cDNA Clone AREL1

• Plasmid: pcDNA5 FRT/TO 3xFLAG-AREL1
• Parent Plasmid: pcDNA5 FRT/TO 3FLAG1
• Expressed: 3xFLAG-AREL1
• Genbank: O15033
• Tag: 3FLAG
• Species: Human

Sequence of Insert

agatctATGTTTTACGTTATTGGTGGAATCACAGTGTCTGTGGTTGCATTCTTCTT
CACAATTAAGTTCCTCTTTGAGCTTGCCGCACGTGTAGTCAGCTTCCTCC
AGAATGAGGACCGCGAGCGCCGAGGGGACCGGACTATTTATGACTACGTG
CGGGGAAATTACCTGGATCCCCGGTCTTGCAAAGTCTCCTGGGATTGGAA
GGACCCCTATGAGGTGGGCCACAGCATGGCCTTCCGAGTGCATTTATTCT
ATAAGAACGGGCAGCCTTTCCCTGCACATCGGCCTGTGGGACTAAGAGTT
CACATCTCTCATGTCGAGCTAGCAGTGGAAATTCCAGTGACCCAGGAAGT
CCTTCAGGAGCCCAATTCCAACGTAGTAAAAGTGGCCTTCACTGTGCGCA
AGGCTGGGCGTTATGAAATCACAGTGAAGCTTGGTGGATTAAATGTGGCA
TATAGTCCCTACTACAAAATTTTTCAACCTGGAATGGTGGTTCCTTCTAA
GACCAAAATTGTGTGCCACTTTTCTACTCTTGTATTGACCTGTGGGCAGC
CGCACACCCTTCAAATAGTACCCCGAGATGAGTATGATAATCCCACCAAC
AATTCCATGTCCTTGAGAGATGAGCACAATTACACCTTGTCCATTCATGA
GCTCGGCCCTCAAGAAGAAGAGAGTACTGGTGTCTCATTTGAGAAATCAG
TAACATCCAACAGGCAGACTTTCCAGGTGTTCTTGCGACTCACCCTGCAT
TCTCGAGGCTGCTTCCATGCTTGCATTTCATACCAAAATCAGCCAATCAA
TAATGGTGAATTTGACATTATTGTCCTAAGTGAGGATGAGAAGAATATCG
TCGAACGCAATGTGTCCACTTCAGGCGTGAGCATTTACTTTGAGGCTTAT
CTTTATAATGCTACCAACTGTAGCAGCACTCCATGGCACCTGCCACCCAT
GCACATGACCTCTTCCCAGCGCCGGCCATCCACTGCTGTTGACGAGGAAG
ATGAAGACTCGCCCTCTGAGTGCCACACCCCTGAGAAGGTGAAGAAACCG
AAGAAGGTGTACTGCTATGTGTCACCAAAGCAATTCTCAGTGAAGGAGTT
CTACCTGAAGATCATCCCCTGGCGCCTTTACACCTTCCGAGTGTGTCCAG
GAACAAAATTTTCATACCTTGGTCCTGACCCTGTCCATAAGCTGCTCACA
CTGGTGGTGGATGATGGCATTCAACCTCCTGTGGAGCTCAGCTGTAAGGA
GAGGAACATTCTAGCAGCCACTTTTATCCGCTCCCTGCATAAGAACATAG
GAGGCTCTGAGACCTTTCAGGACAAGGTGAACTTTTTCCAGCGAGAGCTT
CGGCAGGTACATATGAAAAGACCACATTCCAAAGTCACCCTGAAGGTCAG
CAGACATGCCTTGTTGGAATCGTCTCTGAAAGCCACTCGGAATTTCTCCA
TCTCAGATTGGAGCAAGAACTTTGAGGTTGTTTTCCAGGATGAAGAAGCT
CTGGACTGGGGAGGGCCTCGCCGGGAATGGTTTGAGCTAATCTGCAAAGC
ACTATTTGATACCACCAATCAGCTCTTCACCCGGTTCAGTGACAACAACC
AAGCATTAGTGCATCCCAACCCTAATCGCCCCGCTCATCTGCGCCTGAAA
ATGTATGAGTTTGCGGGACGGCTCGTGGGCAAGTGTCTCTATGAGTCCTC
TCTAGGAGGAGCCTACAAGCAGTTGGTCCGAGCTCGCTTCACCCGCTCTT
TCCTGGCCCAAATCATAGGACTGCGTATGCATTACAAGTACTTTGAAACA
GATGACCCAGAATTCTACAAATCTAAAGTTTGTTTTATCCTCAACAATGA
CATGAGTGAGATGGAGCTGGTCTTTGCAGAAGAGAAATATAATAAATCAG
GTCAATTGGATAAGGTTGTAGAACTCATGACAGGTGGAGCTCAAACTCCA
GTCACCAATGCGAATAAAATCTTCTATTTAAATTTGCTGGCCCAATATCG
GCTGGCCAGTCAAGTGAAAGAGGAGGTGGAACATTTCCTAAAAGGCCTGA
ATGAATTGGTCCCTGAGAACCTTTTGGCTATTTTTGATGAGAATGAGCTT
GAGCTGCTGATGTGTGGGACTGGAGACATCAGTGTGTCTGACTTCAAAGC
CCATGCAGTAGTTGTTGGTGGCTCATGGCATTTCAGAGAAAAGGTCATGA
GGTGGTTTTGGACTGTGGTTTCCAGTCTGACCCAGGAGGAGTTGGCTCGG
CTACTTCAGTTCACAACAGGCTCCTCTCAGCTACCACCTGGAGGCTTTGC
CGCCCTCTGTCCCTCATTTCAGATTATTGCCGCTCCGACCCATAGCACGC
TGCCTACTGCACACACATGTTTTAACCAGCTGTGCCTCCCTACATATGAC
TCCTATGAAGAGGTGCACAGGATGCTGCAGCTGGCTATCAGCGAGGGTTG
CGAGGGCTTTGGCATGCTCTGAgcggccgc

Amino Acid Sequence

MDYKDDDDKGSDYKDDDDKDYKDDDDKGSMFYVIGGITVSVVAFFFTIKF
LFELAARVVSFLQNEDRERRGDRTIYDYVRGNYLDPRSCKVSWDWKDPYE
VGHSMAFRVHLFYKNGQPFPAHRPVGLRVHISHVELAVEIPVTQEVLQEP
NSNVVKVAFTVRKAGRYEITVKLGGLNVAYSPYYKIFQPGMVVPSKTKIV
CHFSTLVLTCGQPHTLQIVPRDEYDNPTNNSMSLRDEHNYTLSIHELGPQ
EEESTGVSFEKSVTSNRQTFQVFLRLTLHSRGCFHACISYQNQPINNGEF
DIIVLSEDEKNIVERNVSTSGVSIYFEAYLYNATNCSSTPWHLPPMHMTS
SQRRPSTAVDEEDEDSPSECHTPEKVKKPKKVYCYVSPKQFSVKEFYLKI
IPWRLYTFRVCPGTKFSYLGPDPVHKLLTLVVDDGIQPPVELSCKERNIL
AATFIRSLHKNIGGSETFQDKVNFFQRELRQVHMKRPHSKVTLKVSRHAL
LESSLKATRNFSISDWSKNFEVVFQDEEALDWGGPRREWFELICKALFDT
TNQLFTRFSDNNQALVHPNPNRPAHLRLKMYEFAGRLVGKCLYESSLGGA
YKQLVRARFTRSFLAQIIGLRMHYKYFETDDPEFYKSKVCFILNNDMSEM
ELVFAEEKYNKSGQLDKVVELMTGGAQTPVTNANKIFYLNLLAQYRLASQ
VKEEVEHFLKGLNELVPENLLAIFDENELELLMCGTGDISVSDFKAHAVV
VGGSWHFREKVMRWFWTVVSSLTQEELARLLQFTTGSSQLPPGGFAALCP
SFQIIAAPTHSTLPTAHTCFNQLCLPTYDSYEEVHRMLQLAISEGCEGFG
ML*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24272
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: silent change at Ala812 compared to NCBI ref (GCT instead of GCC); ; parental 45919