cDNA Clone IKKbeta

• Plasmid: pRetrox tight HA IKKb K44A S177E
• Parent Plasmid: pRetroX tight puro
• Expressed: HA-IKKbeta K44A S177E
• Genbank: NP_001547.1
• Tag: HA
• Species: Human

Sequence of Insert

gcggccgcgagctggtcaccttccctgacaacgcagacatgtggggcctg
ggaaatgaaagagcgccttgggacagggggatttggaaatgtcatccgat
ggcacaatcaggaaacaggtgagcagattgccatcGCgcagtgccggcag
gagctcagcccccggaaccgagagcggtggtgcctggagatccagatcat
gagaaggctgacccaccccaatgtggtggctgcccgagatgtccctgagg
ggatgcagaacttggcgcccaatgacctgcccctgctggccatggagtac
tgccaaggaggagatctccggaagtacctgaaccagtttgagaactgctg
tggtctgcgggaaggtgccatcctcaccttgctgagtgacattgcctctg
cgcttagataccttcatgaaaacagaatcatccatcgggatctaaagcca
gaaaacatcgtcctgcagcaaggagaacagaggttaatacacaaaattat
tgacctaggatatgccaaggagctggatcagggcGAActttgcacatcat
tcgtggggaccctgcagtacctggccccagagctactggagcagcagaag
tacacagtgaccgtcgactactggagcttcggcaccctggcctttgagtg
catcacgggcttccggcccttcctccccaactggcagcccgtgcagtggc
attcaaaagtgcggcagaagagtgaggtggacattgttgttagcgaagac
ttgaatggaacggtgaagttttcaagctctttaccctaccccaataatct
taacagtgtcctggctgagcgactggagaagtggctgcaactgatgctga
tgtggcacccccgacagaggggcacggatcccacgtatgggcccaatggc
tgcttcaaggccctggatgacatcttaaacttaaagctggttcatatctt
gaacatggtcacgggcaccatccacacctaccctgtgacagaggatgaga
gtctgcagagcttgaaggccagaatccaacaggacacgggcatcccagag
gaggaccaggagctgctgcaggaagcgggcctggcgttgatccccgataa
gcctgccactcagtgtatttcagacggcaagttaaatgagggccacacat
tggacatggatcttgtttttctctttgacaacagtaaaatcacctatgag
actcagatctccccacggccccaacctgaaagtgtcagctgtatccttca
agagcccaagaggaatctcgccttcttccagctgaggaaggtgtggggcc
aggtctggcacagcatccagaccctgaaggaagattgcaaccggctgcag
cagggacagcgagccgccatgatgaatctcctccgaaacaacagctgcct
ctccaaaatgaagaattccatggcttccatgtctcagcagctcaaggcca
agttggatttcttcaaaaccagcatccagattgacctggagaagtacagc
gagcaaaccgagtttgggatcacatcagataaactgctgctggcctggag
ggaaatggagcaggctgtggagctctgtgggcgggagaacgaagtgaaac
tcctggtagaacggatgatggctctgcagaccgacattgtggacttacag
aggagccccatgggccggaagcaggggggaacgctggacgacctagagga
gcaagcaagggagctgtacaggagactaagggaaaaacctcgagaccagc
gaactgagggtgacagtcaggaaatggtacggctgctgcttcaggcaatt
cagagcttcgagaagaaagtgcgagtgatctatacgcagctcagtaaaac
tgtggtttgcaagcagaaggcgctggaactgttgcccaaggtggaagagg
tggtgagcttaatgaatgaggatgagaagactgttgtccggctgcaggag
aagcggcagaaggagctctggaatctcctgaagattgcttgtagcaaggt
ccgtggtcctgtcagtggaagcccggatagcatgaatgcctctcgactta
gccagcctgggcagctgatgtctcagccctccacggcctccaacagctta
cctgagccagccaagaagagtgaagaactggtggctgaagcacataacct
ctgcaccctgctagaaaatgccatacaggacactgtgagggaacaagacc
agagtttcacggccctagactggagctggttacagacggaagaagaagag
cacagctgcctggagcaggcctcatgagcggccgc

Amino Acid Sequence

MYPYDVPDYAGSGEFGAAASWSPSLTTQTCGAWEMKERLGTGGFGNVIRW
HNQETGEQIAIAQCRQELSPRNRERWCLEIQIMRRLTHPNVVAARDVPEG
MQNLAPNDLPLLAMEYCQGGDLRKYLNQFENCCGLREGAILTLLSDIASA
LRYLHENRIIHRDLKPENIVLQQGEQRLIHKIIDLGYAKELDQGELCTSF
VGTLQYLAPELLEQQKYTVTVDYWSFGTLAFECITGFRPFLPNWQPVQWH
SKVRQKSEVDIVVSEDLNGTVKFSSSLPYPNNLNSVLAERLEKWLQLMLM
WHPRQRGTDPTYGPNGCFKALDDILNLKLVHILNMVTGTIHTYPVTEDES
LQSLKARIQQDTGIPEEDQELLQEAGLALIPDKPATQCISDGKLNEGHTL
DMDLVFLFDNSKITYETQISPRPQPESVSCILQEPKRNLAFFQLRKVWGQ
VWHSIQTLKEDCNRLQQGQRAAMMNLLRNNSCLSKMKNSMASMSQQLKAK
LDFFKTSIQIDLEKYSEQTEFGITSDKLLLAWREMEQAVELCGRENEVKL
LVERMMALQTDIVDLQRSPMGRKQGGTLDDLEEQARELYRRLREKPRDQR
TEGDSQEMVRLLLQAIQSFEKKVRVIYTQLSKTVVCKQKALELLPKVEEV
VSLMNEDEKTVVRLQEKRQKELWNLLKIACSKVRGPVSGSPDSMNASRLS
QPGQLMSQPSTASNSLPEPAKKSEELVAEAHNLCTLLENAIQDTVREQDQ
SFTALDWSWLQTEEEEHSCLEQAS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU46227
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: please insert HA-IKKbeta into pRETRO-X-tight. We need S177 and K44 mutated to Ala. Thank you.