cDNA Clone DCUN1D5

• Synonyms: DCN1, defective in cullin neddylation 1, domain containing 5
• Plasmid: pET15 6HIS SUMO DCUN1D5
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6HIS SUMO DCUN1D5
• Genbank: NM_032299.3.
• Tag: 6His
• Tag: SUMO
• Species: Human

Sequence of Insert

ggatccATGCCGGTGAAGAAGAAGAGAAAATCCCCTGGGGTGGCAGCAGCAGTAGCGGAAGACGGAGGCCTCAAAAAGTGTAAAATCTCCAGCTATTGCAGATCCCAACCCCCTGCTAGACTAATAAGTGGAGAGGAACATTTTTCAAGCAAGAAGTGCCTGGCTTGGTTTTATGAATATGCAGGTCCTGATGAAGTTGTAGGGCCAGAAGGAATGGAAAAATTTTGTGAAGACATTGGTGTTGAACCTGAAAATATTATTATGTTAGTTTTAGCGTGGAAATTGGAGGCTGAAAGCATGGGATTTTTTACCAAGGAAGAATGGTTAAAGGGAATGACTTCATTACAGTGTGACTGCACAGAAAAGTTACAAAACAAATTTGACTTTTTGCGCTCACAGTTGAATGATATTTCGTCATTTAAGAATATCTACAGATATGCCTTTGATTTTGCAAGGGATAAAGATCAGAGAAGCCTTGATATTGATACTGCTAAATCTATGTTAGCTCTTCTGCTTGGGAGGACATGGCCACTGTTTTCAGTATTTTACCAGTACCTGGAGCAATCAAAGTATCGTGTTATGAACAAAGATCAATGGTACAATGTATTAGAATTCAGCAGAACAGTCCATGCTGATCTTAGTAACTATGATGAAGATGGTGCTTGGCCTGTTCTTCTTGATGAATTTGTTGAGTGGCAAAAAGTCCGTCAGACATCATAGGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGSMPVKKKRKSPGVAAAVAEDGGLKKCKISSYCRSQPPARLISGEEHFSSKKCLAWFYEYAGPDEVVGPEGMEKFCEDIGVEPENIIMLVLAWKLEAESMGFFTKEEWLKGMTSLQCDCTEKLQNKFDFLRSQLNDISSFKNIYRYAFDFARDKDQRSLDIDTAKSMLALLLGRTWPLFSVFYQYLEQSKYRVMNKDQWYNVLEFSRTVHADLSNYDEDGAWPVLLDEFVEWQKVRQTS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU25315
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Needs mutated to deleted linker after senp cleavage; Parental Plasmid DU32993