cDNA Clone BRD4 (mouse)

• Synonyms: HUNK1
• Plasmid: pRETROX tight HA BRD4 (mouse)
• Parent Plasmid: pRetroX tight puro
• Expressed: HA BRD4 (mouse)
• Genbank: XM_006524691.1
• Tag: HA
• Species: Mouse

Sequence of Insert

GGATCCATGTCTACGGAGAGCGGCCCTGGGACAAGATTGAGAAATCTGCC AGTAATGGGGGATGGACTAGAAACCTCCCAAATGTCTACAACGCAGGCCC AGGCCCAACCCCAGCCAGCAAATGCAGCCAGCACCAATCCTCCACCCCCA GAGACCTCCAACCCTAACAAGCCCAAGAGACAGACAAACCAACTGCAATA TCTGCTCAGAGTGGTGCTCAAGACACTATGGAAACACCAGTTTGCGTGGC CTTTCCAGCAGCCCGTGGATGCCGTCAAGCTGAACCTCCCTGATTACTAT AAGATTATTAAAACACCCATGGATATGGGAACAATAAAGAAGCGCTTGGA AAACAACTATTACTGGAATGCTCAGGAATGTATCCAGGACTTCAACACTA TGTTTACAAATTGTTACATCTATAACAAGCCTGGAGATGACATCGTCTTA ATGGCAGAAGCTCTGGAGAAGCTCTTCTTGCAAAAAATCAATGAACTGCC TACAGAAGAAACTGAGATCATGATAGTCCAGGCAAAAGGAAGAGGACGAG GGAGGAAAGAAACAGGGGCAGCAAAGCCTGGTGTATCCACGGTACCAAAC ACAACTCAAGCATCAACTTCTCCGCAGACCCAGACGCCTCAGCAGAACCC TCCTCCACCTGTGCAGGCCACAACTCACCCCTTTCCTGCTGTCACCCCAG ACCTCATTGCCCAGCCTCCTGTCATGACAATGGTGCCCCCTCAGCCACTT CAGACTCCTTCACCGGTACCCCCCCAGCCACCACCCCCACCTGCTCCAGT TCCACAGCCTGTGCAGAGTCACCCGCCCATCATTGCGACCACCCCCCAGC CTGTGAAGACAAAGAAAGGGGTGAAGAGGAAAGCAGATACCACCACCCCT ACCACCATCGACCCCATTCATGAGCCACCCTCACTGGCCCCAGAGCCCAA GACCGCCAAGCTGGGTCCTCGGCGGGAGAGCAGCAGACCTGTGAAGCCTC CAAAGAAGGATGTACCGGACTCACAGCAGCACCCAGGGCCAGAGAAGAGC AGCAAGATCTCTGAGCAGCTAAAGTGCTGCAGTGGCATCCTCAAGGAGAT GTTTGCCAAGAAACATGCTGCCTATGCCTGGCCTTTCTACAAGCCTGTGG ATGTGGAGGCACTGGGTCTGCACGACTACTGTGACATCATCAAACATCCC ATGGACATGAGCACAATCAAGTCTAAACTAGAGTCCCGAGAGTACAGAGA TGCCCAGGAATTTGGTGCTGATGTCCGATTGATGTTCTCCAACTGCTACA AGTACAACCCCCCTGACCATGAAGTGGTAGCCATGGCTCGAAAACTCCAG GATGTGTTTGAAATGCGCTTTGCCAAGATGCCTGATGAGCCTGAAGAGCC AGTTGTTACAGTGTCCTCTCCTGCAGTGCCACCCCCTACAAAGGTGGTAG CCCCACCCTCATCTAGTGACAGCAGCAGCGACAGTTCTTCCGACAGCGAC AGTTCCACTGACGACTCTGAGGAAGAGCGAGCCCAGCGGCTGGCTGAACT CCAGGAACAGCTCAAGGCCGTGCATGAGCAGCTTGCAGCCCTCTCACAGC CCCAGCAGAACAAACCAAAGAAAAAGGAGAAGGACAAGAAGGAAAAGAAA AAGGAAAAGCACAAAAAGAAAGAAGAAGTGGAGGAAAATAAAAAAAGCAA AACCAAGGAACTTCCTCCCAAAAAGACAAAGAAAAATAACAGCAGCAACA GCAATGTGAGCAAGAAGGAACCAGTACCCACGAAGACCAAGCCGCCTCCC ACATATGAATCAGAAGAGGAGGATAAGTGTAAGCCCATGTCTTATGAGGA GAAGCGGCAGCTAAGTCTAGATATCAACAAACTTCCTGGTGAGAAGCTAG GCCGTGTAGTACACATAATTCAGTCAAGGGAACCATCACTTAAAAACTCC AACCCCGATGAGATTGAGATTGACTTTGAGACCCTGAAGCCATCTACACT ACGAGAGTTGGAGCGATATGTCACCTCCTGTTTGCGGAAGAAAAGGAAAC CTCAAGCTGAAAAAGTTGACGTGATTGCTGGTTCTTCCAAGATGAAGGGA TTCTCATCCTCTGAGTCGGAGAGCACCAGCGAATCCAGCTCCTCTGACAG TGAAGACTCTGAAACAGAGATGGCTCCCAAGTCAAAAAAGAAGGGGCACA CTGGGAGGGACCAGAAAAAGCATCATCACCATCACCATCCACAGATGCAG CCAGCCCCAGCTCCTGTGCCCCAGCAGCCGCCCCCACCTCCACAGCAGCC TCCTCCACCCCCACCTCCGCAGCAGCAGCAGCAGCAACCTCCACCCCCAC CACCTCCGCCCTCCATGCCACAGCAGACTGCCCCAGCGATGAAGTCCTCG CCCCCACCCTTCATCACTGCCCAGGTCCCCGTCCTGGAACCACAGCTGCC AGGCAGTGTCTTTGACCCTATTGGCCACTTCACCCAGCCCATCTTGCACC TGCCGCAGCCGGAGCTGCCTCCTCACCTGCCCCAGCCACCTGAGCACAGC ACTCCACCCCATCTCAACCAGCATGCTGTGGTCTCTCCTCCAGCTTTGCA CAATGCGCTGCCCCAACAGCCATCTCGGCCCAGTAACCGAGCTGCTGCTC TGCCCCCAAAGCCTACCCGACCCCCAGCTGTGTCCCCTGCCCTGGCCCAG CCCCCCCTGCTCCCACAACCACCAATGGCTCAGCCCCCCCAAGTGCTGCT GGAGGATGAAGAGCCACCTGCCCCACCCCTCACCTCCATGCAGATGCAGT TGTACCTACAGCAGCTGCAGAAGGTGCAGCCTCCCACACCACTACTCCCT TCCGTGAAGGTGCAGTCCCAACCCCCACCGCCTTTGCCGCCTCCACCCCA CCCTTCTGTGCAGCAGCAGCAGCTCCAGCCACAGCCACCGCCACCCCCAC CTCCTCAGCCACAGCCACCACCCCAGCAACAACACCAGCCTCCACCACGA CCAGTTCACTTGCCATCCATGCCCTTTTCTGCTCATATTCAGCAGCCCCC ACCACCCCCAGGACAGCAACCTACTCACCCACCCCCAGGACAGCAGCCCC CACCACCACAGCCTGCCAAGCCCCAGCAAGTCATCCAGCATCACCCTTCC CCCCGGCACCACAAGTCAGACCCCTACTCAGCTGGTCATCTTCGTGAGGC TCCCTCTCCGCTTATGATACATTCCCCTCAGATGCCACAGTTCCAGAGCC TGACCCATCAGTCTCCTCCCCAGCAAAACGTCCAGCCTAAGAAGCAGGAG CTGCGACCACCTTCAGTCGTCCAGCCCCAGCCCCTGGTGGTAGTAAAGGA GGAGAAGATTCACTCACCAATCATTCGCAGCGAGCCTTTCAGCACCTCAC TTCGACCAGAGCCCCCCAAGCACCCAGAGAACATCAAGGCCCCAGTCCAC CTGCCCCAGCGGCCTGAGATGAAGCCTGTAGACATAGGGAGGCCTGTGAT CCGGCCTCCAGAGCAGAGTGCACCCCCACCAGGGGCCCCTGACAAGGACA AACAGAAGCAGGAGCCAAAAACACCAGTGGCACCCAAAAAGGACCTGAAA ATTAAGAACATGGGCTCCTGGGCCAGCCTGGTACAGAAGCATCCGACCAC CCCATCCTCCACAGCCAAGTCCTCAAGTGACAGCTTTGAGCATTTCCGCC GTGCTGCTCGGGAGAAGGAGGAGAGGGAAAAGGCCCTGAAGGCTCAGGCT GAGCATGCAGAGAAGGAGAAGGAGCGGCTGAGGCAGGAGCGCATGAGGAG CCGAGAGGATGAAGATGCGCTGGAACAGGCACGTCGAGCCCATGAGGAGG CACGACGGCGCCAGGAGCAGCAGCAGCAGCAGCAGCAGCAGAGACAGGAG CAGCAGCAGCAGCAGCAGCAGGCAGCTGCTGTGGCCGCTGCCTCTGCCCC CCAGGCCCAGAGCTCCCAGCCCCAGTCTATGCTGGACCAGCAGAGGGAGT TGGCCCGGAAACGAGAGCAGGAGCGGAGGCGCAGGGAGGCAATGGCAGCT ACAATTGACATGAATTTCCAGAGTGATCTTTTGTCAATATTTGAAGAAAA TCTTTTTTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPA
NAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVD
AVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYI
YNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGA
AKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPP
VMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKG
VKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPD
SQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGL
HDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDH
EVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSD
SSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPK
KKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKE
PVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHII
QSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVD
VIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKK
HHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMP
QQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELP
PHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTR
PPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQ
KVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPP
PQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAK
PQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPP
QQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVV
QPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEM
KPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSW
ASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEK
ERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQ
AAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQ
SDLLSIFEENLF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU46334
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: in pRETROX tight