAACTTTTTTCCCAGCGTCTTTACTACCTGCCCCAAGCGAGAGTCTAAGAGTGGGGCCCTGAAGCAGAGCAGTGATTTTTCCCAATTCAAGGTCCCTGATGTGAGCATCATCTGGGGGGAGGAAGATAAAAACTTGGACAAGAAGAAAGGCAAAGAGGAAGGACAGGAAGACAAAGGTGTAGAGAAGAAAGATGGAAAGGATAATGGAGAAAAGCCTGCCTTAAATAAACCATGCAGTGGGACTGAAGTAGAGCAACTTAAGAATCCAAAGCAGGGCCATCTTGCTAATTCCCTGGAGACATCAGGGAGTTTCAGTGATGATAGTTCCTTCATTGAGATCTCATATGATGCTATGGGTGAGATCAAGGACTGTAGTCGCTATATGGCTCGGGACACTAATTCTGGCAGCTCCTCCTCCCAACAGAACTATGGGCTGCGAGCCAAGAGAAAAGTCAGATACAGTGAAGATTATCTATATGATGTTGACTCACTAGAGGGTGAAAAAGTAAATGAGAGGAAGGAATGGCTGCCAGTTGGTTCCAAAGAGGAAGATGATGATGAATGGTGTCCCAAAAAGAGAAGAAAAGTAACCCGTAAGGAGCCCCCTGTTATTATCAAATATATCATCATTAATCGCTTTAAAGGTGAGAAGAACATGCTGGTGAAGTTGGGTAAGGTGGATGCCAGTGAGACAACAGTGAATTTGAGTGAGAATCAGCTCAACAAATATGCCAAGCTGGCACCCTTGAAAGGCTTCTGGCAAAAGAAGAAGAAGCAGAGAAACACCAACACGGACTCCATCAAGACACCTTTTTCCCAAAAGCAAAGCTTTGAACCAGGTAGCTTTGAGGTGTCATTTCTGCCACCTGCTCGCAAACGAAAATCTAAACTTGGCAACAGGCACAGGATTCAAAGAATCCCATCCATTGAAATTTCAGCAAGTAGTAAACAGATTTCATTATGTAATGATCAGAGGCACGCTAGTAATCATAAAGAAGATGGAGGCCTAAAAGGTACACTGAAGTCAGCACCTCTGGGTGCTCCTAGCTGTGCAAATGGATCACATTTAAATGACATCACAGGCCCTGACTCAGTGAAAGTCAAAGCCCAAGACACAGAGTTTAAGGGGCCAGAGAGGAAAGTGCTCAATAAAATCAAATTTAAAAGTGAAGCTAGGTTAAAATCTAAGAAAGTCAAAGCTGCTGGGCAAGAAAGCAAGCCAATTGTTCAAATGAGCCCTCTCTTGGAAAACCAATCCTCAAAGGCTAATTTAAAGAATGAAGTTATTCCTGGGACATCAAACAGTTCCCGTCTATCTGAATTTCATGAGGCAAAGGCTGCTAAGAGTTCCACTTTTCTACCAACGACATGCTCTTCTGAAATGCCTTTATCATCTGCTAATGTTACCACTAATATACCTGTTATCCCGGGAGGGTATCTGCAGACATTGTTAGATGCTTCTGACTTGTCAAATAACACTAGTATCTCATACTTCAGCCACCATTCTCCAGAGCAAAATGAAGGCAGCCTCACTCAAACCGAAAAATCATTTGTACCCCTCCAGCCTACCCAGGACTGTGTGCTCACCTCATCCTCTGACTCTGAGCTGCAGCAGTCATCTCATAACTTCAAAATGGAATCAAGCAACTATAGAAATGTGTGGCCCAACAAGGCTACTTCTGGCACCCAGGAATTCATGGCTGAAGTCTCAAGGGAGATAGCCCCAACCCAATCCAGTGAATTTGGAGCCTCCCAAGTAGTCTCCATGGAAAATAACCTCACACCTACAACATACAATCCAATCTGTCTCAATAGTGGTGGCAGTAATTGCAACAAGGTCCTGTATGACTCCATGCAAGATACCCAACTCCCATCTGATGACTCTTACCAATTATGTCACTTTAATAATGGAGAGATCTGCTTTCCTTTCCAGCAGGGGCCAGTCAATATGGATGATGGTCGGCTCTTTAGCTTTGATTCAATGGCCCCACTCTCTGTCAGCTCAAGTAATTATTGCTCCTTAAGCTTGAAGTCCTGTGAAAAGGATGGCGATGATGATATCACTGATGACTTCCTGGCCCATTGCAGCCCTAAGCTGGTGATCCAGCAAAGCATTGATGAGATAGCACCACTAAAGGAGTCCACTGACCTCCTGGATATATCCAACTTCACCCCTGACAAATTCCGCCACTCTTCCCTTTCAGAGATGTCCCCACCGGACACCCCTAGTCTTTCCCCTCAAATTACCAGATGTGAGAGTATGAAGACACTAGGAACACTAAAGGGGTTCCAAGAGGGTGTCCCAGGACCATTGGACAGTGTGGAAAAAATCAAGTGGGACTGCAGTACCCTTTCACGGCAGGTCCAAATGGAGGATGGATTTACTTTAAATAATCACCAGTTTCAGTTCCATATGTTCAATGATGAGGATTCTGTCAGCCTGCTCCAAAAAAACCCTTGCCTGTCAACATTTAATGATCCATCTGGTCAAATTAGTACCAACAACAAAGTGTCAAAATCAAGAAAGAAAAGTTCACCCAGCAAGAGTGGGGCTATGAACCAAAGCTCTTCTCAGAAAAACACCAGGAAAAAATCCCTCAAAGGTAACAACAAGGGGATTGAAAAACCACCTGGCAAAAACTCCCGCCAGGTCCCTAAGTCCACAAAGAAAGGGAAATACATGGCTGCCATCAATGGAGAGAAAATGCAAATTGGCATTGGCCGTGGGGGAAGCCAAACCAACACCATATCCTCCACTGGGAAGACATTGGCTGAATGTATCCAACATGGTGGCCCTATGGCCTCTATGAAGATGCCAAGTCAAAAGGGACTTTCTGGAGATTGGGCCTTGGGGAAGGAGAGCAGCCCAGGCTGGAGTGATATGAGCATGGGCACCAACACCAACAGCCTTCTGGATGATGACCAACGGGAATTTCAGGAGCCTTCCTATATCTTGTCCAACATTGCCTCTGGTATGGCAGATGTGCAGAGATTCATGATGGCCTCCATAGAGCCCCTTTGGGAACCCATGGAGCACCATGGAGATCCCAACATATTCTACTCCCCTGAGTCCAATAGTCTAAAATTAAAAACCCTCAAAATATTGGCTGGGACACCACAGGAGTCTAAGAAAAAGATCAACAGTGGGTCCCAAGGAGCCACTAAGAATCACAGGTCAATCAAGGGTGTGAGCAAAAGCAATGGGAAAACAGCAATAGGTGATCCTGGTCGTGCAAACATGCCTGGTTATAACGAGGACTCTCGCTCTACCTTCTTTGATAAAAAGTATAGTAACATGAGCACTTTAGGCAATAACGGACCGACACACAAAAAGTTGTATCGTCACAAATCCAGCTCCAAGGCCCTGAGAGATGAGAAATGTAAGGGAAAGCACATGGAGCGAGAACAGGTCCACAAGGATGAGTCTGGGACAGCTTCTTTTGAAAAACTGAGGGATTCCGACTACAATCTCCTAAAAGCAGAAACAACCTTTTGGGTTTTACCTGTGTTTGAAGAAGAGACTCGCATTTTCCAGAAAGACATTTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSPPKKKRKVMDNQQDKAIVASANGENTLINGVKENDSEDQ
DVAMKSFAALEAAAPIQPTPVAQKETLMYPRGLLPLPSKKPCMQSPPSPL
GLIEAPEHAANSASVNAISLTSGIAKGLNTWSLPNECEKAPFAIMEPAGM
SALNGDCLMQPSRTCLGCFMESKDAVDPEPGISLKVGDLNRDYETCAVSD
IGIQCINAGENMKYGEQLLSDQLLGFPLHKSRAGDRRETEKPDIDLEDPA
QKSYYEALLLDKCNTEEALLANSNQDWGYFETFISESKIELLDLCSKNEA
SANAFSEEDVDNYMFDDDESTLGSDVCSLKIRYESFQDNVRDKTTLLMQE
DAQFNFFPSVFTTCPKRESKSGALKQSSDFSQFKVPDVSIIWGEEDKNLD
KKKGKEEGQEDKGVEKKDGKDNGEKPALNKPCSGTEVEQLKNPKQGHLAN
SLETSGSFSDDSSFIEISYDAMGEIKDCSRYMARDTNSGSSSSQQNYGLR
AKRKVRYSEDYLYDVDSLEGEKVNERKEWLPVGSKEEDDDEWCPKKRRKV
TRKEPPVIIKYIIINRFKGEKNMLVKLGKVDASETTVNLSENQLNKYAKL
APLKGFWQKKKKQRNTNTDSIKTPFSQKQSFEPGSFEVSFLPPARKRKSK
LGNRHRIQRIPSIEISASSKQISLCNDQRHASNHKEDGGLKGTLKSAPLG
APSCANGSHLNDITGPDSVKVKAQDTEFKGPERKVLNKIKFKSEARLKSK
KVKAAGQESKPIVQMSPLLENQSSKANLKNEVIPGTSNSSRLSEFHEAKA
AKSSTFLPTTCSSEMPLSSANVTTNIPVIPGGYLQTLLDASDLSNNTSIS
YFSHHSPEQNEGSLTQTEKSFVPLQPTQDCVLTSSSDSELQQSSHNFKME
SSNYRNVWPNKATSGTQEFMAEVSREIAPTQSSEFGASQVVSMENNLTPT
TYNPICLNSGGSNCNKVLYDSMQDTQLPSDDSYQLCHFNNGEICFPFQQG
PVNMDDGRLFSFDSMAPLSVSSSNYCSLSLKSCEKDGDDDITDDFLAHCS
PKLVIQQSIDEIAPLKESTDLLDISNFTPDKFRHSSLSEMSPPDTPSLSP
QITRCESMKTLGTLKGFQEGVPGPLDSVEKIKWDCSTLSRQVQMEDGFTL
NNHQFQFHMFNDEDSVSLLQKNPCLSTFNDPSGQISTNNKVSKSRKKSSP
SKSGAMNQSSSQKNTRKKSLKGNNKGIEKPPGKNSRQVPKSTKKGKYMAA
INGEKMQIGIGRGGSQTNTISSTGKTLAECIQHGGPMASMKMPSQKGLSG
DWALGKESSPGWSDMSMGTNTNSLLDDDQREFQEPSYILSNIASGMADVQ
RFMMASIEPLWEPMEHHGDPNIFYSPESNSLKLKTLKILAGTPQESKKKI
NSGSQGATKNHRSIKGVSKSNGKTAIGDPGRANMPGYNEDSRSTFFDKKY
SNMSTLGNNGPTHKKLYRHKSSSKALRDEKCKGKHMEREQVHKDESGTAS
FEKLRDSDYNLLKAETTFWVLPVFEEETRIFQKDI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU45878
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee