cDNA Clone MYSM1

• Synonyms: 2A-DUB; 2ADUB; RP4-592A1.1
• Plasmid: pcDNA5D FRT/TO GFP MYSM1
• Parent Plasmid: pcDNA5D FRT/TO GFP
• Expressed: GFP-MYSM1
• Genbank: NM_001085487.2 GI:328927063 with silent changes a594g, c735t and a1275g.
• Tag: GFP
• Species: Human

Sequence of Insert

AGATCTATGGCGGCTGAAGAGGCGGATGTGGATATCGAAGGGGACGTGGTAGCGGCGGCGGGGGCACAGCCAGGAAGTGGTGAAAATACAGCATCAGTTTTACAAAAAGATCACTATCTTGATTCATCTTGGAGAACAGAGAATGGCCTTATTCCTTGGACCTTGGATAACACCATCAGTGAAGAGAACAGAGCTGTTATTGAGAAAATGTTGTTGGAAGAAGAATATTATTTATCTAAAAAATCACAACCGGAAAAAGTCTGGCTTGATCAAAAGGAAGATGATAAAAAATACATGAAGAGTCTGCAGAAAACAGCAAAAATCATGGTACACTCTCCTACAAAACCAGCCAGTTACTCAGTAAAGTGGACGATAGAAGAAAAAGAGCTGTTTGAACAAGGGCTGGCTAAATTTGGCCGAAGATGGACCAAAATTTCAAAGCTAATTGGAAGCCGCACTGTTTTACAAGTGAAGAGTTATGCAAGACAGTATTTTAAAAATAAGGTCAAATGCGGTCTGGATAAAGAAACACCAAATCAGAAGACCGGCCATAATCTTCAAGTTAAAAATGAAGATAAAGGGACAAAGGCATGGACACCGTCATGTTTAAGGGGACGTGCTGATCCCAACTTGAATGCTGTAAAAATTGAAAAGTTATCTGATGATGAAGAAGTAGACATCACAGATGAGGTGGACGAGTTGTCTTCTCAAACACCCCAGAAGAATTCTAGCAGTGATCTTTTGTTAGACTTTCCTAATAGTAAAATGCATGAAACCAATCAAGGAGAATTCATTACTTCTGACAGCCAGGAAGCTCTCTTTTCTAAGTCTTCCAGGGGCTGTCTTCAAAATGAAAAGCAAGATGAAACACTTTCAAGCTCAGAAATTACACTGTGGACTGAGAAACAGAGCAATGGTGACAAAAAATCAATTGAATTAAATGACCAGAAATTTAATGAATTGATTAAAAACTGCAACAAGCATGATGGAAGGGGAATAATAGTTGATGCCAGGCAGTTGCCTTCTCCAGAGCCTTGTGAAATTCAGAAAAATTTGAATGATAATGAAATGCTTTTTCATTCTTGCCAAATGGTAGAGGAAAGCCATGAGGAAGAAGAGCTTAAGCCACCAGAACAGGAAATAGAAATAGATAGAAATATCATTCAAGAAGAAGAAAAACAAGCAATTCCTGAGTTTTTTGAGGGGCGCCAAGCTAAAACACCAGAACGCTATTTGAAAATTAGAAATTATATTTTGGATCAATGGGAGATATGCAAACCGAAATACTTAAATAAGACCTCAGTACGTCCTGGCCTGAAGAACTGTGGAGATGTTAATTGTATTGGACGGATTCATACATACCTCGAATTGATAGGAGCAATCAATTTTGGATGTGAACAGGCTGTGTATAATAGGCCACAAACAGTTGACAAAGTACGAATCAGAGACAGAAAAGATGCAGTAGAAGCATACCAACTTGCCCAGCGTCTGCAGTCTATGCGTACAAGGAGACGTAGGGTCCGAGACCCATGGGGAAACTGGTGTGATGCAAAGGACTTAGAAGGACAAACGTTTGAGCATCTCTCTGCTGAGGAGTTGGCAAAAAGAAGAGAAGAGGAAAAAGGCAGACCTGTTAAATCTTTAAAAGTGCCAAGACCAACAAAAAGCTCGTTTGATCCCTTCCAACTGATACCTTGTAATTTTTTTAGTGAAGAAAAGCAGGAGCCATTTCAGGTGAAAGTGGCTTCAGAAGCACTTTTAATAATGGATTTGCATGCTCATGTTTCTATGGCAGAAGTGATTGGTCTGTTAGGAGGAAGATACTCAGAAGTTGATAAAGTAGTTGAAGTCTGTGCAGCAGAACCATGTAACAGTCTGAGTACAGGACTACAGTGTGAGATGGATCCTGTATCACAAACACAGGCCTCAGAAACCTTGGCTGTTAGAGGCTTCAGTGTTATTGGATGGTATCATTCTCATCCTGCTTTTGATCCTAATCCTTCCTTACGAGATATTGACACACAAGCTAAATACCAGAGTTACTTCTCCAGAGGAGGTGCAAAGTTCATTGGGATGATTGTTAGTCCCTATAATCGAAATAATCCCTTACCATATTCTCAGATTACCTGCCTGGTTATAAGTGAGGAAATTAGCCCAGATGGCTCTTATCGCTTACCTTACAAATTTGAAGTACAGCAGATGTTAGAAGAACCTCAGTGGGGATTAGTATTTGAAAAGACAAGATGGATAATAGAAAAATACAGGCTCTCCCATAGCAGCGTCCCCATGGATAAAATCTTTCGCCGGGATTCTGACCTGACTTGTTTGCAGAAACTTTTGGAGTGTATGAGGAAGACTCTGAGCAAAGTGACCAATTGCTTTATGGCTGAAGAATTCTTGACTGAAATAGAAAATTTGTTCCTTTCCAATTATAAAAGCAACCAAGAGAATGGAGTAACCGAAGAGAACTGTACAAAGGAATTGTTAATGTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMAAEEA
DVDIEGDVVAAAGAQPGSGENTASVLQKDHYLDSSWRTENGLIPWTLDNT
ISEENRAVIEKMLLEEEYYLSKKSQPEKVWLDQKEDDKKYMKSLQKTAKI
MVHSPTKPASYSVKWTIEEKELFEQGLAKFGRRWTKISKLIGSRTVLQVK
SYARQYFKNKVKCGLDKETPNQKTGHNLQVKNEDKGTKAWTPSCLRGRAD
PNLNAVKIEKLSDDEEVDITDEVDELSSQTPQKNSSSDLLLDFPNSKMHE
TNQGEFITSDSQEALFSKSSRGCLQNEKQDETLSSSEITLWTEKQSNGDK
KSIELNDQKFNELIKNCNKHDGRGIIVDARQLPSPEPCEIQKNLNDNEML
FHSCQMVEESHEEEELKPPEQEIEIDRNIIQEEEKQAIPEFFEGRQAKTP
ERYLKIRNYILDQWEICKPKYLNKTSVRPGLKNCGDVNCIGRIHTYLELI
GAINFGCEQAVYNRPQTVDKVRIRDRKDAVEAYQLAQRLQSMRTRRRRVR
DPWGNWCDAKDLEGQTFEHLSAEELAKRREEEKGRPVKSLKVPRPTKSSF
DPFQLIPCNFFSEEKQEPFQVKVASEALLIMDLHAHVSMAEVIGLLGGRY
SEVDKVVEVCAAEPCNSLSTGLQCEMDPVSQTQASETLAVRGFSVIGWYH
SHPAFDPNPSLRDIDTQAKYQSYFSRGGAKFIGMIVSPYNRNNPLPYSQI
TCLVISEEISPDGSYRLPYKFEVQQMLEEPQWGLVFEKTRWIIEKYRLSH
SSVPMDKIFRRDSDLTCLQKLLECMRKTLSKVTNCFMAEEFLTEIENLFL
SNYKSNQENGVTEENCTKELLM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU49133
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee