cDNA Clone MYSM1

• Synonyms: 2A-DUB, 2ADUB
• Plasmid: pMK-RQ MYSM1 Cter GFP donor
• Parent Plasmid: pMK-RQ
• Expressed: MYSM1-GFP (following recombination)
• Genbank: ENSG00000162601
• Tag: GFP
• Species: Human

Sequence of Insert

ACTCCTTTCTGTTATATCCAGTTTTTTGTGTGTTGTTGAAAAAGTATGTGCACATTTCCAAGGAAATAGACTCCAGCTAGCATCCTCATTTTGAAATGCAGAATCCTATGTTTTTATGTACCTAGATTTTTGTGTGTGTGTATACTCAGGATTATTTTGCTTTGTAAGTGTAACTACAAATGGAGAAAAGTCGCAAATACGTCCGAAGTAGAAAGCATCACATAAACAATCTCAGGTTTGTCTCCTTATGTAAAATCATAAAAGTACTAAGCTGAGTGTTCCTCAGCAGCAATTTCCTACATTCCAGCTCTGTGACTATTAAACACAAGTTAATCTTCAACCATTTTTAAGGAGAGTGTTTCTCATTTGAGCATTAGGAAATGTGAGGTCTGGAAAGGGACGAAATAGTGATAGTAATGCAAACCCTCATACTTTTTCTGTATTTAAATATAAAACTTTTCTTTTCAACTAGCTTTTGGAGTGTATGAGGAAGACTCTGtctAAgGTcACtAAcTGtTTcATGGCTGAAGAATTCTTGACTGAAATAGAAAATTTGTTCCTTTCCAATTATAAAAGCAACCAAGAGAATGGAGTAACCGAAGAGAACTGTACAAAGGAATTGTTAATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTGATTATTTTAAAGTTAAGACATTTTAATCTTGACACAGTAGATCTTACTTTCAAAGTTATAAACTTGAAGTGATTGTAGTTAACATTGGCACAGCTTTGGTATTTTTTCTCTATTTCACAAAATCCAAACTTTGCCACATAAATCATGTGAAAAGGAAGAGATACAAATTTGTTTTCATATAGTGATTATCAGAGTGTTCTGCAAACCAGGGTCCACCACGGTGTTCTAGTCCTTTACTGAGCAATGCTGTAGGCTGTGAAACTAAGCAACCTGGTCGGCTTTACTGTTGTGTTCAGCTTGGGATTTGGACTATGTCTCTAAGTCATTTCTTCCCTGAACTAGTCATTTATGTTCCATTGTGGTATCTCCTATTTCATTTAAAAGCACTTCCATTCTTTCCATATATTCTGCTGAGATTTGTTTATGGCTATAAATGAGATATGGATGGGTGGTCACTGAGATAATTAATATGGTTTAGAACTACTGATTAATAAATCT

Amino Acid Sequence

MAAEEADVDIEGDVVAAAGAQPGSGENTASVLQKDHYLDSSWRTENGLIP
WTLDNTISEENRAVIEKMLLEEEYYLSKKSQPEKVWLDQKEDDKKYMKSL
QKTAKIMVHSPTKPASYSVKWTIEEKELFEQGLAKFGRRWTKISKLIGSR
TVLQVKSYARQYFKNKVKCGLDKETPNQKTGHNLQVKNEDKGTKAWTPSC
LRGRADPNLNAVKIEKLSDDEEVDITDEVDELSSQTPQKNSSSDLLLDFP
NSKMHETNQGEFITSDSQEALFSKSSRGCLQNEKQDETLSSSEITLWTEK
QSNGDKKSIELNDQKFNELIKNCNKHDGRGIIVDARQLPSPEPCEIQKNL
NDNEMLFHSCQMVEESHEEEELKPPEQEIEIDRNIIQEEEKQAIPEFFEG
RQAKTPERYLKIRNYILDQWEICKPKYLNKTSVRPGLKNCGDVNCIGRIH
TYLELIGAINFGCEQAVYNRPQTVDKVRIRDRKDAVEAYQLAQRLQSMRT
RRRRVRDPWGNWCDAKDLEGQTFEHLSAEELAKRREEEKGRPVKSLKVPR
PTKSSFDPFQLIPCNFFSEEKQEPFQVKVASEALLIMDLHAHVSMAEVIG
LLGGRYSEVDKVVEVCAAEPCNSLSTGLQCEMDPVSQTQASETLAVRGFS
VIGWYHSHPAFDPNPSLRDIDTQAKYQSYFSRGGAKFIGMIVSPYNRNNP
LPYSQITCLVISEEISPDGSYRLPYKFEVQQMLEEPQWGLVFEKTRWIIE
KYRLSHSSVPMDKIFRRDSDLTCLQKLLECMRKTLSKVTNCFMAEEFLTE
IENLFLSNYKSNQENGVTEENCTKELLMVSKGEELFTGVVPILVELDGDV
NGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFS
RYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNR
IELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIE
DGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU48228
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Designed to insert an in-frame GFP to the c-terminus of MYSM1 via CRISPR. Complete sequence of donor including homology arms shown as sequence of insert, contains several silent mutations to prevent Cas9 targeting the donor sequence. Sequence of cassette and amino acid sequence depict the expected fusion product following successful recombination. To be used in conjunction with 48211.