cDNA Clone CHAF1A (chromatin assembly factor 1 subunit A (p150)

• Synonyms: CAF1, P150, CAF-1, CAF1B, CAF1P150, MGC71229
• Plasmid: pET24a-Chaf1a_TEV-6His
• Parent Plasmid: pET24a original
• Expressed: Chaf1a_TEV-6His
• Genbank: Gene ID 10036
• Tag: 6His
• Species: Human

Sequence of Insert

catatgctggaggagctggagtgcggggcgcccggcgccaggggagccgc
cacagccatggattgcaaagatagaccagcttttccagttaagaagttaa
tacaagcccgtctgccgtttaagcgcctgaatcttgtcccaaaggggaaa
gccgatgacatgtcagacgatcagggtacttctgtgcaaagtaaaagccc
cgatttagaggcctctttggacaccttggaaaacaactgtcatgtgggtt
ctgacatagactttagaccgaaacttgtcaacgggaagggtcccttagat
aactttttaagaaatagaatcgaaaccagtattggccagagcacagtcat
cattgatttgacagaggactcgaatgagcagccagacagtcttgtggacc
acaataaactaaattctgaagcctctccctccagggaggcaataaatggc
cagcgagaagacactggggatcagcaggggttgttgaaggccattcagaa
cgacaagttggcatttcctggagagaccctttcagacattccttgcaaaa
cagaggaggagggtgttggctgtggaggtgcagggaggagaggcgactcc
caggaatgttcgccacggagctgcccggagctgacgagtggcccgagaat
gtgccccagaaaggagcaggacagttggagtgaagctgggggcatcctgt
tcaaagggaaggtgcctatggtggtcttgcaggacatcttggctgtgaga
ccaccgcaaatcaagtcccttccagccacaccccaaggcaagaacatgac
ccctgagagtgaggtgctggaatctttccccgaagaagactctgtactca
gccattcgtccctgagctctccctcttccaccagctcgcccgaggggccg
cctgctcccccaaagcagcacagcagtaccagtcccttccccacctccac
gcccctccgcagaataactaagaaattcgtcaaaggctctacagagaaga
acaagctcagactgcaaagagatcaggagcgtctgggcaagcagctcaag
ttacgtgcagaaagggaagaaaaggagaagctgaaagaggaggccaagcg
ggccaaggaggaggccaagaagaagaaggaggaagagaaggagcttaagg
aaaaggagaggcgggagaagcgggagaaggatgagaaggagaaggcggag
aagcagcggctcaaggaggagcggcgcaaggagagacaggaagccctgga
ggctaaacttgaggaaaaaaggaaaaaggaagaagagaaacggttaagag
aagaagagaagcgcattaaagcagagaaggccgaaatcacgaggttcttc
cagaaaccaaagactccacaggcccccaagaccctggccggctcctgtgg
gaagtttgccccctttgaaattaaagagcacatggtcctggcccctcggc
gtcggaccgctttccatccagacctctgcagtcagctggaccagctcctc
cagcagcagagcggcgagttctccttcttgaaagacctcaaaggccggca
gcccctgaggtccggacccacgcacgtttccacccggaatgcagatattt
ttaacagtgatgtcgtcatcgtggagcgtgggaagggcgacggtgttccc
gagaggaggaagtttggcaggatgaagctcctgcagttctgtgagaacca
ccggcctgcctactggggtacctggaataagaagacggcactcatccgcg
cgcgagacccctgggcccaggacacgaagctcctggactatgaggtggac
agtgatgaggagtgggaagaagaggagcctggggagtccctgtcccacag
tgagggggatgatgatgacgacatgggagaggatgaagatgaggacgatg
gtttctttgtgccccatgggtacctgtctgaggacgaaggtgtgacagag
gagtgtgccgaccctgagaaccataaggtccgccagaaactgaaggccaa
ggagtgggacgagttcctggctaaggggaagcgctttcgcgtcctgcaac
ctgtgaagatcggctgcgtgtgggcggctgacagagactgcgcaggcgat
gacctgaaggtactgcagcagttcgcagcctgcttcctggagaccctgcc
ggcccaggaggagcagacgcccaaggcctccaagcgggagaggagagacg
agcagatcctggcccagctgctgccgctcctgcacggcaatgtgaacggg
agcaaggtcatcatccgggagttccaggagcactgccgccggggactgct
cagcaaccacaccggcagcccgcggagcccctccaccacctacctgcaca
cccccacccccagcgaggatgccgccatcccctctaagtcccggctcaag
cggctcatttccgagaactcagtgtatgagaagcggcctgacttcaggat
gtgctggtacgtgcacccgcaggtgctacagagcttccagcaggagcacc
tgcccgtgccgtgccagtggagctatgtgacatcggtgccctcggccccc
aaagaggacagtggcagcgtcccctccacggggcccagccagggcactcc
catctcgctgaagaggaagtcagcgggcagcatgtgcatcacccaattca
tgaagaagcgcaggcacgacggccagattggtgctgaagacatggacggc
ttccaggcagacacggaggaggaggaagaggaggagggcgactgtatgat
cgtggatgtcccggatgctgcggaggtccaagccccgtgtggagccgctt
ccggagctgggggtggtgtgggggtggacaccggcaaggccaccctgacc
gcgagcccactgggtgcatccGAAAACCTGTATTTTCAGGGCCTCGAG

Amino Acid Sequence

MLEELECGAPGARGAATAMDCKDRPAFPVKKLIQARLPFKRLNLVPKGKA
DDMSDDQGTSVQSKSPDLEASLDTLENNCHVGSDIDFRPKLVNGKGPLDN
FLRNRIETSIGQSTVIIDLTEDSNEQPDSLVDHNKLNSEASPSREAINGQ
REDTGDQQGLLKAIQNDKLAFPGETLSDIPCKTEEEGVGCGGAGRRGDSQ
ECSPRSCPELTSGPRMCPRKEQDSWSEAGGILFKGKVPMVVLQDILAVRP
PQIKSLPATPQGKNMTPESEVLESFPEEDSVLSHSSLSSPSSTSSPEGPP
APPKQHSSTSPFPTSTPLRRITKKFVKGSTEKNKLRLQRDQERLGKQLKL
RAEREEKEKLKEEAKRAKEEAKKKKEEEKELKEKERREKREKDEKEKAEK
QRLKEERRKERQEALEAKLEEKRKKEEEKRLREEEKRIKAEKAEITRFFQ
KPKTPQAPKTLAGSCGKFAPFEIKEHMVLAPRRRTAFHPDLCSQLDQLLQ
QQSGEFSFLKDLKGRQPLRSGPTHVSTRNADIFNSDVVIVERGKGDGVPE
RRKFGRMKLLQFCENHRPAYWGTWNKKTALIRARDPWAQDTKLLDYEVDS
DEEWEEEEPGESLSHSEGDDDDDMGEDEDEDDGFFVPHGYLSEDEGVTEE
CADPENHKVRQKLKAKEWDEFLAKGKRFRVLQPVKIGCVWAADRDCAGDD
LKVLQQFAACFLETLPAQEEQTPKASKRERRDEQILAQLLPLLHGNVNGS
KVIIREFQEHCRRGLLSNHTGSPRSPSTTYLHTPTPSEDAAIPSKSRLKR
LISENSVYEKRPDFRMCWYVHPQVLQSFQQEHLPVPCQWSYVTSVPSAPK
EDSGSVPSTGPSQGTPISLKRKSAGSMCITQFMKKRRHDGQIGAEDMDGF
QADTEEEEEEEGDCMIVDVPDAAEVQAPCGAASGAGGGVGVDTGKATLTA
SPLGASENLYFQGLEHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU24267
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Gibson Assembly method used. Oligos amplified Chaf1a_TEV fragment with overlaps to Nde/Xho cut pET24a