cDNA Clone BRD4 (mouse)

• Synonyms: HUNK1
• Plasmid: pGEX6P-1 BRD4 (mouse)
• Parent Plasmid: pGEX6P1
• Expressed: GST BRD4 (mouse)
• Genbank: XM_006524691.1
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCATGTCTACGGAGAGCGGCCCTGGGACAAGATTGAGAAATCTGCC
AGTAATGGGGGATGGACTAGAAACCTCCCAAATGTCTACAACGCAGGCCC
AGGCCCAACCCCAGCCAGCAAATGCAGCCAGCACCAATCCTCCACCCCCA
GAGACCTCCAACCCTAACAAGCCCAAGAGACAGACAAACCAACTGCAATA
TCTGCTCAGAGTGGTGCTCAAGACACTATGGAAACACCAGTTTGCGTGGC
CTTTCCAGCAGCCCGTGGATGCCGTCAAGCTGAACCTCCCTGATTACTAT
AAGATTATTAAAACACCCATGGATATGGGAACAATAAAGAAGCGCTTGGA
AAACAACTATTACTGGAATGCTCAGGAATGTATCCAGGACTTCAACACTA
TGTTTACAAATTGTTACATCTATAACAAGCCTGGAGATGACATCGTCTTA
ATGGCAGAAGCTCTGGAGAAGCTCTTCTTGCAAAAAATCAATGAACTGCC
TACAGAAGAAACTGAGATCATGATAGTCCAGGCAAAAGGAAGAGGACGAG
GGAGGAAAGAAACAGGGGCAGCAAAGCCTGGTGTATCCACGGTACCAAAC
ACAACTCAAGCATCAACTTCTCCGCAGACCCAGACGCCTCAGCAGAACCC
TCCTCCACCTGTGCAGGCCACAACTCACCCCTTTCCTGCTGTCACCCCAG
ACCTCATTGCCCAGCCTCCTGTCATGACAATGGTGCCCCCTCAGCCACTT
CAGACTCCTTCACCGGTACCCCCCCAGCCACCACCCCCACCTGCTCCAGT
TCCACAGCCTGTGCAGAGTCACCCGCCCATCATTGCGACCACCCCCCAGC
CTGTGAAGACAAAGAAAGGGGTGAAGAGGAAAGCAGATACCACCACCCCT
ACCACCATCGACCCCATTCATGAGCCACCCTCACTGGCCCCAGAGCCCAA
GACCGCCAAGCTGGGTCCTCGGCGGGAGAGCAGCAGACCTGTGAAGCCTC
CAAAGAAGGATGTACCGGACTCACAGCAGCACCCAGGGCCAGAGAAGAGC
AGCAAGATCTCTGAGCAGCTAAAGTGCTGCAGTGGCATCCTCAAGGAGAT
GTTTGCCAAGAAACATGCTGCCTATGCCTGGCCTTTCTACAAGCCTGTGG
ATGTGGAGGCACTGGGTCTGCACGACTACTGTGACATCATCAAACATCCC
ATGGACATGAGCACAATCAAGTCTAAACTAGAGTCCCGAGAGTACAGAGA
TGCCCAGGAATTTGGTGCTGATGTCCGATTGATGTTCTCCAACTGCTACA
AGTACAACCCCCCTGACCATGAAGTGGTAGCCATGGCTCGAAAACTCCAG
GATGTGTTTGAAATGCGCTTTGCCAAGATGCCTGATGAGCCTGAAGAGCC
AGTTGTTACAGTGTCCTCTCCTGCAGTGCCACCCCCTACAAAGGTGGTAG
CCCCACCCTCATCTAGTGACAGCAGCAGCGACAGTTCTTCCGACAGCGAC
AGTTCCACTGACGACTCTGAGGAAGAGCGAGCCCAGCGGCTGGCTGAACT
CCAGGAACAGCTCAAGGCCGTGCATGAGCAGCTTGCAGCCCTCTCACAGC
CCCAGCAGAACAAACCAAAGAAAAAGGAGAAGGACAAGAAGGAAAAGAAA
AAGGAAAAGCACAAAAAGAAAGAAGAAGTGGAGGAAAATAAAAAAAGCAA
AACCAAGGAACTTCCTCCCAAAAAGACAAAGAAAAATAACAGCAGCAACA
GCAATGTGAGCAAGAAGGAACCAGTACCCACGAAGACCAAGCCGCCTCCC
ACATATGAATCAGAAGAGGAGGATAAGTGTAAGCCCATGTCTTATGAGGA
GAAGCGGCAGCTAAGTCTAGATATCAACAAACTTCCTGGTGAGAAGCTAG
GCCGTGTAGTACACATAATTCAGTCAAGGGAACCATCACTTAAAAACTCC
AACCCCGATGAGATTGAGATTGACTTTGAGACCCTGAAGCCATCTACACT
ACGAGAGTTGGAGCGATATGTCACCTCCTGTTTGCGGAAGAAAAGGAAAC
CTCAAGCTGAAAAAGTTGACGTGATTGCTGGTTCTTCCAAGATGAAGGGA
TTCTCATCCTCTGAGTCGGAGAGCACCAGCGAATCCAGCTCCTCTGACAG
TGAAGACTCTGAAACAGAGATGGCTCCCAAGTCAAAAAAGAAGGGGCACA
CTGGGAGGGACCAGAAAAAGCATCATCACCATCACCATCCACAGATGCAG
CCAGCCCCAGCTCCTGTGCCCCAGCAGCCGCCCCCACCTCCACAGCAGCC
TCCTCCACCCCCACCTCCGCAGCAGCAGCAGCAGCAACCTCCACCCCCAC
CACCTCCGCCCTCCATGCCACAGCAGACTGCCCCAGCGATGAAGTCCTCG
CCCCCACCCTTCATCACTGCCCAGGTCCCCGTCCTGGAACCACAGCTGCC
AGGCAGTGTCTTTGACCCTATTGGCCACTTCACCCAGCCCATCTTGCACC
TGCCGCAGCCGGAGCTGCCTCCTCACCTGCCCCAGCCACCTGAGCACAGC
ACTCCACCCCATCTCAACCAGCATGCTGTGGTCTCTCCTCCAGCTTTGCA
CAATGCGCTGCCCCAACAGCCATCTCGGCCCAGTAACCGAGCTGCTGCTC
TGCCCCCAAAGCCTACCCGACCCCCAGCTGTGTCCCCTGCCCTGGCCCAG
CCCCCCCTGCTCCCACAACCACCAATGGCTCAGCCCCCCCAAGTGCTGCT
GGAGGATGAAGAGCCACCTGCCCCACCCCTCACCTCCATGCAGATGCAGT
TGTACCTACAGCAGCTGCAGAAGGTGCAGCCTCCCACACCACTACTCCCT
TCCGTGAAGGTGCAGTCCCAACCCCCACCGCCTTTGCCGCCTCCACCCCA
CCCTTCTGTGCAGCAGCAGCAGCTCCAGCCACAGCCACCGCCACCCCCAC
CTCCTCAGCCACAGCCACCACCCCAGCAACAACACCAGCCTCCACCACGA
CCAGTTCACTTGCCATCCATGCCCTTTTCTGCTCATATTCAGCAGCCCCC
ACCACCCCCAGGACAGCAACCTACTCACCCACCCCCAGGACAGCAGCCCC
CACCACCACAGCCTGCCAAGCCCCAGCAAGTCATCCAGCATCACCCTTCC
CCCCGGCACCACAAGTCAGACCCCTACTCAGCTGGTCATCTTCGTGAGGC
TCCCTCTCCGCTTATGATACATTCCCCTCAGATGCCACAGTTCCAGAGCC
TGACCCATCAGTCTCCTCCCCAGCAAAACGTCCAGCCTAAGAAGCAGGAG
CTGCGACCACCTTCAGTCGTCCAGCCCCAGCCCCTGGTGGTAGTAAAGGA
GGAGAAGATTCACTCACCAATCATTCGCAGCGAGCCTTTCAGCACCTCAC
TTCGACCAGAGCCCCCCAAGCACCCAGAGAACATCAAGGCCCCAGTCCAC
CTGCCCCAGCGGCCTGAGATGAAGCCTGTAGACATAGGGAGGCCTGTGAT
CCGGCCTCCAGAGCAGAGTGCACCCCCACCAGGGGCCCCTGACAAGGACA
AACAGAAGCAGGAGCCAAAAACACCAGTGGCACCCAAAAAGGACCTGAAA
ATTAAGAACATGGGCTCCTGGGCCAGCCTGGTACAGAAGCATCCGACCAC
CCCATCCTCCACAGCCAAGTCCTCAAGTGACAGCTTTGAGCATTTCCGCC
GTGCTGCTCGGGAGAAGGAGGAGAGGGAAAAGGCCCTGAAGGCTCAGGCT
GAGCATGCAGAGAAGGAGAAGGAGCGGCTGAGGCAGGAGCGCATGAGGAG
CCGAGAGGATGAAGATGCGCTGGAACAGGCACGTCGAGCCCATGAGGAGG
CACGACGGCGCCAGGAGCAGCAGCAGCAGCAGCAGCAGCAGAGACAGGAG
CAGCAGCAGCAGCAGCAGCAGGCAGCTGCTGTGGCCGCTGCCTCTGCCCC
CCAGGCCCAGAGCTCCCAGCCCCAGTCTATGCTGGACCAGCAGAGGGAGT
TGGCCCGGAAACGAGAGCAGGAGCGGAGGCGCAGGGAGGCAATGGCAGCT
ACAATTGACATGAATTTCCAGAGTGATCTTTTGTCAATATTTGAAGAAAA
TCTTTTTTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSTESGPGTRLRNLPVMGD
GLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRV
VLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYY
WNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEET
EIMIVQAKGRGRGRKETGAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPV
QATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPV
QSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKL
GPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKK
HAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEF
GADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTV
SSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQL
KAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKEL
PPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQL
SLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELE
RYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSE
TEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPP
PPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVF
DPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALP
QQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEE
PPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQ
QQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPG
QQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPL
MIHSPQMPQFQSLTHQSPPQQNVQPKKQELRPPSVVQPQPLVVVKEEKIH
SPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPE
QSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSST
AKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDE
DALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQS
SQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU46280
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee