cDNA Clone NUAK1

• Synonyms: ARK5
• Plasmid: pEBG HA NUAK1 S445/481A
• Parent Plasmid: pEBG6P
• Expressed: GST-HA-NUAK1 S445/481A
• Genbank: NM_014840.2
• Tag: GST
• Tag: HA
• Species: Human

Sequence of Insert

ACTAGTATGTACCCATACGATGTGCCAGATTACGCCGAAGGGGCCGCCGCGCCTGTGGCGGGGGACCGCCCCGACTTGGGGCTGGGGGCGCCGGGCTCTCCCCGAGAGGCGGTGGCGGGGGCGACTGCAGCCCTGGAGCCCAGGAAGCCGCACGGGGTGAAGCGGCATCACCACAAGCACAACTTGAAGCACCGCTACGAGCTGCAGGAGACCCTGGGCAAAGGCACCTACGGCAAAGTCAAGCGGGCCACCGAGAGGTTTTCTGGCCGAGTGGTTGCTATAAAATCCATTCGTAAGGACAAAATTAAGGATGAACAAGACATGGTTCACATCAGACGAGAGATTGAGATCATGTCATCTCTCAACCATCCTCATATCATCAGTATTTATGAAGTGTTTGAGAACAAAGATAAGATTGTGATCATCATGGAATATGCCAGCAAAGGGGAGCTGTACGATTACATCAGTGAGCGGCGACGCCTCAGTGAGAGGGAGACCCGGCACTTCTTCCGGCAGATCGTCTCTGCTGTGCACTATTGTCACAAGAACGGTGTGGTCCACCGGGACTTGAAGCTGGAAAATATACTGCTCGATGACAACTGCAATATTAAGATTGCTGACTTTGGGCTTTCCAACCTGTACCAGAAGGATAAGTTCTTACAAACGTTTTGTGGGAGTCCACTCTATGCATCTCCTGAGATTGTCAATGGGAGACCTTACCGAGGGCCAGAGGTGGACAGCTGGGCCCTGGGTGTGTTGCTTTACACTCTTGTTTATGGAACAATGCCCTTCGATGGTTTCGATCACAAAAACCTCATTCGGCAAATCAGCAGCGGAGAGTACCGGGAGCCAACACAGCCCTCAGATGCTCGAGGACTCATACGGTGGATGCTGATGGTGAACCCCGATCGCCGGGCCACTATTGAGGACATTGCCAACCACTGGTGGGTGAACTGGGGCTATAAGAGCAGCGTGTGTGACTGTGATGCCCTCCATGACTCTGAGTCCCCACTCCTGGCTCGGATCATTGACTGGCACCACCGTTCCACAGGGCTGCAGGCTGACACCGAAGCCAAAATGAAGGGCCTGGCCAAACCCACGACCTCTGAGGTCATGCTAGAGCGGCAGCGGTCGCTGAAGAAATCCAAGAAAGAGAATGACTTTGCTCAGTCTGGTCAGGATGCAGTGCCTGAAAGCCCATCCAAGTTGAGTTCTAAGAGGCCCAAGGGGATCCTGAAGAAGCGAAGCAACAGCGAGCATCGCTCTCACAGCACTGGCTTCATTGAAGGTGTAGTTGGTCCTGCCTTACCCTCTACTTTCAAGATGGAGCAGGACTTGTGCAGGACTGGCGTGCTCCTCCCAAGCGCACCAGAGGCAGAGGTGCCGGGAAAACTCAGCCCCAAGCAGTCGGCCACGATGCCCAAGAAAGGCATCTTGAAAAAGACCCAGCAGAGAGAATCAGGTTACTACTCTGCCCCAGAGCGCAGTGAGTCTTCGGAGCTGTTGGACAGTAATGATGTGATGGGCAGCAGCATCCCCTCCCCCAGCCCCCCGGACCCAGCCAGGGTAACCTCCCACAGCCTCTCCTGCCGGAGGAAGGGCATCTTGAAACACAGCAGCAAATACTCAGCGGGCACCATGGACCCAGCCCTGGTCAGCCCTGAAATGCCCACACTGGAATCCCTGTCAGAGCCTGGTGTCCCTGCCGAGGGCCTCTCCCGGAGCTACAGCCGCCCTTCCAGTGTCATCAGCGATGACAGCGTGCTGTCCAGCGACTCTTTTGACTTGCTGGATTTGCAGGAGAATCGCCCTGCCCGCCAGCGCATCCGCAGCTGCGTCTCTGCAGAAAACTTCCTCCAGATCCAGGACTTTGAGGGGCTCCAGAACCGGCCCCGGCCCCAGTACCTGAAGCGGTACCGGAACCGGCTGGCAGACAGCAGCTTCTCCCTCCTCACAGACATGGATGATGTGACTCAGGTCTACAAGCAAGCGCTGGAGATCTGCAGCAAGCTCAACTAGAAGGGCGAATTCCCGGGTCGACTCGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSTSMYPYDVPDY
AEGAAAPVAGDRPDLGLGAPGSPREAVAGATAALEPRKPHGVKRHHHKHN
LKHRYELQETLGKGTYGKVKRATERFSGRVVAIKSIRKDKIKDEQDMVHI
RREIEIMSSLNHPHIISIYEVFENKDKIVIIMEYASKGELYDYISERRRL
SERETRHFFRQIVSAVHYCHKNGVVHRDLKLENILLDDNCNIKIADFGLS
NLYQKDKFLQTFCGSPLYASPEIVNGRPYRGPEVDSWALGVLLYTLVYGT
MPFDGFDHKNLIRQISSGEYREPTQPSDARGLIRWMLMVNPDRRATIEDI
ANHWWVNWGYKSSVCDCDALHDSESPLLARIIDWHHRSTGLQADTEAKMK
GLAKPTTSEVMLERQRSLKKSKKENDFAQSGQDAVPESPSKLSSKRPKGI
LKKRSNSEHRSHSTGFIEGVVGPALPSTFKMEQDLCRTGVLLPSAPEAEV
PGKLSPKQSATMPKKGILKKTQQRESGYYSAPERSESSELLDSNDVMGSS
IPSPSPPDPARVTSHSLSCRRKGILKHSSKYSAGTMDPALVSPEMPTLES
LSEPGVPAEGLSRSYSRPSSVISDDSVLSSDSFDLLDLQENRPARQRIRS
CVSAENFLQIQDFEGLQNRPRPQYLKRYRNRLADSSFSLLTDMDDVTQVY
KQALEICSKLN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44940
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: S445A and S481A