cDNA Clone DCUN1D1

• Synonyms: (Defective in cullin neddylation 1, domain containing 1)
• Plasmid: pET156P-1-DCUN1D1 K24R K36R
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-DCUN1D1 K24R K36R
• Genbank: (Defective in cullin neddylation 1, domain containing 1)
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGAACAAGTTGAAATCATCGCAGAAGGATAAAGTTCGTCAGTT
TATGATCTTCACACAATCTAGTGAAAgAACAGCAGTAAGTTGTCTTTCTC
AAAATGACTGGAgGTTAGATGTTGCAACAGATAATTTTTTCCAAAATCCT
GAACTTTATATACGAGAGAGTGTAAAAGGATCATTGGACAGGAAGAAGTT
AGAACAGCTGTACAATAGATACAAAGACCCTCAAGATGAGAATAAAATTG
GAATAGATGGCATACAGCAGTTCTGTGATGACCTGGCACTCGATCCAGCC
AGCATTAGTGTGTTGATTATTGCATGGAAGTTCAGAGCAGCAACACAGTG
CGAGTTCTCCAAACAGGAGTTCATGGATGGCATGACAGAATTAGGATGTG
ACAGCATAGAAAAACTAAAGGCCCAGATACCCAAGATGGAACAAGAATTG
AAAGAACCAGGACGATTTAAGGATTTTTACCAGTTTACTTTTAATTTTGC
AAAGAATCCAGGACAAAAAGGATTAGATCTAGAAATGGCCATTGCCTACT
GGAACTTAGTGCTTAATGGAAGATTTAAATTCTTAGACTTATGGAATAAA
TTTTTGTTGGAACATCATAAACGATCAATACCAAAAGACACTTGGAATCT
TCTTTTAGACTTCAGTACGATGATTGCAGATGACATGTCTAATTATGATG
AAGAAGGAGCATGGCCTGTTCTTATTGATGACTTTGTGGAATTTGCACGC
CCTCAAATTGCTGGGACAAAAAGTACAACAGTGTAGGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSISLEGLFQGPGSMNKLKSSQKDKVRQFMIFTQSSERTAV
SCLSQNDWRLDVATDNFFQNPELYIRESVKGSLDRKKLEQLYNRYKDPQD
ENKIGIDGIQQFCDDLALDPASISVLIIAWKFRAATQCEFSKQEFMDGMT
ELGCDSIEKLKAQIPKMEQELKEPGRFKDFYQFTFNFAKNPGQKGLDLEM
AIAYWNLVLNGRFKFLDLWNKFLLEHHKRSIPKDTWNLLLDFSTMIADDM
SNYDEEGAWPVLIDDFVEFARPQIAGTKSTTV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU24432
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: carries g318a mutation (silent Ala106)